Effect of Hydrogen Peroxide and 24-Epibrassinolide on Secondary Metabolites and Phytochemistry of Cumin (Cuminum cyminum L.) under Drought Stress
Subject Areas : Agriculture and climate
Nasim Roodbari
1
,
Hossein Abbaspoor
2
1 - Department of Biology, Kah.C., Islamic Azad University, Kahnooj, Iran
2 - Department of Biology, NT.C., Islamic Azad University, Tehran, Iran
Keywords: 24- epibresino steroids, Hydrogen peroxide, Protein, Drought stress, Photosynthetic pigments, Cumin, and phenol.,
Abstract :
Two signaling materials, namely hydrogen peroxide and 24-epi brassinosteroids, have significant biological effects on plant growth, including increasing plant tolerance to environmental stresses. In the present study, the effect of drought stress and its interaction with H2O2 and 24-epibrassinolide on protein content, sugars, essential oil percentage, photosynthetic pigments (chlorophyll a, b), auxiliary pigments (carotenoids, phenols, and flavonoids) were investigated. For this purpose, an experiment was conducted based on a factorial design in a completely randomized design with three repetitions in the research greenhouse of the Agricultural Research, Education and Natural Resources Centre of Southern Kerman, and the plants under went drought stress treatment at three levels of irrigation 100% (control), 75% (mild stress) and 50% field capacity (severe stress) in the fifth week after germination (the beginning of reproductive growth), Which successively, in 2 stages, first three days before stress, and 15 days later, spraying hydrogen peroxide on the plants was applied at concentrations (0, 0.5 and 1 mM) and 24-epi brosinosteroids (0, 0.5 and 1 mM). With increasing levels of stress, essential oil percentage, sugar soluble, carotenoids, phenolic compounds, flavonoids, the shoot increased, and protein of aerial organ and chlorophyll decreased. The use of H2O2 and 24-epibrassinolide in drought stress conditions had a positive and significant effect on the tested traits. The levels of protein, chlorophyll a and b increased by concentrations of H2O2 and 24-epibrassinolide in the same levels of stress. As a result, by spraying 24-epibrassinolide at 1 mM, chlorophyll a reached its highest level (mL-190/10). Spraying 24-epibrassinolide under severe stress conditions increased flavonoids of the aerial organ by 1.58 (Observance g) and decreased essential oil percentage (2.44%). In general, it can be said that drought stress reduced the ability of photosynthesis and plant production. Spraying hydrogen peroxide and 24-epibrassinolide was able to improve the photosynthetic system and resistance of the cumin plant to stress.
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