Investigating the degree of contamination of buffalo meat slaughtered in Urmia industrial slaughterhouse with Sarcocyst parasite using PCR method
Mahdieh Sadati ghale
1
(
Student in general Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran
)
S. Rasouli,
2
(
Department of Parasitology, Faculty of Veterinary medicine, Urmia Branch, IslamicAzad University, Urmia, Iran
)
Mohammad Reza Asgharzadeh
3
(
, Department of Biology, Faculty of Basic Science, Urmia Branch, Islamic Azad University, Urmia, Iran
)
Keywords: Sarcositis, Molecular Prevalence, 18s rRNA, Buffalo, Urmia,
Abstract :
Sarcocystis species are cyst-forming intracellular protozoan parasites with an obligate two-host life cycle between predators as main hosts and prey animals as intermediate hosts. The prevalence of infection with this protozoan parasite in domestic animals such as cows, buffalo, sheep, goats and camels in Iran has been high. This study was conducted in order to investigate the prevalence of Sarcocystis infection in slaughter buffaloes of Urmia city by molecular method (PCR). A total of 100 buffalo slaughtered in the slaughterhouse of Urmia city in 1402 were randomly selected and samples were taken from the muscles of the esophagus, diaphragm, heart, tongue, jaw, and intercostal muscles. The samples were examined macroscopically and molecularly using 18s rRNA gene.In the macroscopic examination, 5% of the samples obtained from buffaloes were infected with sarcocystis, while 12 samples (12%) of the tissue samples of slaughtered buffaloes of Urmia city had 18s rRNA sarcocytic gene. Statistical analysis showed that the prevalence of infection in old buffaloes was significantly higher than that of young calves, but gender had no significant effect on the prevalence of sarcosis in the studied buffaloes (p>0.05). The results of the present study indicate relatively high contamination in the buffalo populations of Urmia. Therefore, it is recommended to carry out preventive measures such as careful inspection of carcasses and complete or partial removal of carcasses in the slaughterhouse and to raise awareness to prevent the consumption of raw and undercooked meat
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