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Open Access Article
1 - Effect of different concentrations of beta-cyclodextrin nanoparticles on growth indexes and enzymatic defense systems, Ion leakage and amount of membrane lipid peroxidation in basil medicinal plant (Ocimum basilicum L. c.v. keshkeni luvelou)
azadeh loni Sara Saadatmand Hossein Lari Yazdi Alireza IranbakhshBy nanotechnology entrance into the field of medicinal plants, the agricultural industry and food industry ensures an increase in the amount and quality of their products, along with the preservation of the environment. The properties of materials are changed by changin MoreBy nanotechnology entrance into the field of medicinal plants, the agricultural industry and food industry ensures an increase in the amount and quality of their products, along with the preservation of the environment. The properties of materials are changed by changing their size to nano. The use of beta-cyclodextrin nanoparticles is described as a new protection strategy of the plant and induces a plant defense response. For this purpose, to study the effect of beta-cyclodextrin nanoparticles on the physiologic indexes and biochemical activities in basil of keshkeni luvelou cultivar, an experiment was performed based on a completely randomized design with four replications at four levels of 0.10, 50, 100mg/l in the greenhouse. The results showed that different growth indices such as root and stem length and diameter, fresh and dry weight of roots, shoots, and leaf area increased by 5% compared to the control and the highest increase was reported at 50ppm. Data analysis showed that the effect of different treatments on the activity of superoxide dismutase and catalase, peroxidase enzymes, malondialdehyde content, and ion leakage of roots and leaves were significantly increased (P≤5%) compared to the control. Membrane stability index was assessed by measuring the electrolyte leakage of leaves and roots. A significant reduction (P≤5%) of ion leakage and malondialdehyde of roots and leaves was observed in the treatment of 50mg/l nano-beta cyclodextrin which indicates an increase in enzyme activity. With the entrance of nanoparticles into agriculture can minimize the scope of chemical control and disruption of environmental physiological practices. The trend of changes in physiological and biochemical parameters studied in the present study are relatively reliable indicators to introduce the best concentration of beta-cyclodextrin nanoparticles for basil. Manuscript profile -
Open Access Article
2 - Interaction of salinity and chromium on pigments and photosystem II function of Portulaca oleracea L.
Zahra Talebzadeh Rahele Rahbarian Mohabat Nadaf Hamid SobhanianThe aim of this study was to investigate the interaction of salinity and chromium stress on some morphological and physiological characteristics of portulaca oleracea. In a completely randomized design with 3 replications and 4 salinity levels (0, 4, 8 and 12 dS/m) usin MoreThe aim of this study was to investigate the interaction of salinity and chromium stress on some morphological and physiological characteristics of portulaca oleracea. In a completely randomized design with 3 replications and 4 salinity levels (0, 4, 8 and 12 dS/m) using sodium chloride every 4 days through irrigation and 5 levels of chromium (0, 7, 14, 21 and 28 mg/ml). Kg (dry weight of soil) experiment was performed and before sowing seeds in soil with increasing potassium dichromate to soil, different levels of chromium treatment were prepared. The values of ion leakage, fresh and dry weight and leaf area, chlorophyll a, b and total, chlorophyll stability coefficient, carotenoids, photosystem II efficiency and relative water content of leaves were measured after 60 days of planting. The results showed that with increasing different levels of chromium and salinity, a significant decreasing trend (P≤0.05) was observed in fresh and dry weight, leaf area, chlorophyll a, b and total, carotenoids, photosystem II efficiency. The lowest efficiency of photosystem II and the highest rate of ion leakage were observed at salinity levels of 12 dS/m and chromium 28 mg/kg. The interaction of salinity and chromium had a significant decreasing effect on chlorophyll content, fresh and dry weight and leaf area. Was alone. However, salinity and chromium reduced the growth of portulaca oleracea leaves. Manuscript profile -
Open Access Article
3 - The effect of salinity stress on the morphophysiological characteristics of some pomegranate genotypes
Sareh Sabahi Azam Jafari Ali Momenpour Mostafa ShirmardiPomegranate is one of the most important horticultural products that cultivated in many tropical and subtropical parts of the world. Meanwhile, in some pomegranate orchards, salinity stress is one of the abiotic stresses that harm agricultural crops such as pomegranate MorePomegranate is one of the most important horticultural products that cultivated in many tropical and subtropical parts of the world. Meanwhile, in some pomegranate orchards, salinity stress is one of the abiotic stresses that harm agricultural crops such as pomegranate production by reducing productivity. This research aims to compare growth characteristics between selected genotypes including 1- Chah Afzal 2- Vahshi Babolsar, 3- Narak Lasjerd Semnan 4- Poost Siahe Yazd, 5- Malase Yazdi, 6- Rababe Neyriz, under salinity stress, in a randomized complete block design with three replicaions at Chah Afzal research station of Ardakan city. During the whole experiment, the trees were irrigated with saline water with salinity of 9 ± 0.5 dS/m every 12 days. Based on the results of this research and considering the lowest amount of ion leakage, the highest content of relative humidity and potassium, the high amount of total chlorophyll, carotenoid and leaf surface ratio, the lowest sodium to potassium ratio and necrosis percentage Chah Afzal genotype showed very good resistance to salinity conditions, followed by Poost Siahe Yazd, Vahshi Babolsar, Narak Lasjerd, Malase Yazdi and finally Rababe Neyriz were highly sensitive to salinity. According to the results of cluster analysis, the highest similarity between genotypes based on the parameters mentioned above was between the Narak Lasjerd and Malase Yazdi. The highest difference between Chah Afzal genotype and other genotypes was registered. This shows a significant difference in the superiority of Chah Afzal genotype over other genotypes in total traits. Therefore, due to the compatibility and proper growth of these genotypes in saline conditions, it is suggested that they could be used as a rootstock and the cvs. Malase Yazdi and Rababe Neyriz, which also produced flowers and fruits in saline conditions, can be grafted on them. Manuscript profile -
Open Access Article
4 - Effect of salicylic acid foliar application on flowering and growth characteristics of lantana (Lantana camara Linn.) under salinity stress
Maryam Dehestani-Ardakani Parisa Ghatei Ali Momenpour Jalal Gholamnezhad Zahra FakharipourLantana camara Linn. is a flowering ornamental plant belonging to family Verbenaceae. The aim of the present study was to investigate the effect of salicylic acid foliar application on flowering improvement and growth characteristics of lantana under salinity stress. In MoreLantana camara Linn. is a flowering ornamental plant belonging to family Verbenaceae. The aim of the present study was to investigate the effect of salicylic acid foliar application on flowering improvement and growth characteristics of lantana under salinity stress. In a factorial experiment and completely randomized design (CRD), three levels of SA (0, 0.5 and 1mM) and five levels of salinity (0.5, 3, 5, 7 and 9 dS.m-1) were applied. The results indicated that increasing salinity levels from 0.5 to 9 dS.m-1 significantly reduced all studied growth parameters levels, chlorophyll contents and potassium uptake. While Na+uptake, Na+/K+ and ion leakage were increased relative to control. Also, the results indicated that the salicylic acid significantly increased plant growth and physiological traits. Application of salicylic acid (0.5 mM) improved vegetative, flowering growth and nutrient uptake under salt stress. At salinity level of 9 dS m-1, application of 1mM salicylic acid increased the height of the main branch and the number of flowers by3.22 and 2.14 times, respectively, compared to the control and decreased the sodium uptake by 2.46 times. At the same level of salinity, treatment of plants by 0.5mM salicylic acid increased plant height, stem diameter, increased diameter of main branch, number of flowers and fresh weight of flowers by 70.64%, 2.15, 2.14 and 5.8 times respectively, in compare to the control. Considering that the plants maintained their vegetative growth and flowering well up to salinity level of 7dS.m-1, it seems that the lantana is able to tolerate salinity. In general, It seems that under high salinity levels, salicylic acid (0.5 mM) was the most effective treatment for mitigating the deleterious effect of salt stress in lantana plants. Manuscript profile -
Open Access Article
5 - The Effect of Paclobutrazol on Physiological Characteristics of Bermuda Grass under Drought Stress
asghar pakdel حمید رضا میری رامین سامانی بابادائیGrass is considered as one of the most important covering plants in designing green spaces. We aimed to assess the growth retarding effects of Paclobutrazol on several physiological characteristics of Bermuda grass under dry stress in the form of a randomized factorial MoreGrass is considered as one of the most important covering plants in designing green spaces. We aimed to assess the growth retarding effects of Paclobutrazol on several physiological characteristics of Bermuda grass under dry stress in the form of a randomized factorial design study with three repetitions. The treatments in this study were paclobutrazol at concentrations of 0, 25, 50, and 75 mg/l and drought stress at four levels (irrigation based on 100, 80, 60, and 40% of the field’s capacity). We found that grass under severe drought stress conditions (40% FC) and were treated with concentrations of 50 and 75 mg/l Paclobutrazol had the highest amount of proline. Grass under moderate drought stress (60% FC) and was treated with Paclobutrazol 50 mg/l had the highest amount of soluble carbohydrates. When using 75 mg/l Paclobutrazol resulted in increased chlorophyll in Bermuda grass. Grass that was irrigated under normal conditions (100% FC) and was treated with different concentrations of paclobutrazol had the highest relative water content. The highest ion leakage was seen in grass under severe drought stress (40% FC) and 0 mg Paclobutrazol. In conclusion, we suggest the use of 50 mg/l Paclobutrazol in order to reduced water consumption or enhancing the growth of grass under drought stress. Manuscript profile -
Open Access Article
6 - Evaluation of Cell membrane Instability of Oily Sunflower Varieties under Water Deficit and Salicylic Acid Application
Mohammad Sibi Mohammad Mirzakhani Masoud Gomarian Hesamedin YaqobiExperimental treatments comprised of water stress as a major factor in three levels including I0 = irrigation water requirement of crop (control), I1= 80 percent of the amount of irrigation water requirements of crop, I2= 60 percent of the amount of irrigation water req MoreExperimental treatments comprised of water stress as a major factor in three levels including I0 = irrigation water requirement of crop (control), I1= 80 percent of the amount of irrigation water requirements of crop, I2= 60 percent of the amount of irrigation water requirements of crop in the main plots and different varieties of sunflower including V1= Azargol, V2= Alstar, V3= Ariflor and two levels of salicylic acid containing SA0= without salicylic acid and SA1= with salicylic acid (sprayed) as a minor factor in a factorial combination of sub-plots. The results showed the very significant effect (p<0.01) of different levels of water stress and varieties of sunflower on stem diameter, number of leaves, number of hollow grains, biological yield, ion leakage of cell (cell membrane instability), leaf water content, initial water content and ultimate water of leaf. Salicylic acid also had significant effect on number of leaves, cell ion leakage (cell membrane instability), leaf water content, initial water content and leaf ultimate water at a level of statistical significance, whereas stem diameter, number of hollow grains and biological yield did not affected by salicylic acid. Thus, ion leakage cell increased with increasing intensity of water deficit, therefore the highest average of ion leakage (2854.9 μs) obtained from extreme stress (60 percent of the amount of irrigation water requirements of crop). Manuscript profile -
Open Access Article
7 - Effect of Different Amounts and Application Number of Salicylic Acid on Increasing Drought Tolerance of Fig in Rain-fed Conditions of Estahban Region
Ramin Babadaei Samani Alireza Javid Majid ShaebaniToday, the use of salicylic acid as one of plant growth regulator substances is a common way to increase plant resistance to stresses such as drought. This experiment was conducted in order to determine the effects of different concentration and different application nu MoreToday, the use of salicylic acid as one of plant growth regulator substances is a common way to increase plant resistance to stresses such as drought. This experiment was conducted in order to determine the effects of different concentration and different application number of salicylic acid on some morphological and physiological characteristics of fig in rain-fed conditions of Estahban region. Investigation was performed as factorial based on a randomized complete block design (CRBD) with three replications. The studied factors included salicylic acid in four levels (0, 0.5, 1 and 1.5 mM) and different number of foliar sparying in three levels (one, two and three times in three week intervals). The first foliar application of salicylic acid was carried out three weeks after caprification of trees. The parameters such as chlorophyll, carotenoids, proline, ion leakage, average leaf area, shoot growth, relative water content (RWC) and yield were measured. The results showed that the use of salicylic acid increased chlorophyll a, chlorophyll b, total chlorophyll, carotenoids, relative water content, proline and yield, but, decreased ion leakage of the fig cv. "Sabz". Application of this compound did not significantly affect on shoot growth and leaf area. The effect of application number of salicylic acid on chlorophyll a, total chlorophyll and ion leakage was significant, but, had no significant effect on other traits. Generally, results indicated that application of salicylic acid significantly improved physiological characteristics and drought tolerance of fig in rain-fed conditions. Manuscript profile -
Open Access Article
8 - Response of Pistachio (Pistacia vera L.) Seedling Rootstocks to Salicylic Acid Foliar Application under Water Stress
Mostafa Ghasemi Kazem Arzani Abbas Yadollahi Hossein Hokmabadi Majid Agha-Alikhani -
Open Access Article
9 - Physiological, Biochemical, and Developmental Responses of some Pistachio Genotypes under Drought Stress
Mostafa Ghasemi Shiva Ghasemi Mehdi Mohammadi-Moghadam Saeid Kashanizadeh Mansoore ShamiliPistachio is one of the economic nut fruits in Iran. Water limitation is the most restrictive factor for its production. To overcome the water scarcity crisis, introducing drought-tolerant rootstocks is among the crucial breeding strategies. To investigate the drought t MorePistachio is one of the economic nut fruits in Iran. Water limitation is the most restrictive factor for its production. To overcome the water scarcity crisis, introducing drought-tolerant rootstocks is among the crucial breeding strategies. To investigate the drought tolerance of five Qazvin native pistachio genotypes, an experiment was carried out as a factorial experiment in a completely randomized design with four replications in the greenhouse conditions. The factors were pistachio genotypes (Madari, KalKhandan, Kalehbozi, Sefid, and Ghermez) and irrigation regime (normal conditions and drought stress). The highest relative water content under drought irrigation conditions belonged to the Sefid (59.99%), and Ghermez (59.09%) genotypes. The lowest value (54.68%) belonged to the Madari genotype. The highest electrolyte leakage under drought irrigation conditions belonged to the Madari genotype (55.75%). The lowest electrolyte leakage (42.44%) belonged to the Sefid genotype. Under drought stress, the highest amount of chlorophyll a (2.12 mg g-1 fresh weight), total chlorophyll (3.051 mg g-1 fresh weight), and carotenoid (2.38 mg g-1 fresh weight) was observed in Ghermez genotype. In contrast, the highest amount of chlorophyll b (1.34 mg g-1 fresh weight) was observed in the Sefid genotype. The lowest amounts of chlorophyll and carotenoid in the drought stress belonged to the Madari genotype. According to the results, the Ghermez and Sefid genotypes with lower electrolyte leakage and higher relative water content, chlorophyll, carotenoid, and biomass under water stress, were the more drought-tolerant genotypes. Madari and KalKhandan genotypes with higher electrolyte leakage and lower relative leaf water content, chlorophyll, and biomass were the most drought-sensitive genotypes. Manuscript profile -
Open Access Article
10 - Relationship Between Frost Injury and Ion Leakage as an Indicator of Cold Hardiness in 60 Almond Selections
A. Imani K. barzegar S. Piripireivatlou -
Open Access Article
11 - The effect of methanol foliar application on some physiological traits of cowpea bean (Vigna unguiculata L.) under drought stress conditions
ali akbar saneinejad Mahmood Tohidi Behnam Habibi Khaniani Mehdi Sadeghi Mohamad KhoramianThe aim of this study id analysis the effect of methanol foliar application on the physiological traits of beans genotype under water deficit stress as a split plot design was conducted in a randomized complete block design in Safiabad Dezful Agricultural Research and N MoreThe aim of this study id analysis the effect of methanol foliar application on the physiological traits of beans genotype under water deficit stress as a split plot design was conducted in a randomized complete block design in Safiabad Dezful Agricultural Research and Natural Resources and Center in 2018 and 2019 years. Water deficit stress was the main plots include four levels (25, 50, 75 and 100% of plant water requirement) and combined foliar aplication of methanol including 3 levels (control, 10 and 20%) and genotype treatment including two cultivars omidbakhsh 1057 And Mashhad was in sub-plots. The results showed that the interaction of water deficit stress and foliar application of methanol on ion leakage, chlorophyll a, carotenoids, leaf soluble carbohydrates and proline grains was significant at the 1% probability level. The lowest amount of ionic leakage observed under the interaction of 100% water deficit stress and 10% foliar aplication of methanol (25/23 μmos/cm) and The highest amount of ion leakage under the interaction of 25% water deficit stress and the without foliar aplication of methanol (control) (42.25 μmos/cm). The highest amount of chlorophyll a was observed in line 1057 under the 100% water deficit stress and 20% foliar aplication of methanol (0.19 µmol.g-1 FW). The highest concentration of carotenoids in line 1057 under the treatment of 100% water deficit stress (0.19 µmol.g-1 FW) and the highest amount of soluble carbohydrates under the treatment of 100% water deficit stress and 20% foliar aplication of methanol (50.83 µmol.g-1 FW) was observed. Manuscript profile
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