Loop Mediated Isothermal Amplification of Hepatitis B Virus in Hemodialysis Patients

Subject Areas : Molecular Microbiology

Mohmmad hasan  Shahhosseiny ¹ (Department of Microbiology, Shahr -e- Qods branch, Islamic Azad University, Shahr -e- Qods, Iran)
Zohre  Esmailii ² (Department of Biology, Science and Research branch, Islamic Azad University, Tehran, Iran)
Elham  Moslemi ³ (Department of Biology, East-Tehran branch, Islamic Azad University, Tehran, Iran)
Parichehre  Yaghmaii ⁴ (Department of Biology, Science and Research branch, Islamic Azad University, Tehran, Iran)

Received: 2009-08-16 Accepted : 2009-10-17 Published : 2009-10-01

Keywords: PCR, Hemodialysis, LAMP, Hepatitis B,

Abstract :

Background and Objectives: Infections with Hepatitis B virus in hemodialysis centers still remain as a major problem. The propose of this study is optimization and development of LAMP technique for assessment of HBV infection in serum of hemodialysis patients. Material and Methods: This cross sectional study was performed on totally, 136 serum samples were obtained from the patients how referred to hemodyalis center in Tehran as: 52 serum samples from Mastafa khomaini hospital, 54 serum samples from Chamran hospital and 31 serum samples from Erfan hospital. After DNA extraction, 6 HBsAg specific primers were used for optimizing of reaction conditions and material concentration for LAMP. LAMP products were evaluated by adding 1% SYBER Green and electrophoresis stained by Ethidium-Bromide. Results: LAMP sensitivity was determined as 4 particles in this study. In addition, LAMP technique Showed a high specify for detecting the viruses. Totally, 13and 7 cases of the serum samples of Mastafa khomaini were positive by LAMP and PCR respectfully. Also, 4 and 2 samples for second group and 2 and 2 samples for third group were positive respectfully by LAMP and PCR. Conclusion: The LAMP technique is a more sensitive and cost friendly system to detect HBV in comparison to conventional PCR.

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