Effect of different concentrations of beta-cyclodextrin nanoparticles on growth indexes and enzymatic defense systems, Ion leakage and amount of membrane lipid peroxidation in basil medicinal plant (Ocimum basilicum L. c.v. keshkeni luvelou)

Subject Areas : Genetic

azadeh loni ¹ , Sara Saadatmand ² , Hossein Lari Yazdi ³ , Alireza Iranbakhsh ⁴

1 - Department of Biology, Faculty of base Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 - Department of Biology, Faculty of base Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
3 - Department of Biology, Faculty of base Science and Research Branch, Islamic Azad University Boroujerd branch, Boroujerd Iran
4 - Department of Biology, Faculty of base Science, Science and Research Branch, Islamic Azad University, Tehran, Iran

Received: 2020-08-07 Accepted : 2020-10-19 Published : 2021-12-22

Keywords: nanoparticles, enzymes, Ion leakage, Growth index, Ocimum basilicum, beta-cyclodextrin,

Abstract :

By nanotechnology entrance into the field of medicinal plants, the agricultural industry and food industry ensures an increase in the amount and quality of their products, along with the preservation of the environment. The properties of materials are changed by changing their size to nano. The use of beta-cyclodextrin nanoparticles is described as a new protection strategy of the plant and induces a plant defense response. For this purpose, to study the effect of beta-cyclodextrin nanoparticles on the physiologic indexes and biochemical activities in basil of keshkeni luvelou cultivar, an experiment was performed based on a completely randomized design with four replications at four levels of 0.10, 50, 100mg/l in the greenhouse. The results showed that different growth indices such as root and stem length and diameter, fresh and dry weight of roots, shoots, and leaf area increased by 5% compared to the control and the highest increase was reported at 50ppm. Data analysis showed that the effect of different treatments on the activity of superoxide dismutase and catalase, peroxidase enzymes, malondialdehyde content, and ion leakage of roots and leaves were significantly increased (P≤5%) compared to the control. Membrane stability index was assessed by measuring the electrolyte leakage of leaves and roots. A significant reduction (P≤5%) of ion leakage and malondialdehyde of roots and leaves was observed in the treatment of 50mg/l nano-beta cyclodextrin which indicates an increase in enzyme activity. With the entrance of nanoparticles into agriculture can minimize the scope of chemical control and disruption of environmental physiological practices. The trend of changes in physiological and biochemical parameters studied in the present study are relatively reliable indicators to introduce the best concentration of beta-cyclodextrin nanoparticles for basil.

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