Evaluation of seed dormancy and artificial seed production in the medicinal plant Teucrium polium L.
Subject Areas : Agriculture and climate
Saideh Saadat
1
,
Ahmad Majd
2
,
Lotfali Naseri
3
,
Alireza Iranbakhsh
4
,
Morad Jafari
5
1 - Ph.D. Student, Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 - Professor, Department of Biology, North Tehran Branch, Islamic Azad University, Tehran, Iran
3 - Associate Professor, Department of Horticulture, Urmia University, Urmia, Iran
4 - Professor, Department of Biology, Science and Research Branch, Islamic Azad University,Tehran, Iran.
5 - Associate Professor, Department of Horticulture, Urmia University, Urmia, Iran
Keywords: Artificial Seed, Seed Germination, Seed Dormancy, Teucrium polium L.,
Abstract :
Teucrium polium L. is an important medicinal plant from the Lamiaceae which is endangered and its seed dormancy and poor germination are its main agricultural problems. In this research, the effect of 14 different treatments consisting of gibberellic acid, sulfuric acid, and chilling was studied based on a completely randomized design with three replications on breaking seed dormancy. There was a significant difference between different treatments. The best seed germination performance was observed in the seeds treated with sulfuric acid 98% for 15 minutes and gibberellic acid solution (1000 ppm) for 120 hours at 4°C, while the lowest was found in seeds soaked in water and kept at 4°C for two weeks and cultured in MS culture medium. The results of this research showed that the treatment of sulfuric acid and gibberellic acid along with cooling led to the highest percentage of seed germination, implying seed dormancy in these plants is a physical-physiological type. The results showed that artificial seeds with meristem origin were better than artificial seeds with somatic embryo origin under the conditions of growth room and refrigerator in terms of germination percentage, root and stem production. Therefore, with the mentioned methods, it is possible to solve the agricultural problem of this plant and prevent its extinction by producing artificial seeds and provide enough raw materials for the production of different medicines with the origin of this plant.
