Evaluation of the Antioxidant Activity of Dorema Aucheri Extract in Soybean Oil

Subject Areas : Microbiology

R. Mohammadi ¹ ( عضو باشگاه پژوهشگران جوان، دانشجوی کارشناسی ارشد، گروه علوم و صنایع غذایی، واحد اصفهان (خوراسگان)، دانشگاه آزاد اسلامی، اصفهان، ایران )
M. Fazel ² ( استادیار گروه علوم و صنایع غذایی، واحد اصفهان (خوراسگان)، دانشگاه آزاد اسلامی، اصفهان، ایران )
E. Khosravi ³ ( مربی گروه علوم و صنایع غذایی، واحد اصفهان (خوراسگان)، دانشگاه آزاد اسلامی، اصفهان، ایران )

Received: 2016-11-16 Accepted : 2016-11-16 Published : 2016-12-21

Keywords: Antioxidant Activity, Dorema Aucheri Extract, Oven Test, Phenolic Compounds, Soybean Oil,

Abstract :

Introduction: Due to the recognized antioxidant activities of compounds present in herbs and spices, there has been considerable interests concerned with this subject and the biological systems. This research work has been carried out in order to investigate the antioxidant activity of Dorema Aucheri plant and also the application of its ethanolic extract to refined bleached and deodorized soyabean oil to retard the rate of oxidation. Materials and Methods: The effects of water and 96% ethanol as solvents on extracting efficiencies of phenolic compounds and flavonoids of the sample were investigated. The antioxidant activities of the samples with different added concentrations of the extract using DPPH radical scavenging and Ferric-reducing power were determined and evaluated. Results: The results showed that the aqueous extract has the highest extraction rate as compared to the ethanolic extract, however the highest concentrations of phenolic compounds (21.73 ± 0.89 mg GAE/g) and flavonoid (14.99 ± 0.60 mg QE/g sample), and the lowest IC50 concerned with DPPH (2.05 mg extract/ mg DPPH) and the highest rate of Ferric reducing power (14.13 ± 0.06 mM Fe+2/mg) were related to the ethanolic extract. The results of oven test also indicated that the ethanolic extract at certain concentrations could control the rate of oxidation. Conclusion: The results of this investigation suggested that the Dorema Aucheri ethanolic extract might be employed as an antioxidant. Therefor one might introduce Dorema aucheri as a good source of natural antioxidant due to the presence of phenolic compounds. 

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