Determination of Zn, Pb, Cd and Cu contents in raw milk from Khorram-Abad dairies
Subject Areas : Food Science and TechnologyS. Sobhanardakani 1 , محسن Tizhosh 2
1 - Associate Professor of Department of Environment, Hamedan Branch, Islamic Azad University, Hamedan, Iran
2 - M.Sc. Graduated in Environmental Sciences, Hamedan Branch, Islamic Azad University, Hamedan, Iran
Keywords: Heavy metals, Lorestan province, Food Safety, raw milk,
Abstract :
Heavy metals are the most important chemical pollutants of foods. Milk and dairy products due to the nutritional functions are largely consumed by infants and children around the world. The purpose of this study was to analyze Zn, Pb, Cd and Cu contents in raw cow milks produced in dairies of Lorestan industrial livestock zone during 2014. A total of 24 milk samples was collected from eight dairies. The samples were subjected to acid digestion according to standard method. Afterwards, the metal contents were determined using ICP-OES. All statistical analyses were performed using the SPSS statistical package. The results showed that mean concentrations of Zn, Pb, Cd and Cu in milk samples were 3072±674, 2720±2790, 104±18.5 and 142±149 µg/kg, respectively. Also comparison of the mean concentrations of the metals with the WHO maximum permissible limits in milk revealed that the mean concentrations of all metals were significantly higher than permissible limits. Because of the mean concentrations of Zn, Pb, Cd and Cu in milk samples were higher than Mean Residual Limits, therefore to achieve the consumers’ safety, monitoring of feed intake, water supplies, soil properties around livestock and forage crops and control of pollutants emission from industries near the livestock are recommended.
• بنیادیان، مجتبی؛ مشتاقی، حمدالله و سلطانی، زینب (1385). بررسی میزان سرب و کادمیوم در شیرهای خام و پاستوریزه تولید شده در منطقه شهرکرد.مجله دامپزشکی ایران، دوره 2، شماره 2، صفحات: 81-74.
• دبیری، مینو (1389). آلودگی محیطزیست (هوا، آب، خاک، صوت)، انتشارات اتحاد، تهران، صفحه 400.
• رحیمی، ابراهیم و نیک‌نشان، سهیل (1386). تعیین غلظت سرب و کادمیوم در شیر خام در منطقه زرین شهر. مجله دامپزشکی دانشگاه آزاد اسلامی واحد گرمسار، دوره 3، شماره 1، صفحات: 34-29.
• رکنی، نوردهر (1389). اصول بهداشت مواد غذایی، انتشارات دانشگاه تهران، تهران، صفحه 161.
• قزلباش، مهسا؛ دربانی، سیدمحمدرضا؛ روستا، سرور؛ اسلامی مجد، عبدا...؛ سلطان الکتابی، محمود و موسوی‌آذر، علی (1394). کاربرد بیناب نمایی فروشکست القایی لیزری در شناسایی و تعیین غلظت عناصر موجود در شیر. مجله علوم تغذیه و صنایع غذایی ایران، سال 10، شماره 1، صفحات: 96-89.
• وحیدی‌نیا، علی اصغر؛ صالحی، ایرج؛ بیگی‌نژاد، هادی.، پورتقی، جلال؛ نظری، زهرا و مرادی، محمدرضا (1392). مطالعه میزان سرب و کادمیوم و عوامل مرتبط با آن در شیرهای خام تولیدی در مناطق مختلف استان همدان. بهداشت مواد غذایی، دوره 3، شماره 3، صفحه: 47-39.
● Akalın, A.S., Unal, G., Dinkci, N., and Hayaloglu, A.A. (2012). Microstructural, textural, and sensory characteristics of probiotic yogurts fortified with sodium calcium caseinate or whey protein concentrate. Journal of Dairy Science, 95: 3617–3628.
● Akin, M.B., Akın, M.S., and Kırmacı, Z. (2007). Effects of inulin and sugar levels on the viability of yogurt and probiotic bacteria and the physical and sensory characteristics in probiotic ice-cream. Food Chemistry, 104: 93–99.
● Dols, J.A.M., Boon, M.E., Monachese, M., Changalucha, J., Butamanya, N., Varriano, S., Vihant, O., Hullegie, Y., van Tienen, A., Hummelen, R., and Reid, G. (2011). The impact of probiotic yogurt on HIV positive women in Tanzania. International Dairy Journal, 21: 575–577.
● Donkar, O.N., Nilmini, S.L.I., Stolic, P., Vasiljevic, T., and Shah, N.P. (2007). Survival and activity of selected probiotic organisms in set-type yogurt during cold storage. International Dairy Journal, 16: 1181–1189.
● Erdem, O., Gultekin-ozguven, M., Berktas, I., Ersan, s., Tuna, H.E., Karadag, A., and Ozcelik, B. (2013). Development of a Novel Synbiotic Dark Chocolate Enriched with Bacillus indicus HU36, Maltodextrin and Lemon Fiber: Optimization by Response Surface Methodology. Food Science and Technology. 56 (1): 187–193.
● Farnworth, E.R., Mainville, I., Desjardins, M.P., Gardner, N., Fliss, I., and Champagne, C. (2007). Growth of probiotic bacteria and bifidobacteria in a soy yogurt formulation. International Journal of Food Microbiology, 116: 174–181.
● Hoppert, K., Zahn, S., Jänecke, L., Mai, R., Hoffmann, S., and Rohm, H. (2013). Consumer acceptance of regular and reduced-sugar yogurt enriched with different types of dietary fiber. International Dairy Journal, 28: 1–7.
● Irkin, R., and Guldas, M. (2011). Evaluation of cacao-pudding as a probiotic food carrier and sensory acceptability properties. Acta agriculturae Slovenica, 97: 223-232.
● Institute of Standards and Industrial Research of Iran (2008). Probiotic yogurt - Characteristics and test methods. Standard No. 11325. [in Persian]
● Institute of Standards and Industrial Research of Iran (2008).Yoghurt-Characteristics and test methods. Standard No. 695. [in Persian]
● Institute of Standards and Industrial Research of Iran (2007). Milk and dairy products - Enumeration of colony forming units mold or yeast - colony count at 25 ° C in the plate. Standard No. 10154. [in Persian]
● Institute of Standards and Industrial Research of Iran (2007). Microbiology of food and animal feeding stuffs - Comprehensive method for counting coliforms - colony count method - national standard of 9263. [in Persian]
● Institute of Standards and Industrial Research of Iran (2006). Determination of acidity titratable and potentiometric ph in milk and milk products, 1st Edition, Standard No. 2852. [in Persian]
● Institute of Standards and Industrial Research of Iran (2005). Detection and enumeration of presumptive Escherichia coli in foods - most probable number technique, 1st Revision, 3rd Edition, Standard No. 2946. [in Persian]
● Institute of Standards and Industrial Research of Iran (2005). Microbiology of food and animal feeding stuffs - enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) - Test Method, Part I, using medium-range Culture medium Bard - Parker agar. Standard No. 1-6806. [in Persian]
● Institute of Standards and Industrial Research of Iran (2004). Milk and dairy products - raw milk specifications and test methods. Standard No. 164. [in Persian]
● Institute of Standards and Industrial Research of Iran (2002). Cheese and processed cheese - Determination of total dry matter (Reference method) - Test Method (revised). Standard No. 1753. [in Persian]
● Institute of Standards and Industrial Research of Iran (2002). Milk and dairy products - total count of colonies of microorganisms at 30 degrees Celsius. Standard No. 5484. [in Persian]
● Institute of Standards and Industrial Research of Iran (1991). Measurement Milk fat. Standard No. 366. [in Persian]
● Jayeola, C. O., Yahaya, L. E., and Igbinadolor, R. O. (2010). Cocoa powder supplementation in yoghurt production. Journal of Food Technology, 3: 82-85.
● Lollo P.C.B., de Moura, C.S., Morato, P.N., Cruz, A.G., and Castro, W.F. (2013). Corrigendum to “Probiotic yogurt offers higher immune-protection than probiotic whey beverage. Food Research International, 54: 923.
● Madhu, A.N., Amrutha, N., and Prapulla, S.G. (2012). Characterization and antioxidant property of probiotic and synbiotic yogurts. Probiotics and Antimicrobial Proteins, 4: 90-97.
● Marafon A.P., Sumi, A., Granato, D., Alcântara, M.R., Tamime, A.Y., and Nogueira de Oliveira, M. (2011). Effects of partially replacing skimmed milk powder with dairy ingredients on rheology, sensory profiling, and microstructure of probiotic stirred-type yogurt during cold storage. Journal of Dairy Science, 94: 5330–5340.
● Matto, J., Malinen, E., Suihko, M. L., Alander, M., Palva, A. and Saarela, M. (2004). Genetic heterogeneity and technological properties of intestinal bifidobacteria. Journal of Applied Microbiology. 97: 459–470.
● Possemiers, S., Marzorati, M., Verstraete, W., and Van de Wiele, T. (2010). Bacteria and chocolate, A successful combination for probiotic delivery. International Journal of Food Microbiology, 141: 97–103.
● Ricci, G., Borgo, F., Ferrario, C., and Fortina, M. G. (2011). Cocoa powder as delivery medium for probiotic Lactobacillus strains. Advances in Microbiology,1: 1-6.
● Rokka, S., and Rantamäki, P. (2010). Protecting probiotic bacteria by microencapsulation: challenges for industrial applications. European Food Research and Technology, 231: 1-12.
● Shah, N.P.(2000). Symposium: prodiotic bacteria selective enumeration and survival in dairy foods. Journal of Dairy Science, 83: 894-907.
● Soukoulis, C., Lyroni, E., and Tzia, C. (2010). Sensory profiling and hedonic judgement of probiotic ice cream as a function of hydrocolloids, yogurt and milk fat content. Food Science and Technology, 43: 1351–1358.
_||_● Akalın, A.S., Unal, G., Dinkci, N., and Hayaloglu, A.A. (2012). Microstructural, textural, and sensory characteristics of probiotic yogurts fortified with sodium calcium caseinate or whey protein concentrate. Journal of Dairy Science, 95: 3617–3628.
● Akin, M.B., Akın, M.S., and Kırmacı, Z. (2007). Effects of inulin and sugar levels on the viability of yogurt and probiotic bacteria and the physical and sensory characteristics in probiotic ice-cream. Food Chemistry, 104: 93–99.
● Dols, J.A.M., Boon, M.E., Monachese, M., Changalucha, J., Butamanya, N., Varriano, S., Vihant, O., Hullegie, Y., van Tienen, A., Hummelen, R., and Reid, G. (2011). The impact of probiotic yogurt on HIV positive women in Tanzania. International Dairy Journal, 21: 575–577.
● Donkar, O.N., Nilmini, S.L.I., Stolic, P., Vasiljevic, T., and Shah, N.P. (2007). Survival and activity of selected probiotic organisms in set-type yogurt during cold storage. International Dairy Journal, 16: 1181–1189.
● Erdem, O., Gultekin-ozguven, M., Berktas, I., Ersan, s., Tuna, H.E., Karadag, A., and Ozcelik, B. (2013). Development of a Novel Synbiotic Dark Chocolate Enriched with Bacillus indicus HU36, Maltodextrin and Lemon Fiber: Optimization by Response Surface Methodology. Food Science and Technology. 56 (1): 187–193.
● Farnworth, E.R., Mainville, I., Desjardins, M.P., Gardner, N., Fliss, I., and Champagne, C. (2007). Growth of probiotic bacteria and bifidobacteria in a soy yogurt formulation. International Journal of Food Microbiology, 116: 174–181.
● Hoppert, K., Zahn, S., Jänecke, L., Mai, R., Hoffmann, S., and Rohm, H. (2013). Consumer acceptance of regular and reduced-sugar yogurt enriched with different types of dietary fiber. International Dairy Journal, 28: 1–7.
● Irkin, R., and Guldas, M. (2011). Evaluation of cacao-pudding as a probiotic food carrier and sensory acceptability properties. Acta agriculturae Slovenica, 97: 223-232.
● Institute of Standards and Industrial Research of Iran (2008). Probiotic yogurt - Characteristics and test methods. Standard No. 11325. [in Persian]
● Institute of Standards and Industrial Research of Iran (2008).Yoghurt-Characteristics and test methods. Standard No. 695. [in Persian]
● Institute of Standards and Industrial Research of Iran (2007). Milk and dairy products - Enumeration of colony forming units mold or yeast - colony count at 25 ° C in the plate. Standard No. 10154. [in Persian]
● Institute of Standards and Industrial Research of Iran (2007). Microbiology of food and animal feeding stuffs - Comprehensive method for counting coliforms - colony count method - national standard of 9263. [in Persian]
● Institute of Standards and Industrial Research of Iran (2006). Determination of acidity titratable and potentiometric ph in milk and milk products, 1st Edition, Standard No. 2852. [in Persian]
● Institute of Standards and Industrial Research of Iran (2005). Detection and enumeration of presumptive Escherichia coli in foods - most probable number technique, 1st Revision, 3rd Edition, Standard No. 2946. [in Persian]
● Institute of Standards and Industrial Research of Iran (2005). Microbiology of food and animal feeding stuffs - enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) - Test Method, Part I, using medium-range Culture medium Bard - Parker agar. Standard No. 1-6806. [in Persian]
● Institute of Standards and Industrial Research of Iran (2004). Milk and dairy products - raw milk specifications and test methods. Standard No. 164. [in Persian]
● Institute of Standards and Industrial Research of Iran (2002). Cheese and processed cheese - Determination of total dry matter (Reference method) - Test Method (revised). Standard No. 1753. [in Persian]
● Institute of Standards and Industrial Research of Iran (2002). Milk and dairy products - total count of colonies of microorganisms at 30 degrees Celsius. Standard No. 5484. [in Persian]
● Institute of Standards and Industrial Research of Iran (1991). Measurement Milk fat. Standard No. 366. [in Persian]
● Jayeola, C. O., Yahaya, L. E., and Igbinadolor, R. O. (2010). Cocoa powder supplementation in yoghurt production. Journal of Food Technology, 3: 82-85.
● Lollo P.C.B., de Moura, C.S., Morato, P.N., Cruz, A.G., and Castro, W.F. (2013). Corrigendum to “Probiotic yogurt offers higher immune-protection than probiotic whey beverage. Food Research International, 54: 923.
● Madhu, A.N., Amrutha, N., and Prapulla, S.G. (2012). Characterization and antioxidant property of probiotic and synbiotic yogurts. Probiotics and Antimicrobial Proteins, 4: 90-97.
● Marafon A.P., Sumi, A., Granato, D., Alcântara, M.R., Tamime, A.Y., and Nogueira de Oliveira, M. (2011). Effects of partially replacing skimmed milk powder with dairy ingredients on rheology, sensory profiling, and microstructure of probiotic stirred-type yogurt during cold storage. Journal of Dairy Science, 94: 5330–5340.
● Matto, J., Malinen, E., Suihko, M. L., Alander, M., Palva, A. and Saarela, M. (2004). Genetic heterogeneity and technological properties of intestinal bifidobacteria. Journal of Applied Microbiology. 97: 459–470.
● Possemiers, S., Marzorati, M., Verstraete, W., and Van de Wiele, T. (2010). Bacteria and chocolate, A successful combination for probiotic delivery. International Journal of Food Microbiology, 141: 97–103.
● Ricci, G., Borgo, F., Ferrario, C., and Fortina, M. G. (2011). Cocoa powder as delivery medium for probiotic Lactobacillus strains. Advances in Microbiology,1: 1-6.
● Rokka, S., and Rantamäki, P. (2010). Protecting probiotic bacteria by microencapsulation: challenges for industrial applications. European Food Research and Technology, 231: 1-12.
● Shah, N.P.(2000). Symposium: prodiotic bacteria selective enumeration and survival in dairy foods. Journal of Dairy Science, 83: 894-907.
● Soukoulis, C., Lyroni, E., and Tzia, C. (2010). Sensory profiling and hedonic judgement of probiotic ice cream as a function of hydrocolloids, yogurt and milk fat content. Food Science and Technology, 43: 1351–1358.
