Isolation and biotyping of Yersinia enterocolitica isolated from chicken meat

Subject Areas : microbiology

Manouchehr Momeni-Shahraki ^{1 *}

1 -

Received: 2025-08-13 Accepted : 2025-09-24 Published : 2025-09-24

Keywords: Bio typing, Meat, Virulence genes. Yersinia,

Abstract :

Yersinia enterocolitica belongs to the family Enterobacteriaceae, which is known to be highly heterogenic andis divided into several biotypes and serotypes. Since the 1960s, this bacterium has increasingly been identified as an important foodborne pathogen. In this study, virulence factors such as yadA, inv, ail, ystA and virF of 65 strains isolated from chicken meat samples were analysed by the multiplex polymerase chain reaction (PCR) method. Of 65 isolates of Y. enterocolitica  biotyping revealed biotypes 1A (35.38%), 1B (26.15%), 2 (6.15%), 3 (9.23%), 4 (6.15%) and unclassified (16/94%). Polymerase chain reaction tests showed that the prevalence of virulent genes was 44.74% for yadA, 100% for inv, 50% for ail, 51.85% for ystAand35.18% for virF. This study highlighted the importance of chicken meat as a potential source of Y. enterocolitica infection in Iran.

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