List of Articles محمود شوندی

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  1 - Isolation and identification of sulfur-oxidizing chemolithotrophic bacteria from Qeshm salt cave
  Nina Zamani Mohammad Ali Amoozegar Maliheh Mehrshad Mahboobeh Darabi Seyed Abolhasan Shahzadeh Fazeli Mahmood Shavandi
  Background & Objectives: Chemolithotrophic bacteria have an important role in the biogeochemical cycle in natural ecosystems. For instance, the oxidative part of the sulfur cycle is handled by sulfur-oxidizing bacteria. Moreover, these bacteria have an important rol More

  Background & Objectives: Chemolithotrophic bacteria have an important role in the biogeochemical cycle in natural ecosystems. For instance, the oxidative part of the sulfur cycle is handled by sulfur-oxidizing bacteria. Moreover, these bacteria have an important role in various industrial fields including bioleaching. The aim of this study was isolation and identification of chemolithotrophic sulfur-oxidizing bacteria from the Namakdan cave Qeshm, Iran. Materials & Methods: In this cross-sectional study, after sampling from the Namakdan cave in Qeshm and transferring the samples (salt, water, and sediment) to the lab, sample inoculation into enrichment media was carried out with three concentrations of NaCl. Following pH and temperature measurement, samples were transferred into solid and two-phase media. Further characterization of the isolates was performed using 16S rRNA gene amplification. Further analysis was performed to characterize the autotrophic or heterotrophic features of the isolates. Biomass was examined using classical turbidity method. Results: Totally, 39 strains were isolated from samples, based on differences in primary features such as colony form or growth rate. A total of 5 isolates were selected for further studies. Phylogenetic analysis showed that these isolates belong to Halothiobacillus genus. Conclusion: Due to 30% salinity and neutral pH of this cave, isolation of chemolithotrophic sulfur-oxidizing bacteria was limited to few genera. Chemolithotrophic bacteria have longer growth time in comparison to heterotrophic ones, increasing generation time to 30 days. Manuscript profile
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  2 - Molecular diversity of heterotrophic bacteria and archaea of Namakdan Cave in Qeshm
  Mahboobeh Darabi Mohammad Ali Amoozegar Maliheh Mehrshad Nina Zamani Seyed Abolhasan Shahzadeh Fazeli Mahmood Shavandi
  20.1001.1.20083068.1397.11.34.5.7
  Background & Objectives: Given the high diversity, biotechnological applications and the effective role of bacteria in making and maintaining the ecosystem balance; biodiversity research are very important. Meanwhile, the halophilic bacteria and archaea have been co More

  Background & Objectives: Given the high diversity, biotechnological applications and the effective role of bacteria in making and maintaining the ecosystem balance; biodiversity research are very important. Meanwhile, the halophilic bacteria and archaea have been considered because of their biotechnological importance and specific ecological condition. In this study, we investigated the diversity of heterotrophic bacteria and archaea of Namakdan cave in Qeshm Island. Materials & Methods: This cross-sectional study was carried out by sampling from Qeshm Namakdan cave in November 2013. The diversity of the cave heterotrophic aerobic bacteria was analyzed using the culture method. Halophilic and halotolerant bacteria and Archaea under aerobic conditions were isolated by MGM and Marine agar media, respectively. Isolates were separated according to morphological differences, and primary biochemical features. Finally, 16s rRNA sequencing was performed for 32 isolates.Results: Among 172 isolates 16S rRNA sequencing was carried out for 27 strains. Phylogenetic analysis placed archaea in the euryarchaeota division and Halococcus, Haloferax, Haloarcula, Halogeometricum genus branches and bacteria in Firmicutes and Bacteroides divisions and in Aliifodinibius, Paenibacillus, Aquibacillus, Paraliobacillus, and Bacillus genus branches. Among the sequenced isolates, 11 isolates showed less than 89.7% similarity to the standard species, which is considered as a borderline point to present new microbial species. Conclusion: Placing the identified isolates in different phylogenetic divisions and genus branches demonstrates the wide microbial diversity of Qeshm Namakdan cave ecosystem. Presenting native microorganisms in new species and genera from unique ecosystems by introducing new genetic content provides access to new native genes and pathways. Manuscript profile
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  3 - Bacterial community structure in saline sediments from hypersaline wetland in south of Halghe Dare hills, Alborz province
  Seyed Sina Seyedpour Layalestani Mahmoud Shavandi Azam Haddadi Mohammad Ali Amoozegar Seyed Mohammad Mehdi Dastgheib
  20.1001.1.20083068.1399.13.0.2.0
  Background & Objectives: Survey of bacterial community structure in hypersaline ecosystems and identification of novel halophilic species can be very important from biotechnological and ecological aspects. In this study, we survey bacterial community structure in se More

  Background & Objectives: Survey of bacterial community structure in hypersaline ecosystems and identification of novel halophilic species can be very important from biotechnological and ecological aspects. In this study, we survey bacterial community structure in sediments from saline wetland in south of Halghe Dare hills as one of the hypersaline ecosystems in Alborz province. Materials & Methods: This cross-sectional study was performed by sampling from saline wetland in south of Halghe Dare hills in June 2018. Isolation of heterotrophic bacteria was conducted using R2A agar medium. After differentiation of isolates based on morphological and biochemical characteristics, identification and phylogenetic relationships analysis of selected isolates were performed by 16S rRNA gene sequencing and analysis using NCBI databases and bioinformatics softwares. The Illumina next-generation sequencing was also applied to survey bacterial diversity by cultivation-independent method. Results: Isolates included 13 species belonging to 8 genera including Bacillus (31.25%), Halomonas 25%, Gracilibacillus (12.50%), Virgibacillus (6.25%), Streptomyces (6.25%), Nitratireductor (6.25%), staphylococcus (6.25%) and Planococcus (6.25%). Illumina sequencing showed that Aneurinibacillus migulanus and Paenibacillus polymyxa were dominant species insoil sample. Conclusion: The results showed that the microbial population of the studied wetland is similar to the community of the wetlands reported in other parts of the world and dominated by halotolerant and halophilic species. Presence of various bacterial species and some probable novel taxonomic groups in saline wetland in south of Halghe Dare hills presents a new genetic and microbial source for future studies.   Manuscript profile
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  4 - The effects of pesticides on microbial population of saline soils using culture-dependent and next generation sequencing methods
  Safoura Hashemi joker Mahmoud Shavandi Azam Haddadi Ebrahim Alaie
  10.30495/jmw.2021.690437
  20.1001.1.20083068.1400.14.46.2.9
  Background & Objectives: Despite of widely use of pesticides in agricultural industry, their ecotoxicological impacts on the environment are not still well understood. In the present study, the effects of chlorpyrifos and deltamethrin pesticides on soil microbial di More

  Background & Objectives: Despite of widely use of pesticides in agricultural industry, their ecotoxicological impacts on the environment are not still well understood. In the present study, the effects of chlorpyrifos and deltamethrin pesticides on soil microbial diversity in different salinities were investigated.Materials and methods: Four soil samples with various salinities (0, 1%, 2% and 4%) were contaminated with 200 mg/kg of chlorpyrifos and deltamethrin. Then, the effects of these compounds were investigated by culture-dependent and Next Generation Sequencing (NGS) methods. The dominant bacterial strains were isolated and compared with the results of the NGS method.Results: Remarkable groups of active soil species were destroyed. The main groups at the phylum level were, Firmicutes, Proteobacteria, Bacteroidetes and Actinobacteria. Examination of the   pattern of operational taxonomic unit (OTU) showed that the lowest abundance belonged to the group of soil samples contaminated with chlorpyrifos and the highest abundance belonged to the non-contaminated sample. Alpha diversity analysis revealed that the highest number of strains was observed in non-saline and uncontaminated soil microcosms, and the lowest number of strains was observed in the presence of deltamethrin (1% salinity) and chlorpyrifos (0% salinity).Conclusion: The results indicated that soil salinity had significant impact on the resistance of soil microorganisms to the chlorpyrifos and deltamethrin. Every soil samples will have its own microbial composition based on its physicochemical conditions under the influence of pesticide contamination. By comparison of microbial composition by culture-dependent and molecular-based methods, almost similar findings were obtained.  Manuscript profile
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  5 - The effect of altitude on the bacterial diversity and abundance of the soil samples of Kazem Khan Mountain of saline Lake Urmia
  Fatemeh ghafarnejad mogadam Mahmoud Shavandi Azam Haddadi Mohammad Ali Amoozegar
  10.30495/jmw.2022.1966410.2033
  20.1001.1.20083068.1401.15.53.3.1
  Background & Objectives: Considering the critical conditions of Lake Urmia, identifying bacteria with the ability to live in extreme environments is valuable in terms of microbial applications and tolerance of the existing biological conditions, and it helps us to b More

  Background & Objectives: Considering the critical conditions of Lake Urmia, identifying bacteria with the ability to live in extreme environments is valuable in terms of microbial applications and tolerance of the existing biological conditions, and it helps us to better understand the surrounding environment. In this study, the most abundant microbial branch in the soil samples obtained from three different heights of 10, 150 and 250 meters of Qale Kazem Khan Mountain, which is located on the shore of a very salty lake in Urmia, has been investigated. Materials & Methods: The soil samples were collected to identify and classify Proteobacteria    subgroups using 16S rRNA sequencing using Next Generation Sequencing (NGS) as well as FLASH genetic software and UCHIME algorithm to identify the obtained sequences. Results: Altitude change affects the abundance of Alphaproteobacteria and Betaproteobacteria. The abundance percentage of Alphaproteobacteria has a direct relationship with altitude, while the abundance percentage of Betaproteobacteria increased with the decrease in altitude. Conclusion: in the overview the percentage of Proteobacteria abundance the samples has an inverse relationship with the increase in height, whereas in the separate examination of the microbial groups, a significant relationship between the increase and decrease in abundance and the height of sampling is observed. Also, two unknown and unclassified genera in the  Deltaproteobacteria order were also identified which a very high frequency percentage (18-27%) among the data had related to the three samples.   Manuscript profile