Bacterial community structure in saline sediments from hypersaline wetland in south of Halghe Dare hills, Alborz province

Subject Areas : Environmental Microbiology

Seyed Sina Seyedpour Layalestani ¹ , Mahmoud Shavandi ² , Azam Haddadi ³ , Mohammad Ali Amoozegar ⁴ , Seyed Mohammad Mehdi Dastgheib ⁵

1 - Ph.D student, Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran.
2 - Assisstant Professor, Microbiology and Biotechnology Group, Research Institute of Petroleum Industry
3 - Assistant Professor, Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran.
4 - Professor, Department of Microbiology, College of Science, University of Tehran, Tehran, Iran.
5 - Assistant Professor, Microbiology and Biotechnology Research Group, Research Institute of Petroleum Industry, Tehran, Iran.

Received: 2020-09-05 Accepted : 2020-10-01 Published : 2020-11-21

Keywords: Bacterial diversity, Hypersaline wetland, Halotolerant and halophilic species, Next generation sequencing,

Abstract :

Background & Objectives: Survey of bacterial community structure in hypersaline ecosystems and identification of novel halophilic species can be very important from biotechnological and ecological aspects. In this study, we survey bacterial community structure in sediments from saline wetland in south of Halghe Dare hills as one of the hypersaline ecosystems in Alborz province. Materials & Methods: This cross-sectional study was performed by sampling from saline wetland in south of Halghe Dare hills in June 2018. Isolation of heterotrophic bacteria was conducted using R2A agar medium. After differentiation of isolates based on morphological and biochemical characteristics, identification and phylogenetic relationships analysis of selected isolates were performed by 16S rRNA gene sequencing and analysis using NCBI databases and bioinformatics softwares. The Illumina next-generation sequencing was also applied to survey bacterial diversity by cultivation-independent method. Results: Isolates included 13 species belonging to 8 genera including Bacillus (31.25%), Halomonas 25%, Gracilibacillus (12.50%), Virgibacillus (6.25%), Streptomyces (6.25%), Nitratireductor (6.25%), staphylococcus (6.25%) and Planococcus (6.25%). Illumina sequencing showed that Aneurinibacillus migulanus and Paenibacillus polymyxa were dominant species insoil sample. Conclusion: The results showed that the microbial population of the studied wetland is similar to the community of the wetlands reported in other parts of the world and dominated by halotolerant and halophilic species. Presence of various bacterial species and some probable novel taxonomic groups in saline wetland in south of Halghe Dare hills presents a new genetic and microbial source for future studies.  

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