Investigation of antibiotic residues in edible tissues of slaughtered broilers in Maragheh abattoir using FPT method (short comunication)
Subject Areas : Food Science and TechnologyMasumeh Abasi 1 , Saman Mahdavi 2
1 - Department of Microbiology, Malekan Branch, Islamic Azad University, Malekan, Iran
2 - Department of Microbiology, Maragheh Branch, Islamic Azad University, Maragheh, Iran
Keywords: broilers, Antibiotic residues, Four plate test method,
Abstract :
Antibiotic residues in food stuff and their transmission to the consumers have some consequences such as bacterial resistance, allergic reactions, intoxication, carcinogenic effects and disturbing of intestine natural flora. Among microbiologic methods, four plate test (FPT) is used to detect antibiotic residues in food stuff, which performs in four culture media with different pH values and test bacteria. The aim of this study was investigation of antibiotic residues in edible tissues of slaughtered broilers in Maragheh abattoir using FPT method. For this reason, 40 slaughtered broilers carcasses in Maragheh abattoir (from 10 different poultry farms) were sampled. The sampling was conducted randomly from breast and leg muscles, gizzard as well as liver of each carcass. According to results of current study, 60% of liver samples, 55% of leg samples, 52.5% of breast samples and 52.5% of gizzard samples contained antibiotic residues. Moreover, the amount of antibiotic residues among different samples did not show statistical significance (p>0.05). The highest occurrence of antibiotic residue was found in two flocks (100%) and the lowest occurrence was recorded for another two flocks (0%). According to the health hazard of antibiotic residues in foods, continuous monitoring is recommended for edible tissues of broilers.
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_||_● Anonymous (1992). Practical Microbiology Guideline. Merk’s Trading Publishing .pp. 1-15.
● Berends, B.R., Van den Bogaard, A.E., van Knapen, F. and Snijders, J.M. (2001). Human health hazards associated with the administration of antimicrobials to slaughter animals, Veterinary Quarterly, 23(1): 2-10.
● Botsoglou, N.A. and Fletouris, D.J. (2001). Drug Residue in Foods. New York. Marcel Dekker. pp. 456-572.
● Dewdney, J.M., Mesa, L., Raynaud, J.P., Blanc, F., Scheid, J.P., Jackson, T., Lens, S. and Vershvers, C. (1991). Risk assessment of antibiotic residues of betalactams and macrolids in food products with regard to their immunoallergic potential. Journal of Food Chemistry and Toxicology, 26: 477-483.
● Elnasri Hind, A., Salman Adil, M. and El Rade, S.A. (2014). Screening of antibiotic residues in poultry liver, kidney and muscle in Khartoum state,Sudan. Journal of Applied and Industrial Sciences, 2(3):116-122.
● Institute of Standards and Industrial Research of Iran (1379). Control instructions veterinary drugs, Standard, No. 5592. [in Persian]
● Karim, G., Kiaei, S.M.M., Rokni, N. and Razavi Rouhani, S.M. (2011). Antibiotic residue contamination in milk during last forty years in Iran, Journal Food Hygiene, 1(1): 23-30. [in Persian]
● Khan Nazer, A.H., Hoseinzadeh, S. and Parvandeh, H. (1999). Determination of antibiotic residues in the poultry carcasses using four plates tested in slaughterhouses around Shiraz, Journal of Veterinary Research of Tehran University, 54(3): 83-79. [in Persian]
● Khan Nazer, A.H., Shekarfrosh, S.Sh. and Ghanei, H. (1995). Means of F.P.T (four –plate – test) method for determination of antibiotic residues in carcasses of sheep, Pajouhesh-va-Sazandegi, No. 26: 183-180. [in Persian]
● Khan Nazer, A.H. and Kahba, H. (1999). Study of antibiotics and sulfonamide residues in poultry with Means of F.P.T (four –plate – test) method and thermal effect on them, Pajouhesh-va-Sazandegi, No. 43: 65-62. [in Persian]
● Pavlov, Al., Lashev, L., Vachin, I. and Rusev, V. (2008). Residues of antimicrobial drugs in chicken meat and offals. Trakia Journal of Sciences, 6: 23-25.
● Rokni, N., Kamkar, A., Salehzadeh, F. and Madani, R. (2007). Study on the Enrofloxacin Residues in Chicken Tissues by HPLC, Iranian Journal of Nutrition Sciences & Food Technology, 4(2): 17-11. [in Persian]
● Tajick, M.A. and Shohreh, B. (2006). Detection of antibiotics residue in chicken meat using TLC. International Journal of Poultry Science. 5(7): 611-612.
● Torbati, M.A., Shamshiri, M. and Javadi, A. (2011). Detection of Antibiotic Residues in Edible Tissue of Slaughtered Cows in Tabriz Abattoir with FPT Method, Journal of Food Hygiene, 1(2): 29-37. [in Persian]
● Vahedi, N., Motaghedi, A. and Golchin, M. (2011). Determination of antibiotic residues in industrial poultry carcass by means of F.P.T (four –plate – test) method in Mazandaran province, Iranian Journal of Nutrition Sciences & Food Technology, 8(1): 72-65. [in Persian]
