Mohammad Hassan Shahhosseiny Articles List

List of Articles Mohammad Hassan Shahhosseiny

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1 - Presence of clade D Zooxanthellae in dominant Sclerectinian and soft corals in Larak island, Persian Gulf
Mohammad Hassan Shahhosseiny Pargol Ghavam Mostafavi Gholamhossein Vosughi Sanaz Azadbody

Abstract                         Background and Objectives: Dominant sclerectinian and soft corals contain the symbiotic single cell called zooxanthel More

Abstract                         Background and Objectives: Dominant sclerectinian and soft corals contain the symbiotic single cell called zooxanthellae which have important role in preparing organic material for coral requirements. The coral reefs of this area in the gulf are always under effects of environmental conditions, such as subtropical latitude, temperature and salinity variations, changing symbiotic zooxanthellae. The aims of this study was identification of Symbiodinium clades and presence of clade D in sclerectinian and soft corals of Larak island by molecular methods. Materials and Methods: Three soft coral species and five sclerectinian coral species were collected from north and north east of Larak island, respectively. After DNA extraction, partial 28S ribosomal DNA of Symbidinium were amplified by polymerase chain reaction (PCR). Then PCR products were analyzed by phylogenetic analyses of the partial 28S ribosomal sequence. Results: To follow amplification of 28S large ribosomal subunit gene, the 780 bp PCR products were sequenced and were compared to the gene bank. The results showed that all the symbiotic clades of soft corals in Larak Island belonged to clade D. Conclusion: Dominance of clade D in sclerectinian and soft corals in Larak island due to of high temperature in the Persian Gulf and unstable condition of Hormoz strait is normal and natural. Manuscript profile
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2 - Design of an internal control (IC) for molecular diagnosis of Mycobacterium tuberculosis
Mohammad Hassan Shahhosseini Maryam Ghahri Elham Moslemi

Background & Objectives: Tuberculosis is the second reason of deaths caused by known infectious agents. Although molecular approaches, such as PCR, are suitable techniques for detection of Mycobacterium tuberculosis (MTB), these techniques suffer of variable results More

Background & Objectives: Tuberculosis is the second reason of deaths caused by known infectious agents. Although molecular approaches, such as PCR, are suitable techniques for detection of Mycobacterium tuberculosis (MTB), these techniques suffer of variable results in different laboratory conditions. This study aimed to design, manufacture and apply an internal PCR control used for detection of MTB. Materials & Methods: To produce special MTB internal control, first individual PCR test primers was optimized for molecular detection of MTB, and then their sensitivity and specificity were measured. The composite primer for IC-MTB was also designed, replicated and colonized. The amplified IC-MTB was ligated to pTZ57R plasmid and was transformed into E. coli JM107. The minimum IC number in each PCR reaction was studied using dilution and PCR response spectrum with IC.  Results: The size of MTB diagnostic products with individual primers and IC-MTB were was 245 bp and 660 bp, respectively, which is a suitable difference in the size aspect. Minimum IC number was identified 1000 for each reaction. Minimum and maximum sensitivity PCR test by IC for MTB DNA was determined 10 and 10 million bacteria, respectively. No unwanted products were observed in characteristic tests by different agents. Conclusion: Using an internal control as an inside control system we could detect the errors in MTB molecular diagnosis test. In fact, IC amplification is representative of correct procedure in amplification and detection steps. Manuscript profile
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3 - Loop Mediated Isothermal Amplification of Hepatitis B Virus in Hemodialysis Patients
Mohmmad hasan Shahhosseiny Zohre Esmailii Elham Moslemi Parichehre Yaghmaii

Background and Objectives: Infections with Hepatitis B virus in hemodialysis centers still remain as a major problem. The propose of this study is optimization and development of LAMP technique for assessment of HBV infection in serum of hemodialysis patients. Material More

Background and Objectives: Infections with Hepatitis B virus in hemodialysis centers still remain as a major problem. The propose of this study is optimization and development of LAMP technique for assessment of HBV infection in serum of hemodialysis patients. Material and Methods: This cross sectional study was performed on totally, 136 serum samples were obtained from the patients how referred to hemodyalis center in Tehran as: 52 serum samples from Mastafa khomaini hospital, 54 serum samples from Chamran hospital and 31 serum samples from Erfan hospital. After DNA extraction, 6 HBsAg specific primers were used for optimizing of reaction conditions and material concentration for LAMP. LAMP products were evaluated by adding 1% SYBER Green and electrophoresis stained by Ethidium-Bromide. Results: LAMP sensitivity was determined as 4 particles in this study. In addition, LAMP technique Showed a high specify for detecting the viruses. Totally, 13and 7 cases of the serum samples of Mastafa khomaini were positive by LAMP and PCR respectfully. Also, 4 and 2 samples for second group and 2 and 2 samples for third group were positive respectfully by LAMP and PCR. Conclusion: The LAMP technique is a more sensitive and cost friendly system to detect HBV in comparison to conventional PCR. Manuscript profile
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4 - Assessment of the survival rate of non-cultivable forms of Helicobacter pylori in water samples
Parasto Chamanrokh Mohammad Hassan Shah Hosseini Mahnaz Mazaheri Asadi Taher Nejad Sattari Davood Esmaeili

H. Pylori is an important cause of human peptic ulcers and stomach cancers. Once the bacterium is placed in the water, it changes into a viable, but non-cultivable coccoid form, which is considered as an important factor to spread out the infection. The aim of this stud More

H. Pylori is an important cause of human peptic ulcers and stomach cancers. Once the bacterium is placed in the water, it changes into a viable, but non-cultivable coccoid form, which is considered as an important factor to spread out the infection. The aim of this study was to evaluate the survival rate of coccoid forms of H. pylori in water samples, using PCR and culture methods. This experimental study was performed on 10 strains of H. pylori isolated from clinical specimens. Isolates were added to water at the temperatures of 4°C, 22°C, and 37°C and incubated at intervals of 1 and 2 months. Each time, the samples were cultured on Brucella blood Agar medium. Following DNA extraction, the presence of glmM gene was confirmed using PCR method. Furthermore, the sensitivity and specificity of PCR method were evaluated. While culturing in first month at 4°C showed no H. pylori coccoid growth on medium, some positive results (growth rate of 10% and 20%, respectively) were detected at 22°C and 37°C during the same month. No positive result was obtained during the second month. Performing PCR, with more sensitivity as compared to culturing method, identified H. pylori coccoid growth of 10%, 30%, and 40%, for the first month, and 0%, 20%, and 30% for the second month at 4°C, 22°C, and 37°C, respectively. The results of this study showed that the non-cultivable cocoid forms of H. pylori in water can be detected by non-culturing methods such as PCR which is sensitive, specific, and accurate. Manuscript profile

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List of Articles Mohammad Hassan Shahhosseiny