List of Articles Bacterial diversity

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  1 - Identification of the bacterial microflora of fresh edible yellow mealworm larvae (Tenebrio molitor L.)
  s. mohammad salim sohrab imani m. abdi godarzi n. hasan zadeh v. zarin niya
  The edible insects are consumed as an alternative animal protein source by most Asian countries. Since there is a limited evidence for their safety particularly from microbiological aspects, an attempt was made to assess the microbial population of Tenebrio molitor L. s More

  The edible insects are consumed as an alternative animal protein source by most Asian countries. Since there is a limited evidence for their safety particularly from microbiological aspects, an attempt was made to assess the microbial population of Tenebrio molitor L. samples in laboratory conditions. Primary stocks were purchased from a local market, in Sari- a city in north of Iran. Microbial samples were prepared from both body surfaces and guts of insect. Samples were individually and cultured on nutrient agar (NA), incubated at taken 270C for 24-72h. Distinguished colonies were isolated and purified. Based on phenotypic characteristics, hypersensitive response (HR) on geranium leaves, as well as 16S rRNA gene sequencing analysis, the isolates were categorized into two groups. The pathogenic isolates were identified as Bacillus cereus, Staphylococcus sp, Pseudomonas aeruginosa, Enterobacter asburiae, Bacillus firmus and Serratia marcescens. Whereas, the nonpathogenic bacteria were assigned as Enterobacter cloacae and Bacillus thuringiensis. Undoubtedly, the presence of pathogenic microbes in the microflora of mealworm larvae by direct and indirect consumption of insect may pose a threat to human and animal health. These findings suggest an implementation of certain processing methods in order to decrease or eradicate risks of microbial contamination of diets using natural insects. Manuscript profile
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  2 - Bacterial diversity of Apis florea F. (Hym., Apidae) in Iran
  Sh. Parichehreh G. H. Tahmasbi A. Sarafrazi S. Imani N. Tajabadi
  Apis florea F. and Apis mellifera L. are two honey bee species in Iran. Distribution of Apis florea starts from Paveh in Kermanshah province (located in west of Iran) and ends to Sistan-Baluchestan province (located in south east of Iran). The current study was carried More

  Apis florea F. and Apis mellifera L. are two honey bee species in Iran. Distribution of Apis florea starts from Paveh in Kermanshah province (located in west of Iran) and ends to Sistan-Baluchestan province (located in south east of Iran). The current study was carried out by collecting 1400 worker bees from 14 different colonies located in southern regions of Iran. The samples were kept in sterile test tubes containing normal saline. The specific media were used to isolate Lactobacillus bacteria from digestive tracts of the bees. Biochemical tests and DNA extraction were done to identify the colonies. In addition, the colonies were characterized by sequencing 16S rRNA gene using specific primers (27F and 1492R). Since bacterial diversity in digestive tracts is of the approaches for classifying insects from phylogenetic point stand, sequencing was done on 43 colonies of bacteria. The results showed that eight isolates were related to three species (Lactobacillus kunkeei, Lactobacillus plantarum and Lactobacillus apis). Basis on the results, the dwarf honey bee in Iran phylogenetically clustered in five distinct clades in terms of bacterial diversity in digestive tracts. The clades were: 1): Roudan, Bandar-Abbas, Iranshahr 2) Jiroft, Kahnuj, Bushehr, Ahwaz, Dehloran 3) Gachsaran 4) Jahrom, Fasa, Behbahan and 5) Qeshm population. Furthermore, the results indicated that Lactic acid bacterias found in digestive tracts depends on nectar and pollen feeding by this wasp in the geographically different localities. Manuscript profile
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  3 - Study of Bacterial Diversity in Poultry Gut Using Denaturing Gradient Gel Electrophoresis
  V.B. Ahir K.M. Singh A.K. Tripathi R.A. Mathakiya S.J. Jakhesara P.G. Koringa D.N. Rank M.K. Jhala C.G. Joshi
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  4 - Bacterial community structure in saline sediments from hypersaline wetland in south of Halghe Dare hills, Alborz province
  Seyed Sina Seyedpour Layalestani Mahmoud Shavandi Azam Haddadi Mohammad Ali Amoozegar Seyed Mohammad Mehdi Dastgheib
  Background & Objectives: Survey of bacterial community structure in hypersaline ecosystems and identification of novel halophilic species can be very important from biotechnological and ecological aspects. In this study, we survey bacterial community structure in se More

  Background & Objectives: Survey of bacterial community structure in hypersaline ecosystems and identification of novel halophilic species can be very important from biotechnological and ecological aspects. In this study, we survey bacterial community structure in sediments from saline wetland in south of Halghe Dare hills as one of the hypersaline ecosystems in Alborz province. Materials & Methods: This cross-sectional study was performed by sampling from saline wetland in south of Halghe Dare hills in June 2018. Isolation of heterotrophic bacteria was conducted using R2A agar medium. After differentiation of isolates based on morphological and biochemical characteristics, identification and phylogenetic relationships analysis of selected isolates were performed by 16S rRNA gene sequencing and analysis using NCBI databases and bioinformatics softwares. The Illumina next-generation sequencing was also applied to survey bacterial diversity by cultivation-independent method. Results: Isolates included 13 species belonging to 8 genera including Bacillus (31.25%), Halomonas 25%, Gracilibacillus (12.50%), Virgibacillus (6.25%), Streptomyces (6.25%), Nitratireductor (6.25%), staphylococcus (6.25%) and Planococcus (6.25%). Illumina sequencing showed that Aneurinibacillus migulanus and Paenibacillus polymyxa were dominant species insoil sample. Conclusion: The results showed that the microbial population of the studied wetland is similar to the community of the wetlands reported in other parts of the world and dominated by halotolerant and halophilic species. Presence of various bacterial species and some probable novel taxonomic groups in saline wetland in south of Halghe Dare hills presents a new genetic and microbial source for future studies.   Manuscript profile
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  5 - The effect of altitude on the bacterial diversity and abundance of the soil samples of Kazem Khan Mountain of saline Lake Urmia
  Fatemeh ghafarnejad mogadam Mahmoud Shavandi Azam Haddadi Mohammad Ali Amoozegar
  10.30495/jmw.2022.1966410.2033
  Background & Objectives: Considering the critical conditions of Lake Urmia, identifying bacteria with the ability to live in extreme environments is valuable in terms of microbial applications and tolerance of the existing biological conditions, and it helps us to b More

  Background & Objectives: Considering the critical conditions of Lake Urmia, identifying bacteria with the ability to live in extreme environments is valuable in terms of microbial applications and tolerance of the existing biological conditions, and it helps us to better understand the surrounding environment. In this study, the most abundant microbial branch in the soil samples obtained from three different heights of 10, 150 and 250 meters of Qale Kazem Khan Mountain, which is located on the shore of a very salty lake in Urmia, has been investigated. Materials & Methods: The soil samples were collected to identify and classify Proteobacteria    subgroups using 16S rRNA sequencing using Next Generation Sequencing (NGS) as well as FLASH genetic software and UCHIME algorithm to identify the obtained sequences. Results: Altitude change affects the abundance of Alphaproteobacteria and Betaproteobacteria. The abundance percentage of Alphaproteobacteria has a direct relationship with altitude, while the abundance percentage of Betaproteobacteria increased with the decrease in altitude. Conclusion: in the overview the percentage of Proteobacteria abundance the samples has an inverse relationship with the increase in height, whereas in the separate examination of the microbial groups, a significant relationship between the increase and decrease in abundance and the height of sampling is observed. Also, two unknown and unclassified genera in the  Deltaproteobacteria order were also identified which a very high frequency percentage (18-27%) among the data had related to the three samples.   Manuscript profile