Study of the effect of glutamic acid on some physiological properties of stevia (Stevia rebaudiana) under salinity stress

Subject Areas : Environmental physiology

Parastoo Majidian ¹ , masoumeh salemi ² , Mahyar Gerami ³ , Masoud Azadbakht ⁴

1 - Crop and Horticultural Science Research Department, Mazandaran Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Sari, Iran
2 - 1Biology Department, Sana Institute of Higher Education, Sari, Iran
3 - Biology Department, Sana Institute of Higher Education, Sari, Iran
4 - Biology Department, Sana Institute of Higher Education, Sari, Iran

Received: 2023-02-12 Accepted : 2024-12-17

Keywords: antioxidant enzyme, Glutamic acid, Plant Tissue Culture, Salinity, Stevia,

Abstract :

Today, salt stress is known as one of the most important food security challenges in the world. Salinity stress has a transient effect on the growth and development of plant, leading to a decrease in growth and finally the death of plants. On the other hand, the stevia plant, as one of the useful medicinal plants, has faced an increase in production demand today. However, since this plant is relatively sensitive to salt stress, the use of compounds such as glutamic acid can greatly help plant growth in salty conditions. Therefore, in this study, we intend to investigate the effect of salt stress and glutamic acid in vitro. Examining the results and data of the salt stress level showed that the stevia plant is quite sensitive to the stress of 100 and 200 mM and is destroyed. But the treatment of glutamic acid prevented the death of plant and had a positive and significant effect on the growth of plant. A more detailed examination of the results showed that the treatment of 0.5 mg/L glutamic acid could completely neutralize the effect of 200 mM salt stress. Examining the measured traits showed that the plant was able to deal with the effects of salinity stress by using mechanisms such as catalase and guaiacol peroxidase antioxidant enzymes as well as proline. Also, the examination of morphological traits showed a significant increase in growth of all these traits under the treatment of 0.5 mg/liter. Finally, according to the results and data obtained from this research, applying the treatment of 0.5 mg/liter of glutamic acid was able to greatly reduce the effect of salinity stress and help the growth and development of plants in these difficult conditions.