Effect of Rosa damascena Mill. Against CCl4- Liver Injury in Adult Male Wistar Rats

Subject Areas : Journal of Animal Biology

Fatemeh Zahra Dibaei ¹ (Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran)
Akram Eidi ² (Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran)
Pejman Mortazavi ³ (Department of Pathology, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran.)

Received: 2023-12-08 Accepted : 2024-02-13 Published : 2024-08-06

Keywords: Damask rose, Rosa damascena Mill., Liver, Carbon tetrachloride, Rat,

Abstract :

Damask rose (Rosa damascena Mill.) belongs to the Rosaceae family which grows in many countries including Iran. The dried flowers of rose are used medicinally and are believed to alleviate conditions such as colds, chronic bronchitis, asthma, skin diseases, cancer, wounds, injuries, wrinkles, infections, as well as constipation. This study was carried out to investigate the protective effect of ethanolic extract of rose on carbon tetrachloride (CCl4)-induced liver toxicity in adult Wistar rats. Animals have divided into 8 groups including normal control, liver damage control (CCl4, twice a week), normal experimental (rose flower extract at doses 50, 100, 200 mg/kg), liver damage experimental (CCl4 + rose flower extract at doses 50, 100, 200 mg/kg) and treated for 28 days. After 28 days, animals were anesthetized and blood was collected from the heart. Levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) were measured in the serum. The activity of superoxide dismutase (SOD) and the level of malondialdehyde (MDA) were measured in the liver homogenate.The result of the present study showed that rose extract reduced the levels of liver enzymes, including ALT, AST, and ALP. Also, administration of rose extract increased SOD and decreased MDA levels. These results indicate that rose extract is effective in protection against liver injury and oxidative stress caused by CCl4.

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