The analysis of morphological acclimation of cyanobacterium Leptolyngbya sp. Isc25 to monochromatic red, green and blue light rays.

Subject Areas : Microbial Biotechnology

Ehteram Deylami ¹ , Taher Nejadsattari ² , Younes Ghasemi ³ , Shadman Shokravi ⁴

1 - Ph. D. Student, Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
2 - Associate Professor, Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
3 - Professor, Department of Pharmaceutical Biotechnology, Shiraz University, Shiraz, Iran.
4 - Associate Professor, Department of Biology, Islamic Azad University, Gorgan, Iran.

Received: 2013-11-01 Accepted : 2014-01-23 Published : 2014-11-01

Keywords: Leptolyngbya sp, Morphological acclimation, Monochromatic light rays, Biometry,

Abstract :

Background & Objectives: Due to pleomorphic feature of Cyanobacteria in different conditions, confirmation of their identification required several phytochemical, physiologic, molecular and genetic analysis after preliminary morphological analysis.  This study is the first report in Iran in which was aimed to identify Cyanobacteria Leptolyngbya sp. Isc25 based on molecular approaches and to analyze their morphologic response to monochromatic red, green and blue light rays. Material & Methods: In order to investigate the morphologic responses of Leptolyngbya sp. Isc25, the purified isolate of this Cyanobacterium in BG11 media were exposed first to autotrophic conditions (white light) and then to to monochromatic red, green and blue light rays. The morphological responses, flock production, structural changes, biometry and situation of trichome of Leptolyngbya sp. were evaluated using light microscopy and scanning electron microscopy (SEM) at different time periods, daily,  weekly and monthly. Results: Based on the morphologic and biometric analysis of the samples using light and scanning electron microscopy, the morphology and growth factor of this Cyanobacteria was affected by different environmental factors, and the best adaptation is obtained in green and red lights in comparison to blue light. Leptolyngbya sp. produced special cells with red pigments in their wall in case of exposure to green and red lights, which confirmed the variability of this species. Conclusion: The results show that Leptolyngbya sp. has morphological diversity and response differently in each light condition.