A preliminary study on prevalence of Yersinia enterocolitica in beef, lamb and poultry at retails of Shahrekord
Subject Areas : Food Science and Technologyامیر Shakerian 1 , علی Sharifzadeh 2 , پیمان Aghanejad 3 , مهران Tajmir Riahi 4 , الهام Salehi 5
1 - Department of Food Hygiene, Faculty of Veterinary Medicine, Shahre-Kord Branch, Islamic Azad University, Shahre-Kord, Iran.
2 - Department of Pathobiology, Faculty of Veterinary Medicine, Shahre-Kord Branch, Islamic Azad University, Shahre-Kord, Iran
3 - Graduated of Veterinary Medicine, Shahre-Kord Branch, Islamic Azad University, Shahre-Kord, Iran.
4 - Graduated of Veterinary Medicine, Shahre-Kord Branch, Islamic Azad University, Shahre-Kord, Iran.
5 - Student of Veterinary Medicine Faculty, Shahre-Kord University, Shahre-Kord, Iran.
Keywords: poultry, lamb, Yersinia enterocolitica, beef,
Abstract :
Yersinia enterocolitica is a pathogenic organism that has recently emerged world-wide and its incidence is increasing. Human infection with Y.enterocolitica could cause diarrhea, abdominal pains, appendicitis like syndrome, vomiting, fever and septicemia. The main sources of human illness include pork, beef, milk vegetables, water and wild and domestic animals. This study was carried out on 300 meat samples including 100 beef, 100 lamb and 100 poultry samples at retail of Shahre-kord. The samples were transferred to PBS containing sorbitol. After 21 days of incubation at 40C, samples were cultured on CIN agar supplemented with CIN antibiotics. Putative colonies were confirmed by biochemical tests. Results showed that, 42 (14%) of samples including 4(4%) beef, 4(4%) lamb and 34(34%) poultry samples were contaminated with Y.enterocolitica. According to results of this study, intensive hygienic measures should be considered during slaughtering, storage and distribution of different kinds of meat.
Arnold, T., Neubauer, H., Nikolaou, K., Roesler, U. and Hensel A. (2004). Identification of Yersinia enterocolitica in minced meat: A comparative analysis of API 20 E, Yersinia identification kit and 16S rRNA- based PCR method. Journal of Veterinary Medicine, 51: 23-27.
Carter, G.R, Quinn, P.J., Carter, M.E. and Markey, B. (1994). Clinical Veterinary Microbiology, Wolf Company, 173: 210-221.
Lucero Estrada, C.S.M., Velazquez, L.D.C, Escudero, M.E., Favier, G.I., Lazarte, V. and Guzman, A.M.S.D. (2011). Pulsed field, PCR ribotyping and multiplex PCR analysis of Yersinia enterocolitica strains isolated from meat food in San Luis Argentina, Food Microbiology, 28: 21-28.
Mcnally, A., Cheasty, T. and Fearnely, C. (2004). Compration of the biotypes of Y.enterocolitica isolated from pigs, cattle, and sheep at slaughter and from human with yersiniosis in Great Britain: Letter Apply Microbiological, 39: 103-108.
Mauro, A., Lagana, P., Bruno, G., Micali, M., Minutol, E. and Delia, S. (2008). Isolation of Yersinia enterocolitica biotype 1 A from raw meat products. Journal of Preventive Medicine Hygiene, 49: 75-78.
Razavilar, V. (1999). Pathogenic microorganisms in foods and epidemiology of foodborne intoxi-cations, Tehran University Press, 2431: 96-103[In Farsi].
Sharifzadeh, A., Akhavan, M. and Zarasvandi Agha, S. (2004). Isolation of Yersinia enterocolitica and Listeria monocytogenes from raw and pasteurized milks supplied at dairies in Chaharmahal va Bakhtiary province, Iranian Journal of Food Science and Technology, 1(1): 15-20.
Siriken, B. (2004). The presence of Yersinia enterocolitica and other Yersinia species in ground beef in Aydin, Turkey. Turkish Journal of Veterinary Animal Science, 28: 489-495.
Soltan Dalal, M., Izadpour, F., Khalife gholi, M., Khatami moghadam, M. and Hejazi, H. (2004). A survey of prevalence of Yersinia in chicken and meat presented in butchers under supervision of Tehran university of Medical Sciences, 6th Iranian Congress of Microbiology, 16-18 February 2004- Tehran, Iran, 113.
Tomsson, L. (2000). Trends and source of zoonotic agent in animal feeding stuffs, food and man. The NorwagianZoonosisCenter Journal, 30: 12-17.
Trajkovic-Pavlovic, L.B., Popovic, M.B., Novakovic, B.D., Gusman-Pasterko, V.P., Jevtic, M.R. and Mirilov, J. M. (2007). Occurrence of Campylobacter, Salmonella, Yersinia enterocolitica and Listeria monocytogenes in some retail food products in Novi Sad. Center of European Public Health, 15: 167-171.