The effect of coenzyme Q10 on the prevention of testicular tissue damages in Alloxan-induced diabetic rats

Subject Areas : Veterinary Clinical Pathology

seyyed sajjad  hejazi ¹ (استادیار گروه علوم پایه، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران.)
ramin  jahangirfard ² (دستیار گروه علوم تشریحی، دانشکده دامپزشکی، دانشگاه ارومیه، ارومیه، ایران.)

Received: 2015-07-21 Accepted : 2015-10-19 Published : 2015-08-23

Keywords: Q10, Testis, Rat, Alloxan,

Abstract :

Coenzyme Q10 is considered as a vitamin or a vitamin-like substance which is found in food resources naturally like other vitamins. In recent years, several drugs have been used effectively for treatment of diabetes, but the treatment or protective effect of few of them have been studied microscopically. In the present study, the complementary effect of Q10 on testicular structure in alloxan-induced diabetic rats was studied. Forty male Wistar rats were randomly divided into four groups of 10 rats. The first group was the control group; the second group was the control group treated with 75 mg/kg Q10 for one month, orally (gavage); the third one was the alloxan-induced diabetic treatment group in which a single dosage of 120 mg/kg alloxan was administered intra peritoneally, and the fourth group was the diabetic treatment group treated with Q10. The rats’ weight, testis weight, seminiferous tubule diameter, testicular capsule thickness and seminiferous tubular epithelium thickness were measured. Statistical analysis was performed using ANOVA and Duncan,s multiple range test. Q10 demonstrated a modifying effect on the diabetic rats’ testicular damage such that damages in tissue structure and the sex cells, as well as shrinkage of the seminiferous tubules were observed very slightly. Furthermore, Q10 could inhibit the infiltration of the vessels into and under the capsule space which was significant in diabetic group. The use of Q10 as an antioxidant significantly protected the spermatogenic cells, testicular capsule, testis weight, and seminiferous tubule diameter from the damage of diabetes in rats.

References:

ایوبی، ع.، ولی زاده، ر.، آرشامی، ج. و موسوی، ز. (1393). تأثیر عصاره آبی-الکلی صمغ آنغوزه بر وزن بدن و بیضه، هورمون تستسترون  و اسپرماتوژنز در رت­های بالغ ویستار. نشریه پژوهشهای علوم دامی ایران، دوره 6، شماره 2، صفحات: 180-178.
حاتمی، ل. و  استخر، ج. (1392). تاثیر عصاره آبی الکلی گیاه بابونه آلمانی بر محور هورمونی هیپوفیز-گناد و
تغییرات بافتی بیضه در موش صحرایی نر بالغ. مجله دانشگاه علوم پزشکی فسا، دوره 3، شماره 1، صفحات: 62-56.
عرفانی، ن.، بهرامی، م. و مروتی، ح. (1392). مطالعه اثر محافظتی آلوئه ورا بر تغییرات هیستولوژیک و هیستومتریک بیضه موش صحرائی دیابتی. مجله دامپزشکی ایران، دوره 9، شماره 39 ، صفحات: 87-78.
عمواوغلی تبریزی، ب. و مهاجری، د. (1390). تاثیر عصاره الکلی ریشه شلغم بر نفروپاتی پیشرس دیابتی در موش‌های صحرایی. مجله تحقیقات علوم پزشکی زاهدان، دوره 13، شماره 6، صفحات: 19-13.
محمدی می­آبادی، ر.، حیدری نصرآبادی، م.، خدارحم، پ. و سیادت، ف. (1393). بررسی اثر عصاره هیدروالکلی دانه کرچک بر اسپرماتوژنز در موش. سال 13، دوره 2، شماره 50،  صفحات: 44-35.

Asgary, S., Parkhideh, S., Solhpour, A., Madani, H., Mahzouni, P. and Rahimi, P. (2008). Effect of ethanoic extract of Juglans regia L. on blood sugar in diabetes-induced Rats. Journal of Medicinal Food, 11(3): 533-538.

Borland, K. and Mita, M. (1985). The actions of insulin- like growth factor I and II on cultured sertoli cells. Endocrinology, 114: 240-246.

 Cunighum, J.G. (2002). Text book of Veterinary Physiology. 3rd ed., USA: Saunders, pp: 389-397.

 Cameron,D.F., Murray,F.T. and Drylie, D.D. (1985). Interstitial compartment pathology and spermatogenic disruption in testes from impotent diabetic men.  Anatomical Record, 231(1): 53-62.

DeFronzo, R.A. (1997). Pathogenesis of type 2 diabetes: metabolic and molecular implications for identifying diabetes genes. Diabetes Review, 5: 177-269.

Dhanasekaran, M. and Ren, J. (2005). The emerging role of coenzyme Q10 in aging neurodegeneration, cardiovascular disease, cancer and diabetic mellitus. Current Neurovascular Research, 2(5): 447- 459.

Iwasaki, M., Fuse, H. and Katayama, T. (1995). Histological and endocrinological investigations of cyclosporine effects on the rat testis. Andrologia, 27 (3): 185-189.

Littarru, G.P. (1994). Energy and Defense. Facts and perspectives on Coenzyme Q10 in biology and medicine. Casa Editrice Scientifica Internazionale, 1-91.

Nishimura, A., Fujimura, M., Hasegawa, F. and Nobuhito, S. (2010). Pharmacokinetic interaction between nifedipine and coenzyme Q10 in rat. Journal of Health Science, 56(3): 310-320.

Ricci, G., Catizone, A., Esposito, R., Pisanti, A., Vietri, M.T. and Galdieri, M. (2009). Diabetic rat testis: Morphological and functional alteration. Andrologia, 41: 361-368.

Swanson, J.E., Ben, R.N., Burton, G.W. and Parker, R.S. (1999). Urinary excretion of 2, 7, 8-trimethyl- beta-carboxyethyl)-6 hydroxychroman is a major route of elimination of gamma tocopherol in humans. Journal of Lipid Research, 40: 665-671.

Dey, L., Attele, A.S. and Yuan, C.S. (2002). Alternative therapies for type 2 diabetes. Alternative Medicine Review, 7(1): 45-58.

Foglia, V.G., Rosner, J.M., Ramos, M. and Lema, B.E. (1969). Sexual disturbances in the male diabetic rat. Hormone and Metabolic Research, 1: 72-77. Hassen, N.S., EI-roub, N.M. and

Omara, E.A. (2007). Evaluation of the influence of each of melatonin and chromium against diabetes-induced alteration in the testis of Albino rats using light and electron microscopies. Journal of Hospital Medicine, 27:143-162.

 Hughs, T., Gwynne, J. and Switzer, B. (1984). Effects of caloric restriction and weight loss on glycemic control, insulin release and resistance and atherosclerotic risk in obese patients with type ІІ diabetes mellitus. American Journal of Medicine, 77(1): 7-17.

 Quanhong, L., Caili, F., Yukui, R., Guanghui, H. and Tongyi, C. (2005). Effects of protein–bound polysaccharide isolated from pumpkin on insulin in diabetic rats. Plant Foods for Human Nutrition, 60: 13-16.

Lazos, E.S. (1986). Nutritional Fatty acids and oil characteristics of pumpkin and melon seeds. Journal of Food Science, 51(5): 1382-1383.

Noor, A., Gunasekaran, S., Soosai, M.A. and Vijayalakshmi, M.A. (2008). Antidiabetic activity of Aloe vera and histology of organs in streptozotocin induced diabetic rats. Current Science, 94: 8-25.

Ugbenyen, A.M. and Odetola, A.A. (2009). Hypoglycemic potential of young leave methanolic extract of Magnifera indica in alloxan induced diabetic rat. Pakistan Journal of Nutrition, 8(3): 239-241.