The Survey of anticancer and anti Microbial properties of Rosmarinus officinalis L, Peganum harmala L and Coffee Extracts on cell viability of Cancer cells and Pathogenic Bacteria in Foods

Subject Areas : Food Microbial Contamination

Ali Sharifzadeh ¹ , faranak aali ² , sayeh vahhabi ³

1 - IAU, Shahrekord
2 - 2-Biotechnology Research Center, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
3 - Department of veterinary medicine, Faculty of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

Received: 2020-01-01 Accepted : 2020-07-03 Published : 2021-05-22

Keywords: Pathogenic bacteria, coffee, Rosemary, Cancer Cell Line, Peganum,

Abstract :

Despite significant advances in radiation therapy and cancer treatments in the past 30 years, resistance to chemotherapy is a major obstacle in the recovery of patients with cancer. Resistance to chemotherapy drugs inhibits the recovery process. This study aims to evaluate the anticancer activity of the hydroalcolic extracts of Rosemary, Peganum and Coffee on growth inhibition of MCF-7 cell line and determine the rate of growth inhibition of pathogenic bacteria isolated from food.We examined cytotoxicity effects of three different concentrations (60,120,250 μg/ml) of Rosemary, Peganum and Coffee extracts on MCF-7and HDF cell lines. . The standard MTT assay was performed to estimate cell viability after treatment by Rosemary, Peganum and Coffee extracts. The extracts were also used with MIC on Staphylococcus aureus and E. coli bacteria isolated from food.The results of the MTT assay showed that the Rosemary, Peganum and Coffee extracts had time-and concentration-dependent anticancer activities on the MCF-7cell line compared to HDF cells statistically significant (p