Determination of phenotypic and genotypic patterns of antibiotic resistance in uropathogenic Escherichia coli strains isolated from clinical samples in Shahrekord.

Subject Areas : microbiology

Amin Roozbehi ^{1 *}

1 -

Received: 2025-08-21 Accepted : 2025-09-24 Published : 2025-09-24

Keywords: Antibiotic resistance, Phenotypic pattern, Uropathogenic E. coli, ,

Abstract :

Escherichia coli is one of the most common microbial causes of urinary tract infections. Antibiotic resistance is one of the biggest causes of failure in antibiotic treatment due to the widespread use of antibiotics. The aim of this study was to determine the phenotypic and genotypic pattern of antibiotic resistance in uropathogenic Escherichia coli isolated from clinical samples in Shahrekord city. This descriptive-analytical and cross-sectional study was conducted on 130 patients with urinary tract infections referred to medical diagnostic laboratories in Shahrekord city. All isolates were confirmed using biochemical methods and molecular techniques based on 16srRNA gene detection, and the antibiotic resistance of the isolates was investigated using the disk diffusion method and molecular methods based on (qnr, tet A, tet B, aac (3)IIa and sul1) genes.

Of the 130 samples studied in the study of antimicrobial resistance pattern, the highest resistance was to ampicillin (96.67%) and the lowest resistance was to nitrofurantoin (53.1%). The aac (3)IIa gene (encoding resistance to gentamicin) with a frequency of 95.86% and the qnr gene (encoding resistance to ciprofloxacin) with a frequency of 58.14% were the highest and lowest antibiotic resistance genes detected in E. coli isolates. These results indicate an increase in E. coli resistance to gentamicin and tetracycline antibiotics compared to previous studies. Further research in this field will increase our knowledge and more effectively confront antibiotic resistance of pathogenic microorganisms.

References:

Servais P, Passerat J. Antimicrobial resistance of fecal bacteria in waters of the Seine river watershed (France). Science of The Total Environment. 2009; 408 (2): 356-372.
Seiffert SN, Hilty M, Perreten V, Endimiani A. Extended-spectrum cephalosporin-resistant gram-negative organisms in livestock: An emerging problem for human health? Drug Resist Updates. 2013; 16 (1-2): 22-45.
Ahmed KS, Feroz F, Noor R. Study of extended-spectrum b-lactamase-producing bacteria from urinary tract infections in Bangladesh. Tzu ChiMed J. 2013; 25(1): 39-42.
Schaeffer AJ. Potential role of phase variation of type I pilli in urinary tract infection and bacterial prostitutes. Infect Dis. 2005; 3:144-149.
Heidari M, Momtaz H, Madani M. Detection of the antibiotic resistance genes in Staphylococcus aureus isolated from human infections and bovine mastitis. African J Microbiol Res. 2011; 5(31): 5745-5749.
National Committee for Clinical Laboratory Standards (NCCLS): Methods for disk antimicrobial susceptibility tests for bacteria that grow aerobically. Approved standard M7-A5. Wayne, Pa: 2003
نخعی مقدم م، مشرفی ش. تعيين الگوي مقاومت آنتي بيوتيكي ايزوله‌هاي ادراري اشريشيا كلي و شيوع بتالاكتامازهاي وسيع طيف در بين آن¬ها. مجله ی دانشگاه علوم پزشكي و خدمات بهداشتي درماني سبزوار. 1388: (4)16: 228-223.
Akbari-Nakhjavani F, Mirsalehi A, HamidianM, Kazemi B, Mirafshar M, Jabal Ameli F. Antimicrobial susceptibility testing of Escherichiacoli strains isolated from urinary tract infections tofluoroquinolones and detection of gyrA mutationsin resistant strains. Daru. 2007; 15(2): 94-99.
Soleimani-Asl Y, Zibaei M, Firoozeh F. Detection of qnrA gene among quinolone-resistant Escherichia coli isolated from urinary tract infections in Khorram Abad during. 2011-2012; Feyz. 17(5): 488-495.
Muhammad I, Uzma M, Yasmin B, Mehmood Q, Habib B. Prevalence of antimicrobial resistance and integrons in Escherichia coli from Punjab, Pakistan. Braz J Microbiol. 2011; 42(3): 462-466.
Moreno E, Prats G, Sabate M, Perez T, Johnson JR, Andreu A. Quinolone, fluoroquinolone and trimethoprim/sulfamethoxazole resistance in relation to virulence determinants and phylogenetic background among uropathogenic Escherichia coli. Antimicrob Agents Chemother. 2006; 57(2): 204-211.
Bean DC, Livemore D, Hall LM. E .coli implications for Plasmids imparting sulfonamide resistance in persistence. Antimicrob Agents Chemother. 2009; 53 (4): 1088-1093.
Norouzi J, Akhavan sepahy A, Bazzazzadeh N. Detection of Sul2 Gene in Escherichia coli Isolated from Patients with Urinary Tract Infections Admitted to Clinical Centers of Khoy City. Zanjan Univ Med Sci. 2013; 21(88): 76-83.
Hammerum A, Sandvaye D, Andersen SR. Detection of sul1, sul2, sul3, in sulfonamide resistant Escherichia coli isolates obtained from healthy humans pork and pigs in Denmark. Int J Food Microbiol. 2006; 106 (4): 235-239.
Koljalg S, Trusaluk K, Vainumae I, Stsepetova J, Mikelsaar M. Presistance of Escherichia coli colones and phenotype and genotypic antibiotic resistance in recurrent urinary tract infections in childhood. J Clin microbial. 2009; 47 (3): 99-105.
Al-Agamy M. Molecular resistance mechanisms to older antimicrobial agents in Escherichia coli isolates. J African Microbiol.2012; 6: 106-111.