Molecular Identification of beta-lactamase genes (PER, VEB & GES)in Salmonella Enteritidis isolated from poultry meat by Multiplex-PCR

Subject Areas : Food Science and Technology

M. Esfandian ¹ , Gh. Moradli ²

1 - Department of Microbiology, Saveh Branch, Islamic Azad University, Saveh, Iran
2 - Assistant Professor, Department of Microbiology, Saveh Branch, Islamic Azad University, Saveh, Iran

Received: 2016-10-02 Accepted : 2018-05-25 Published : 2018-09-23

Keywords: blaVEB, blaPER, blaGES, SalmonellaEnteritidis,

Abstract :

Salmonellosis is an important disease in animals and human which is caused by different serovars of Salmonellaenterica. SerovarsEnteritidis is one of the most prevalent sorvars in animals and poultry and also is the food-borne pathogen. The aim of this study is to identify the prevalence of blaVEB, blaPER and blaGES in SalmonellaEnteritidis isolated from chicken samples.In this study, 60 Salmonella samples isolated from chickens were collected and confirmed as Salmonella by culture and biochemical tests. Serotyping was performed using O and H antisera. Multiplex-PCR was done for identification of ESBL genes including blaPER, blaVEB and blaGES. The serotyping results showed that all of the 60 samples belonged to group D and serovarEnteritidis. Among 60 samples, 3 had the blaPER, 2 had the blaVEb and 1harboured the blaGES gene.Antibiogram results showed the most resistance was to ceftazidime (64%) and the most susceptibility was to meropeneme and ceftizoxime (92%). As regards Salmonella is a food-borne bacteria, the presence of ESBL in food samples even in small numbers can be a serious alarm.

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