List of Articles Alloxan

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  1 - Effect of hydro-alcoholic extract of peel of Punica granatum L. on growth of Candida albicans in alloxan-induced diabetic rats
  M. Sadeghpour, M. Eidi, F. Noorbakhsh,
  Nowadays, especially opportunistic fungi such as Candida albicans are the most common riskfactors in patients who are immunocompromised. Fungal infections caused by Candida speciesand increasing strains resistant to azole drugs in Immunosuppression patients are importan More

  Nowadays, especially opportunistic fungi such as Candida albicans are the most common riskfactors in patients who are immunocompromised. Fungal infections caused by Candida speciesand increasing strains resistant to azole drugs in Immunosuppression patients are important toenjoy. The toxicity of drugs used, the resistance of the fungus and problems from druginteractions, necessitates the use of more effective and less toxic drugs cause the screw.Pomegranate consumption except for nutrition is impressive for medicinal and therapeuticeffects. The aim of this study was to investigate the effect of pomegranate peel extract at dosesconcentrations of 25, 50, 100 and 200 mg/kg on growth of C. albicans (ATCC 10231) inalloxan-induced diabetic rat. The animals were made diabetic by intraperitoneal injection ofalloxan (150 mg/kg). After diabetes induction, they were infected by Candida albicans, orally.Then, treatments of extract and Itraconazole were done orally. Sampling of fungus from month,culturing and colony counting were done daily for evaluating effects of extract and Itraconazoleon fungal growth. The results showed that oral treatment of hydro-alcoholic peel extractimproved Candida albicans infections in diabetic rats. Its potency is similar to Itraconazole, assynthetic drug. On the second day of treatment, significant effect of extract on growth C.albicans was shown (P<0.001). Comparison between effects of pomegranate extract andItraconazole on C. albicans was significant. The results suggest antifungal effect extract ofpomegranate compared with the antifungal medication. As a result, consumption ofpomegranate extract inhibited the growth of Candida albicans in the diabetic rats. Manuscript profile
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  2 - Comparison of the Effect of Hydroalcoholic Extract of Licorice (Glycyrrhiza glabra) and Its Active Ingredient Glycyrrhizic Acid on the Amount of Bax and Bcl-2 Proteins in the Liver Cells of Type 1 Diabetic Male Rats
  Sedigheh Jani Vida Hojati Gholamhassan Vaezi Raheleh Rahbarian
  Introduction and Objective: The function of the liver as an insulin-dependent organ is strongly affected by diabetes. Diabetes can lead to liver damage through oxidative stress. The present study was performed to evaluate the possible effects of hydroalcoholic extract o More

  Introduction and Objective: The function of the liver as an insulin-dependent organ is strongly affected by diabetes. Diabetes can lead to liver damage through oxidative stress. The present study was performed to evaluate the possible effects of hydroalcoholic extract of licorice (Glycyrrhiza glabra) and glyceric acid on Bax and Bcl2 apoptotic factors in male type 1 diabetic rats. Material and Methods: In this experimental study, 72 male Wistar rats were injected into diabetic control, diabetic control and diabetic groups treated with hydroalcoholic extract of licorice and glycyrrhizinic acid at concentrations of 50 and 100 mg / kg by injection. The time intervals of 15 and 30 days were divided. Diabetic control and treatment groups became diabetic with a single intraperitoneal injection of 120 mg/kg alloxane monohydrate. At the end of the treatment period, Bax and Bcl2 apoptotic factors were measured. The results were analyzed by SPSS 21 software and one-way analysis of variance and Tukey post hoc test. Results: Bax apoptotic factor was significantly decreased in the treated groups compared to the diabetic control groups and Bcl2 anti-apoptotic factor was significantly increased (p <0.05) which indicates improvement in the structure of damaged liver tissue in the groups. It is a treatment. Conclusion: Hydroalcoholic extract of licorice and glycyrrhizic acid is in expressed doses of anti-apoptotic and has a protective effect against liver damage induced by diabetes. Manuscript profile
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  3 - Tandem Synthesis and Optical Rotatory Dispersion Studies of a Novel Spiro Lactone (methyl 3-(benzo[d]thiazol-2-ylamino)-7,9-dimethyl-2,6,8,10-tetraoxo-1-oxa-7,9-diazaspiro[4.5]dec-3-ene-4-carboxylate)
  Karim Ghafouri Samira Arab-salmanabadi Sajjad Sedaghat
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  4 - Evaluation of cardiac injury biomarkers of serum in alloxan induced diabetic rats
  Yaghoub Haji-Sadeghi majid fartashvand
  Diabetes mellitus is a metabolic disorder and its incidence is considered to be high all over the world. It causes increase in blood glucose levels and subsequent damage to various tissues and blood vessels. The aim of the present study was to evaluate cardiac injury bi More

  Diabetes mellitus is a metabolic disorder and its incidence is considered to be high all over the world. It causes increase in blood glucose levels and subsequent damage to various tissues and blood vessels. The aim of the present study was to evaluate cardiac injury biomarkers of serum in rats with experimental diabetes. Thirty male Wistar rats were randomly assigned into 2 groups including healthy control and diabetic rats. Diabetes was induced with a single injection of alloxan (120 mg/kg IP). Four weeks after induction of diabetes, levels of cTnI (cardiac troponin I), AST (Aspartate aminotransferase), LDH (lactate dehydrogenase) and CK-MB (keratin kinase) were measured in serum. At the end of the experiment, mean blood sugar levels were 263.1 ± 36 and 96.7 ± 12 mg/dl in diabetic and control groups respectively that reveals a statistically significant difference between the two groups (p=0.000). Serum cTnI level in diabetic rats was significantly higher than healthy control group (p=0.002). Other cardiac biomarkers of serum in experimentally induced diabetic rats were significantly higher than control group. There were significant correlation between blood glucose levels and serum cTnI (r=0.685; p=0.010), and with serum activities of enzymes, including AST (r=0.862; p=0.000), LDH (r=0.760; p=0.008) and CK-MB (r=0.590; p=0.014). In conclusion, elevated level of serum cTnI and enzyme activities in diabetic rats in comparison to the control group suggests some degree of cardiac muscle damages. Manuscript profile
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  5 - protective effect of Naringenin (Citrus flavonone) on incipient diabetic nephropathy in the rats with alloxan-induced diabetes
  Yousef Doustar رامین Kaffash Elahi داریوش Mohajeri
  Diabetes mellitus is a metabolic abnormality that has a relatively high prevalence all over the world. Kidney failure is one of the main complications of diabetes. Many therapeutic methods have been introduced from all over the world to treat diabetes. The aim of the pr More

  Diabetes mellitus is a metabolic abnormality that has a relatively high prevalence all over the world. Kidney failure is one of the main complications of diabetes. Many therapeutic methods have been introduced from all over the world to treat diabetes. The aim of the present study was to assess the protective effect of Naringenin on early kidney injuries in alloxan-induced diabetic rats. Forty male Wistar rats were randomly allocated into 4 equal groups, including: healthy control, normal healthy receiving Naringenin, diabetic and diabetic receiving Naringenin. Diabetes was also induced by intraperitoneal injection of a single dose of naloxone (120 mg/kg). Naringenin treatment groups received the drug (50 mg/kg) daily for 3 weeks through the gavage. Finally, serum levels of kidney function markers including urea, uric acid and creatinine as well as amount of lipid peroxidation product (MDA) and activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) and glutathione reductase (GR) were assessed in kidney hemogenates. Moreover, histopathological observation was assayed at the degree of renal injury. Significant differences among the groups were determined by one-way analysis of variance followed by Tukey post-test. Statistical significance was considered at p<0.05. In alloxanized diabetic rats, Naringenin significantly decreased the levels of serum urea, uric acid and creatinine (p<0.05), and significantly decreased the lipid peroxidation and elevated the levels of antioxidant enzymes in these rats (p<0.05). Histopathological changes were in agreement with biochemical findings. The results of the present study showed that naringenin with antioxidant properties can prevent early diabetic kidney damage. Manuscript profile
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  6 - Study on protective effect of Naringenin (Citrus flavonone) on incipient diabetic hepatopathy in alloxan-induced diabetic rats
  daryoush mohajeri ghafour mousavi ramin kaffashielahi mehrdad neshatgharamaleki
  Abstract    Diabetes mellitus is a metabolic disorder and its incidence is considered to be high all over the world. Hepatic insufficiency is one of the most important consequences in this disease. A multitude of drugs has been described for the treatment of More

  Abstract    Diabetes mellitus is a metabolic disorder and its incidence is considered to be high all over the world. Hepatic insufficiency is one of the most important consequences in this disease. A multitude of drugs has been described for the treatment of diabetes throughout the world. The aim of the present study was to assess the protective effect of Naringenin on early liver injury in alloxan-induced diabetic rats. Forty male Wistar rats were randomly assigned into 4 different groups of 10 rats each, including healthy control rats, normal healthy rats receiving Naringenin (50 mg/kg), diabetic rats and diabetic rats receiving Naringenin (50 mg/kg). Diabetes was induced with a single injection of alloxan (120 mg/kg i.p.). Naringenin groups received the drug daily for 3 weeks through gavage. At the end of the experiment, levels of liver function marker enzymes AST (Aspartate aminotransferase), ALT (Alanine aminotransferase) and ALP (Alkaline Phosphatase), TB (Total Bilirubin), Alb (Albumin) and TP (Total Proteins) were assessed in serum. Product of lipid peroxidation (Malondialdehyde; MDA), activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) and glutathione reductase (GR) were also assayed in liver homogenate to evaluate antioxidant activity. Moreover, histopathological observations were made to assess the degree of hepatic injury. In alloxanized diabetic rats, Naringenin significantly decreased the levels of serum biomarkers of hepatic injury and TB, and elevated the levels of Alb and TP. Furthermore, Naringenin significantly decreased the lipid peroxidation and elevated the levels of antioxidant enzymes in these rats. Histopathological changes were in agreement with biochemical findings. The findings of this study indicated that Naringenin due to its antioxidant activities protects rats liver from early diabetic hepatopathy. Manuscript profile
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  7 - Histopathological study of the protective effects of ethanolic extract of Crocus sativus L. (Saffron) stigma on hepatic and renal tissue changes in alloxanized diabetic rats
  داریوش Mohajeri مهران Mesgari Gh Mousavi یوسف Doustar بهرام Amouoghli Tabrizi
  Diabetes mellitus is a metabolic disorder affecting mankind and its incidence is considered to be high all over the world. Hepatic and renal insufficiencies are identified as the most important causes of death in this disease. A multitude of herbs has been described for More

  Diabetes mellitus is a metabolic disorder affecting mankind and its incidence is considered to be high all over the world. Hepatic and renal insufficiencies are identified as the most important causes of death in this disease. A multitude of herbs has been described for the treatment of diabetics throughout the world. The aim of the present study was to assess the protective effects of ethanolic saffron stigma extract (Crocus sativus L.) on early hepatic and renal injuries in alloxan-induced diabetic rats.  For this purpose, 72 male wistar rats were randomly assigned to six different groups of 12 rats each, including healthy control rats, healthy control rats receiving ethanolic saffron extract, mild diabetic (MD) rats, mild diabetic rats receiving ethanolic saffron extract, severely diabetic (SD) rats and severely diabetic rats receiving ethanolic saffron extract. The most effective dose of the extract in reducing blood glucose (40 mg/kg), dissolved in normal saline solution (10 ml/kg) was administered intraperitoneally to treatment groups receiving the extract for 30 consecutive days. Control groups received normal saline solution in the same manner. Four hours after the last extract injections, all rats were euthanized simultaneously by cervical dislocation. Tissue specimens were fixed in 10% buffered formalin and 5 micron thick sections were prepared using routine hitopathological techniques. Histopathology of the liver and kidney in diabetic rats showed a spectrum of changes including periportal degeneration, membranoproliferative glomerulitis, enlargement of lining cells of tubules, lymphocytic infiltration, hyperemia and hemorrhage. These injuries were more prominent in SD rats. The histopathological appearance of liver and kidneys in ethanolic extract treated diabetic rats was near normal. The findings of the present study indicate that ethanolic extract of Crocus sativus L. stigma has protective effects on early diabetic nephropathy and hepatopathy in experimentally induced diabetes. Therefore, ethanolic extract of Crocus sativus L. stigma is recommended for prevention of early renal and hepatic injuries in diabetes mellitus, after randomized clinical trials. Manuscript profile
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  8 - The effect of coenzyme Q10 on the prevention of testicular tissue damages in Alloxan-induced diabetic rats
  seyyed sajjad hejazi ramin jahangirfard
  Coenzyme Q10 is considered as a vitamin or a vitamin-like substance which is found in food resources naturally like other vitamins. In recent years, several drugs have been used effectively for treatment of diabetes, but the treatment or protective effect of few of them More

  Coenzyme Q10 is considered as a vitamin or a vitamin-like substance which is found in food resources naturally like other vitamins. In recent years, several drugs have been used effectively for treatment of diabetes, but the treatment or protective effect of few of them have been studied microscopically. In the present study, the complementary effect of Q10 on testicular structure in alloxan-induced diabetic rats was studied. Forty male Wistar rats were randomly divided into four groups of 10 rats. The first group was the control group; the second group was the control group treated with 75 mg/kg Q10 for one month, orally (gavage); the third one was the alloxan-induced diabetic treatment group in which a single dosage of 120 mg/kg alloxan was administered intra peritoneally, and the fourth group was the diabetic treatment group treated with Q10. The rats’ weight, testis weight, seminiferous tubule diameter, testicular capsule thickness and seminiferous tubular epithelium thickness were measured. Statistical analysis was performed using ANOVA and Duncan,s multiple range test. Q10 demonstrated a modifying effect on the diabetic rats’ testicular damage such that damages in tissue structure and the sex cells, as well as shrinkage of the seminiferous tubules were observed very slightly. Furthermore, Q10 could inhibit the infiltration of the vessels into and under the capsule space which was significant in diabetic group. The use of Q10 as an antioxidant significantly protected the spermatogenic cells, testicular capsule, testis weight, and seminiferous tubule diameter from the damage of diabetes in rats. Manuscript profile
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  9 - The effects of date consumption on serumic levels of glucose, lipids and lipoproteins in diabetic rats
  بهرام Amouoghli Tabrizi علی Hassanpour وحید Kohi احمد Ostovar آرش Alizade
   Diabetes mellitus is an important problem amongst human and animals. In veterinary medicine too, diabetes mellitus occurs in many animals particularly household pets. This study was conducted in order to evaluate the effect of date consumption on serumic levels of More

   Diabetes mellitus is an important problem amongst human and animals. In veterinary medicine too, diabetes mellitus occurs in many animals particularly household pets. This study was conducted in order to evaluate the effect of date consumption on serumic levels of glucose, triglyceride, cholesterol, LDL, VLDL and HDL in rats suffering from experimental diabetes mellitus. Thirty male wistar rats with the age of 8 weeks and mean body weight of 200±20 gr were selected and divided into 5 groups so that there were 6 rats per group. The groups consisted of day one healthy control, final day healthy control, healthy treatment consuming date, diabetic treatment consuming date and diabetic control group. Diabetic treatment and control groups received 100 mg/kg of alloxan subcutaneously to create experimental diabetes. The two healthy control groups also received equal amounts of normal saline solution subcutaneously. The injections were repeated a week later in all groups. After observing the diabetes symptoms including polydipsia, polyuria, glucosuria and hyperglycemia in groups that were given alloxan, feeding of healthy and diabetic treatment groups with equal ratio of date and pellet was initiated. Final day healthy control group and diabetic control group were fed only with pellet. The groups were fed for 10 days following the start of diabetes symptoms. Blood samples were collected from all groups on day 10. Evaluation of the serumic levels of glucose, cholesterol and LDL revealed statistically significant increase in diabetic control group in comparison with the healthy control groups and also in healthy and  diabetic treatment groups consuming date as compared with diabetic control and healthy groups (p<0.05). Evaluation of the serumic levels of triglyceride in diabetic and healthy treatment groups consuming date did not reveal statistically significant difference with the healthy control groups but revealed a significant decrease in comparison with the diabetic control group. Serumic levels of VLDL in diabetic and healthy treatment groups consuming date indicated significant decrease compared with the diabetic control group (p<0.05) but the changes were not significant in comparison with the healthy control groups. There were no statistically significant differences in serumic levels of HDL amoung the groups. It can be concluded from these results that consumption of date as a natural fruit can be effective in preventing symptoms of diabetes mellitus.   Manuscript profile
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  10 - اثر روغن گلرنگ بر قند خون و پروفایل لیپیدی در موشهای صحرایی دیابتی شده با آلوکسان و مکانیزم آن
  پریوش رحیمی محبوبه سترکی منیر ویودی
  مقدمه و هدف: دیابت ملیتوس اغلب به عنوان یک سندروم ناهنجاری متابولیسمی شناخته می شود، که معمولا بدلیل ترکیبی از عوامل ارثی ومحیطی رخ می دهد که منجر به افزایش غیر عادی میزان قند خون(هیپرگلیسمی) میشود.امروزه، استفاده از درمان‌های غیردارویی (گیاهان دارویی) رویکرد جدیدی در ک More

  مقدمه و هدف: دیابت ملیتوس اغلب به عنوان یک سندروم ناهنجاری متابولیسمی شناخته می شود، که معمولا بدلیل ترکیبی از عوامل ارثی ومحیطی رخ می دهد که منجر به افزایش غیر عادی میزان قند خون(هیپرگلیسمی) میشود.امروزه، استفاده از درمان‌های غیردارویی (گیاهان دارویی) رویکرد جدیدی در کنترل بیماری‌ دیابت است.  هدف از مطالعه حاضر بررسی اثر روغن گلرنگ بر قند خون و پروفایل لیپیدی در موش‌های صحرایی دیابتی بود.  روش تحقیق: در این تحقیق 18 سر موش صحرایی نر در سه گروه شش تایی تقسیم شدند: کنترل ، دیابتی تیمار شده با گلی بن کلامید و دیابتی تیمار شده با روغن ‌گلرنگ به میزان 200 میلی‌گرم بر کیلوگرم وزن بدن. برای دیابتی کردن، از آلوکسان به میزان 120 میلی گرم بر کیلوگرم وزن بدن به صورت تزریق درون صفاقی استفاده شد.  روغن ‌گلرنگ به میزان 200 میلی‌گرم بر کیلوگرم وزن بدن روزانه در یک دوره زمانی 30 روزه استفاده شد.  نتایج و بحث:نتایج بدست آمده از این تحقیق نشان داد که روغن ‌گلرنگ به‌طور معنی‌داری دارای اثر هیپوگلیسمیک و هیپولیپیدمیک در موش های صحرایی دیابتی بوده است. در واقع، ترکیبات فنولی و اسیدهای چرب روغن گلرنگ نقش مهمی در تنظیم ترشح انسولین  و هموستازی گلوکز ایفا می کنند. Manuscript profile
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  11 - اثرات ضد دیابتی و سم شناسی عصاره آبی برگ Ocimum gratissimum در موشهای دیابتی ناشی از آلوکسان
  عبدالکریم محمود بوخاری محمود آدنیک اولادیجی
  10.30495/medherb.2021.681960
  Background & Aim:Ocimum gratissimumis an aromatic plants used among traditional medicine practitioners in the treatment of warts, diarrhoea, headache, diabetes etc. This study aimed at evaluating the anti-diabetic and toxicity profile of aqueous leaf extract of the More

  Background & Aim:Ocimum gratissimumis an aromatic plants used among traditional medicine practitioners in the treatment of warts, diarrhoea, headache, diabetes etc. This study aimed at evaluating the anti-diabetic and toxicity profile of aqueous leaf extract of the plant in alloxan-induced diabetic rats. Experimental: Thirty albino rats (111.33 ±1.50g) were grouped into six (A-F) groups of animals. Group A received 0.5 ml distilled water (p.o) for eight days. Diabetes was induced in group B-F animals using 160 mg/kg alloxan (i.p < /em>) and thereafter administered 2.5 metformin, 125, 250 and 500 mg/kg aqueous leaves extract of Ocimum gratissimum, respectively (p.o) for 8 consecutive days. Blood sugar level was taken 1 h after drug administration every other day. Body weights of animals were taken before induction, after induction, and on the 8th day. Blood samples and organs (liver, kidney, and pancreas) were collected for biochemical assays and histopathological examinations. Results: Alloxan significantly (p < 0.05) increased the glucose, albumin, urea, creatinine, bilirubin, Alkaline phosphatase (ALP), Aspartate transaminase (AST) and Alanine transaminase (ALT) levels of rats compared with the distilled water group. The aqueous leaves extract of Ocimum gratissimum significantly (p < 0.05) reduced the glucose, albumin, urea, creatinine, bilirubin, ALP, AST and ALT levels compared with the diabetic untreated rats. There were no significant histological changes in the liver, pancreas and kidneys of diabetic treated rats compared with diabetic untreated rats which exhibited moderately distorted organ degeneration. Recommended applications/industries: Aqueous leaves extract of Ocimum gratissimum possesses anti-hyperglycemic effects and is relatively safe for use in the treatment of diabetics. Manuscript profile
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  12 - اثرات آنتی بیوتیکی و آنتی اکسیدانی عصاره متانولی برگ گیاه Momordica charantia بر فشار خون ناشی از آلوکسان در موش صحرایی
  ساندی افوگ الوفکه فلایی بلسین اگونپلو آدمولا اویابمی تمیدایو اوموبوال مومو یکوبو اولووافمی اوگونتیبجو آدلو آدداپو
  Background & Aim:The plant Momordica charantia is highly valuable having a wide range of medicinal uses with tropical and subtropical regions distribution. This study was conducted to appraise the medicative properties of methanol leaf extract of Momo More

  Background & Aim:The plant Momordica charantia is highly valuable having a wide range of medicinal uses with tropical and subtropical regions distribution. This study was conducted to appraise the medicative properties of methanol leaf extract of Momordica charantia (MEMC) on alloxan-induced diabetic rats. Experimental: The antidiabetic influence of methanol leaf extract of Momordica charantia (MEMC) was investigated in alloxan-induced diabetes in rats. 50 rats allocated into five groups (A-E) (n =10) were utilized in this study: group A was normal control, groups B to E were induced with alloxan with diabetes established, while group B was not treated, group C received glibenclamide treatment while groups D and E were administered extracts at 200 and 400 mg/kg doses, respectively, in a study that continued for 28 days. Changes in blood glucose levels, body weight, haematology, serum chemistry, antioxidant system and histopathology changes were evaluated. Results: MEMC elicited significant drop in blood glucose level from diabetic to near normal level, restoring the body weight, haematological and serum biochemical parameters to the basal non diabetic level; likewise the MEMC-treated group elicited a decreased level of oxidant markers such as malondialdehyde (MDA) and hydrogen peroxide (H2O2) but increased concentration of protein thiols (PT), non-protein thiols (NPT), glutathione (GSH), glutathione peroxidase (GPx), glutathione-S-transferase (GST) and superoxide dismutase (SOD) indicating its anti-oxidant potential. The pancreas section also revealed repair of distorted pancreatic architecture in MEMC-treated group compared to diabetic group. Recommended applications/industries: The plant exhibited anti-hyperglycaemic, anti-diabetic and anti-oxidant abilities hence could be explored for the treatment of diabetes mellitus. Manuscript profile
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  13 - The Effect of Q10 on Reducing of Induced Diabetic Kidney Tissue Injury and Improvement of the Serum Levels of Renal Functional Factors in Rat
  سید سجاد حجازی
  The present study was conducted to evaluate the protective effects of Q10 on the damage of kidney tissue as well as serum factor levels due to diabetes induction in Alloxan-induced diabetic rats. The experimental model of diabetes type A in rats was induced by intraperi More

  The present study was conducted to evaluate the protective effects of Q10 on the damage of kidney tissue as well as serum factor levels due to diabetes induction in Alloxan-induced diabetic rats. The experimental model of diabetes type A in rats was induced by intraperitoneally injection of 120 mg Alloxan monohydrate per kg/bw. Physiology serum was used as Alloxan solvent. Control group rats received buffer citrate 0.05 M (pH= 4.5), intraperitoneally. The Q10- treatment group received 75 mg/kg Q10 via gavage for one month. In Alloxan-Q10 treatment group, the rats at first were diabetic and then they received 75 mg/kg Q10 via gavage for one month. Severe tissue damage was observed in the obtained tissue samples from diabetic rats. The tissue damages were in the forms of acute tubular necrosis, interstitial tubular nephrosis, vacuolar alterations, and atherosclerosis. The obtained results from urea, uric acid, and creatinine levels in understudied rats demonstrated a significant difference between diabetic-treatment group and diabetic-Q10 group (p Manuscript profile
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  14 - Clinical and microscopic study of the effect of different concentrations of cardamom extract (Elettaria cardamomum) on the healing of skin wounds in diabetic rabbits with alloxan
  Fatemeh Tavakoli Rad loghman Akradi Mahdieh Raeeszadeh
  10.30495/vmj.2021.687383
  In this study, 30 male rabbits weighing 900 - 1000 g were randomly divided into 6 groups (positive control, negative control and test). After general anesthesia using a combination injection of ketamine 50 mg / kg and xylazine 5 mg / kg intraperitoneally to create ulcer More

  In this study, 30 male rabbits weighing 900 - 1000 g were randomly divided into 6 groups (positive control, negative control and test). After general anesthesia using a combination injection of ketamine 50 mg / kg and xylazine 5 mg / kg intraperitoneally to create ulcers in the rabbit skin a 10 mm circular punch was made. The wounds was measured with the aid of millimeter papers on the first, third, sixth, ninth, twelfth, fifteenth, eighteenth, and twenty-first days after surgery. This measurement is made at a specific time of day by a specific person. Complete recovery time was measured in negative, positive control and test samples. Then, on day 21, the wound was sampled. The specimens were subjected to histopathological studies after fixation of dewatering and staining.According to the results, there was no significant difference between control group and T1 group (P <0.05), but there was a significant difference between control group and T2, T3, T4, T5 group (P <0.05). The highest difference was between T5 and control group. It can be said that methanolic extract of cardamom at a dose of 400 mg / kg topical and feeding on cardamom powder pellets was more effective than all groups. Also, migration of epithelial tissue and reduction of wound openings in group T5 on days 15 to 21 were higher than other groups. Also, cardamom powder as the last plate of food significantly decreased glucose in diabetic groups (P <0.05). Cardamom lowers blood sugar by antioxidants such as flanoids. According to the results of this study, cardamom extract has a high ability to heal full-thickness wounds compared to chemical drugs. Manuscript profile