طاهر نژادستاری Articles List

List of Articles طاهر نژادستاری

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1 - Isolation and phylogenetic analysis of indigenous oil-degrading bacteria from soil of Karoon area in Ahvaz
Mohammad Hossein Arash Assadirad Mahnaz Mazaher Asadi Hamid Rashedi Taher Nejadsattari

  Background & Objectives: Bioremediation is the promising technology for the treatment of the contaminated sites since it is cost-effective and will lead to complete mineralization. This study was aimed to isolation and phylogenetic identification of indigeno More

  Background & Objectives: Bioremediation is the promising technology for the treatment of the contaminated sites since it is cost-effective and will lead to complete mineralization. This study was aimed to isolation and phylogenetic identification of indigenous oil-degrading bacteria from soil of Karoon area in Ahvaz. Materials & Methods: The crude oil contaminated soil of Karoon area in Ahvaz was sampled accidentally and under sterile condition. The amount of absorbable phosphorus was determined using Olson method and also, carbon, hydrogen and nitrogen by CHN meter device. Mineral salt medium containing 2% crude oil was used for isolation of oil eating bacteria. Following sieving the soil samples, the total carbon content of the soils were analysed by gas chromatography. Biochemical tests and PCR method were used to identify the dominant bacteria. Results: In this study, 44 bacterial strains were isolated, among them 20 isolates in the first and one in the second screening methods were selected, which was nominated as S31.This strain belonged to Bacillus licheniformis. The growth of the selected isolate in the media with 2% crude oil was better than the standard strain and remediated 84% of the crude oil in 30 days incubation time at about 30o C. Conclusion: The selected Bacillus could  use 2% of crude oil as source of carbon and energy and we suggest further studies on this bacterium in bacterial consortia. Manuscript profile
Open Access Article
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2 - The analysis of morphological acclimation of cyanobacterium Leptolyngbya sp. Isc25 to monochromatic red, green and blue light rays.
Ehteram Deylami Taher Nejadsattari Younes Ghasemi Shadman Shokravi

Background & Objectives: Due to pleomorphic feature of Cyanobacteria in different conditions, confirmation of their identification required several phytochemical, physiologic, molecular and genetic analysis after preliminary morphological analysis.  This study More

Background & Objectives: Due to pleomorphic feature of Cyanobacteria in different conditions, confirmation of their identification required several phytochemical, physiologic, molecular and genetic analysis after preliminary morphological analysis.  This study is the first report in Iran in which was aimed to identify Cyanobacteria Leptolyngbya sp. Isc25 based on molecular approaches and to analyze their morphologic response to monochromatic red, green and blue light rays. Material & Methods: In order to investigate the morphologic responses of Leptolyngbya sp. Isc25, the purified isolate of this Cyanobacterium in BG11 media were exposed first to autotrophic conditions (white light) and then to to monochromatic red, green and blue light rays. The morphological responses, flock production, structural changes, biometry and situation of trichome of Leptolyngbya sp. were evaluated using light microscopy and scanning electron microscopy (SEM) at different time periods, daily,  weekly and monthly. Results: Based on the morphologic and biometric analysis of the samples using light and scanning electron microscopy, the morphology and growth factor of this Cyanobacteria was affected by different environmental factors, and the best adaptation is obtained in green and red lights in comparison to blue light. Leptolyngbya sp. produced special cells with red pigments in their wall in case of exposure to green and red lights, which confirmed the variability of this species. Conclusion: The results show that Leptolyngbya sp. has morphological diversity and response differently in each light condition. Manuscript profile

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List of Articles طاهر نژادستاری