Optimization of Canola Oil Extraction by Ultrasound Waves and Response Surface Methodology

Subject Areas : Microbiology

F.  Jalili ¹ (دانش آموخته کارشناسی ارشد علوم و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران)
S. M.  Jafari ² (دانشیار دانشکده صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران)
Z.  Emamjomeh ³ (استاد گروه صنایع غذایی، دانشگاه تهران، تهران، ایران)
M.  Kashaninejad ⁴ (استاد دانشکده صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران)
M.  Ganjeh ⁵ (دانشجوی دکترای مهندسی مواد و طراحی صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران)

Received: 2017-09-05 Accepted : 2017-09-05 Published : 2017-09-23

Keywords: Canola, Extraction, Gas Chromatography (GC), Response Surface Methodology, Ultrasound Waves,

Abstract :

Introduction: Canola seed is one of the most important oil seed with high ratio of oil andhigh nutritional value and is economically recommended for cultivation and oil extraction.Extraction with ultrasound waves as a nondestructive and novel method might be employed.Therefore the aim of this research is optimization of extraction of canola oil by ultrasoundwaves and RSM.Materials and Methods: In this project, Response Surface Methodology was employed foroptimization of canola oil extraction by ultrasound waves using hexane andhexane/isopropanol as solvents by the ratio of 3:2 (v/v). The influence of extractiontemperature in the range of 35, 45 and 55℃, ultrasound treatment time for 30, 60 and 90minutes and ratio of solvent to canola in three levels of 5, 10 and 15 ml/g were analyzed byBox-Behnken Design (BBD) based on extracted oil percent. The fatty acid compositions ofthe extracted oils using ultrasound-assisted method and soxhlet method employing gas liquidchromatography were determined and compared.Results: According to the high values of correlation coefficients of selected models optimizedfor hexane solvent extraction methods (R2 =0.93) and hexane-isopropanol solvent mixture(R2 =0.97) and no significant lack of fit tests (P>0.05) selected models might be useful topredict the extraction percentage of canola oil.Conclusion: The results showed that the optimum conditions for canola oil extraction byhexane (22.39 %) and combined hexane/isopropanol (30.66 %) were respectively atultrasound treatment times of 69.5 and 87 minutes, temperatures of 55 and 55 ℃ and ratio ofsolvent to canola of 6.39 and 9.12 (%v/w). Extraction efficiency was significantly improvedby ultrasound as compared to the soxhlet extraction. Fatty acid compositions of the canolaoils were not significantly affected by the application of ultrasound (P> 0.05). Thereforeultrasound might be regarded as a simple and rapid method to improve the extraction of oilwithout affecting the quality of the fatty acids present.

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