List of Articles Pathogenic bacteria

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  1 - Biosensors Application in Analysis of Environmental contaminants from Agricultural Industries
  Marzieh Hosseini Nezhad Saeed Samadi
  Water contamination and environmental pollution is considered as a main concern in global healthissues. Considering the large number of chemical and microbial contaminants released to theenvironment, in particular those from agricultural origin, effective tools of detec More

  Water contamination and environmental pollution is considered as a main concern in global healthissues. Considering the large number of chemical and microbial contaminants released to theenvironment, in particular those from agricultural origin, effective tools of detection are required forprevention of challenges involved in health and safety. Biosensors offer rapid and effective detectionoptions to control biological hazards. These are recognized as beneficial devices in providing accurate,sensitive and rapid analytical results in quantitative and qualitative detection of pollutants. This paperis summarizes the advances in development and application of biosensors for environmental analysisand quantification of contaminants initiated from agricultural procedures Manuscript profile
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  2 - Study of antioxidan and antimicrobial effects of aqueous and alcoholic extract of plant ( Lawsonia inermis, punica granatum, walnut, Myrtus) on gram positive and gram negative bacteria
  simin khosravi zahra rezayatmand mozhgan ghiasian
  Recently , due to the resistance of bacterial pathogens to the detection of new antimicrobials, much attention has been paid. In the present study, the antimicrobial properties of four extracts of leafy leafy leaves, henna leaves, walnut shells and pomegranate peel on t More

  Recently , due to the resistance of bacterial pathogens to the detection of new antimicrobials, much attention has been paid. In the present study, the antimicrobial properties of four extracts of leafy leafy leaves, henna leaves, walnut shells and pomegranate peel on three gram-positive bacteria, Staphylococcus aureus, Staphylococcus epidermidis and Bacillus subtilis, and two gram-negative bacteria of Escherichia coli and Pseudomonas aeruginosa were studied. it placed. Antimicrobial effect of the alcoholic and aqueous extract of these plants was studied by diffusion and well discarding method by measuring the non-growth aura's diameter and the minimum inhibitory concentration was investigated by ELISA method. The results show that in two methods, diffusion discs and wells in alcoholic and aqueous extracts have the highest effect on Staphylococcus epidermidis bacterium and have the least effect on E. coli bacteria. The minimum amount of MIC for alcoholic extract is 31.25 and MBC 54.25 mg / ml, which is related to Escherichia coli bacteria. The results measurements of total phenolic compounds, flavonoids and tannins also indicate that the highest amount of this product is in the concentration of 500 mg / ml of the extract of plants. Also, the amount of antioxidant activity in the alcoholic extract is about twice as much as the aqueous extract. The results of this study indicate that the combination of extracts of plants, especially alcoholic extract, has a more antibacterial effect than the aqueous extract, which can be due to the presence of more antibacterial compounds in the extract. Manuscript profile
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  3 - An overview of fish pathogenic bacteria and the effect of medicinal plants against
  mehdi jahane Reza Rouhani maryam bigomi zahra bigomi Fatima Farzaneh saeide saeidi
  10.30495/qafj.2022.1964290.1033
  Abstract:Indiscriminate use of antibiotics to treat bacterial diseases in aquaculture has caused the emergence of drug resistance in bacterial strains and reduced the effectiveness of drugs, in addition, it has caused the accumulation of antibiotics in the body of fish More

  Abstract:Indiscriminate use of antibiotics to treat bacterial diseases in aquaculture has caused the emergence of drug resistance in bacterial strains and reduced the effectiveness of drugs, in addition, it has caused the accumulation of antibiotics in the body of fish and fish consumers. Therefore, it seems necessary to replace less harmful substances, including plant products. The aim of this study is to review fish pathogenic bacteria and the effect of medicinal plants on them.Materials and methods:In this study, valid scientific articles indexed in ISI, SID, PubMed, PubMed Central, Scopus, Web of Science databases were examined using Persian keywords drug resistance, fish pathogenic bacteria, medicinal plants.Results:A review of the studies shows that medicinal plants and their compounds are able to destroy fish pathogenic bacteria and infections caused by them in different concentrations.Discussion:According to the obtained results, medicinal plants can be suggested for the treatment of infections caused by fish pathogenic bacteria. Manuscript profile
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  4 - Effect of catheterization on prevalence of urinary infections
  Fatemeh Mohammadzadeh Hamed Zarei سحر هنرمند جهرمی
  .. Urinary tract infection is one of the most important nosocomial infections that is often caused by catheterization. Europathogenic bacteria can potentially cause urinary tract infections by forming biofilms in catheters. The aim of present study was to investigate th More

  .. Urinary tract infection is one of the most important nosocomial infections that is often caused by catheterization. Europathogenic bacteria can potentially cause urinary tract infections by forming biofilms in catheters. The aim of present study was to investigate the formation of Klebsiella pneumoniae biofilm from catheter-associated urinary tract infection. The study was performed on 110 urine samples of patients who referred to the clinical laboratory of Milad Hospital, Tehran. Patients were divided into two groups with catheter-associated and non-catheter- associated urinary tract infections. Isolation and identification of Klebsiella pneumoniae were performed by biochemical tests and biofilm formation by plate microtiter method. 70 and 40 samples were related to catheter- associated and non-catheter- associated infections, respectively. 60% of Klebsiella pneumoniae isolates from associated with urinary tract infections due to catheterization were strong biofilm, 26.7% moderate and 13.3% weak. 33.3% of Klebsiella pneumoniae isolates associated with urinary tract infection without catheter formed strong biofilm, 36.6% moderate and 23.3% weak biofilm and 2 isolates did not form any biofilm. The use of catheters in hospitalized patients increases the risk of urinary tract infections, which depends on various factors such as the duration of catheterization, type of catheter, and ability to form a biofilm of uropathogenic bacteria. The power of bacterial biofilm formation during catheterization increases the risk of infection and leads to antibiotic resistance of isolates and lack of proper treatment of patients. Manuscript profile
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  5 - Investigating the antibacterial effect of polar, semi-polar and non-polar fractions of Sarhargol plant extract (Echinacea purpura) on some bacteria causing infection in laboratory conditions
  behboud jafari
  ازجمله مشکلات شایع در دنیای پزشکی مسئله مقاومت باکتری‌ها در برابر آنتی‌بیوتیک‌ها بوده است و با توجه به وجود ترکیبات بیولوژیکی فعال در گیاه سرخارگل این گیاه دارای قابلیت ضد باکتریایی قابل‌ملاحظه‌ای می باشد. هدف از این مطالعه بررسی اثر آنتی‌باکتریال فراکسیون‌های قطبی، نیم More

  ازجمله مشکلات شایع در دنیای پزشکی مسئله مقاومت باکتری‌ها در برابر آنتی‌بیوتیک‌ها بوده است و با توجه به وجود ترکیبات بیولوژیکی فعال در گیاه سرخارگل این گیاه دارای قابلیت ضد باکتریایی قابل‌ملاحظه‌ای می باشد. هدف از این مطالعه بررسی اثر آنتی‌باکتریال فراکسیون‌های قطبی، نیمه قطبی و غیرقطبی عصاره گیاه سرخارگل بر روی برخی از میکروب های عامل عفونت در شرایط آزمایشگاهی می باشد. در این مطالعه ابتدا عصاره های متانولی ، کلروفرمی و هگزانی گیاه سرخارگل تهیه شد و تاثیر غلظت های مختلفی از عصاره ها مورد بررسی قرار گرفت. تمامی آزمایش ها با روش های انتشار چاهک و میکروتیتر پلیت بر روی سویه های استاندارد باکتری های استافیلوکوکوس اورئوس، اشریشیاکلی، سودوموناس آئروژینوزا و استرپتوکوکوس موتانس انجام گرفت. یافته ها نشان داد که اثر بازدارندگی عصاره های مختلف گیاه سر خارگل بر روی باکتر های گرم مثبت بیشتر از باکتری های گرم منفی است. همچنین اثر مهاری عصاره ی متانولی نسبت به عصاره های کلروفرمی و هگزانی بیشتر می باشد که در بین باکتر های مورد آزمایش استرپتوکوکوس موتانس و سودوموناس آئروژینوزا به ترتیب بیشترین و کمترین حساسیت را داشتند. نتایج حاصله بیانگر آن است که عصاره گیاه سرخارگل دارای اثر ضد باکتریایی است. لذا این عصاره میتواند گزینه مناسبی بر حسب مطالعات آینده تحت شرایط آزمایشگاهی جهت تهیه ی داروهای ضد باکتریایی باشد. Manuscript profile
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  6 - Evaluation of Anti-Bacterial Activity of Lavandula angustifalia Alcoholic and Aquatic Extracts on some Pathogenic Bacteria.
  Mehdi Ghiami rad alireza lotfi
  Lavender with the scientific name Lavadul angustifelia belongs to the mint family, and the extract and essence of this plant are widely used in traditional medicine. This study was conducted with the aim of investigating the antibacterial effects of alcoholic and aqueou More

  Lavender with the scientific name Lavadul angustifelia belongs to the mint family, and the extract and essence of this plant are widely used in traditional medicine. This study was conducted with the aim of investigating the antibacterial effects of alcoholic and aqueous extracts of lavender on some pathogenic bacteria. First, alcoholic and aqueous extracts of lavender were prepared by Soxhlet method, and then concentrations of 100 to 0.39% of this extract were prepared and their effect on standard strains of bacteria Bacillus subtilis, Streptococcus mutans, Listeria monocytogenes, Yersinia enterocolitica and Salmonella enteritidis determined by well diffusion method. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the extracts on the studied bacteria were measured by the micro-titer plate method with the reduction of resazurin dye. The results showed that the alcoholic and aqueous extracts of Lavander have a significant inhibitory effect on all five tested bacteria. By increasing the concentration of the extracts, their effect on the studied bacteria increased. Also, both extracts (aqueous and alcoholic) had a greater effect on gram-positive bacteria, so that both Lavender extracts showed the greatest effect on Bacillus subtilis and the least effect on Salmonella enteritidis. The inhibitory and lethality effects of the alcoholic extract was higher than that of the aqueous extract. Alcoholic and aqueous extracts of Lavandula angustifalia have significant inhibitory effects on the growth of pathogenic bacteria, especially gram-positive bacteria, so they can be used to against diseases caused by these bacteria. Manuscript profile
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  7 - Phytochemical characteristics and antimicrobial effect of Ziziphora clinopodioides and Coriandrum Sativum seed essential oils and their combination on some food borne pathogenic bacter
  S. Abbaszadeh M. Kargozari H. Gandomi NasrAbadi
  10.30495/jfh.2019.543640
  Essential oils and their components have known antibacterial effects. In this study the major components of essential oils of Coriandrum sativum seeds and aerial parts of Ziziphora clinopodioides were identified by means of GC-MS and the effect of different concentratio More

  Essential oils and their components have known antibacterial effects. In this study the major components of essential oils of Coriandrum sativum seeds and aerial parts of Ziziphora clinopodioides were identified by means of GC-MS and the effect of different concentrations of them alone and in combination were then investigated in vitro to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against some foodborne pathogenic bacteria including Escherichia coli O157:H7, Staphylococcus aureus, Salmonella Typhimurium, Listeria monocytogenes and Bacillus cereus using broth micro-dilution method.  The most principle compounds composing Coriander seed essential oil (CEO) were Linalool L,γ-Terpinene, α-Pinene, Geraniol acetate and cymene. Thymol, α-Terpineol, Carvacrol, Linalool L and γ-Terpinene were the main chemical compounds found in Ziziphora essential oil (ZEO). Considering the results of MIC and MBC, B. cereus was the most sensitive (MIC 500ppm, MBC 1000ppm) and S. Typhimurium was the most resistant species (MIC 2000ppm and MBC 5000ppm) against CEO.ZEO also showed more antimicrobial effect against Gram positive bacteria Staphylococcus aureus, Listeria monocytogenes and Bacillus cereus (MIC 500ppm) compared to Gram negative bacteria of E. coli O157:H7andS. Typhimurium (MIC 1000ppm). The results of the evaluation of the combination effect of aforementioned EOs indicated that ZEO+CEO could synergistically suppress the growth ofS.aureus (MIC 125+250 ppm, 250+125 ppm). The results of this study showed that Ziziphora and Coriander EOs when used in combination are more effective against Gram+ bacterial growth especially against S. aureus which is an important bacterial  Manuscript profile
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  8 - Investigating the antimicrobial properties of edible film of polylactic acid-Iranian tragacanth gum along with the essential oil of Zygophyllum eichwaldi on the microbial spoilage of ostrich thigh meat at refrigerator temperature
  Azin Kiakojori peiman ariaii Roya Bagheri Mahro Esmaeili
  10.30495/jfh.2023.1989373.1406
  The present study was conducted with the aim of investigating the effects of PLA- Iranian tragacanth gum composite film with Zygophyllum eichwaldi essential oil on the microbial characteristics of ostrich thigh meat stored at 4°C. For this purpose, first, 5 film tre More

  The present study was conducted with the aim of investigating the effects of PLA- Iranian tragacanth gum composite film with Zygophyllum eichwaldi essential oil on the microbial characteristics of ostrich thigh meat stored at 4°C. For this purpose, first, 5 film treatments including: PLA, PLA+ gum and PLA+ gum with concentrations of 0.5%, 1.5% and 1.5% essential oil to determine the microbial properties of the film and then the effect of the mentioned films along with the control treatment (without film) was evaluated on the microbial factor (total bacterial count, psychrophilic bacteria, Enterobacteriaceae and lactic acid) in the ostrich fillet samples tested on days 0, 4, 8, 12 and 16 and the resulting data were SPSS software was analyzed. According to the results, adding essential oil to the film increased the antimicrobial activity of the films, and with increasing concentration, better results were observed (P<0.05) and the antimicrobial activity against gram-positive bacteria was higher than gram-negative bacteria. The results of the second part of this study showed that the samples packed with composite films containing essential oil showed lower (P<0.05) microbial changes than the control samples without essential oil during the study, and the film containing 0.5 and 1% of essential oil had the best effects compared to other groups (P<0.05). Therefore, the use of PLA- Iranian tragacanth gum film containing Zygophyllum eichwaldi essential oil in the packaging of ostrich meat has a very favorable effect in controlling the microbial characteristics effective in meat spoilage and increases its shelf life. Manuscript profile
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  9 - Evaluation of Antibacterial Effect of Zinc Oxide Nanoparticles Synthesized by Wet-chemically Method on Multidrug-resistant Uropathogenic Bacteria
  Maryam Bagheri Mohammad Gholipour Monir Doudi Ali Mohammad Ahadi Gholam Reza Amiri
  10.22034/jna.2021.1930086.1255
  Zinc oxide (ZnO) nanoparticles have been attracted for their antibacterial effects along with their safety, suitable physicochemical properties, and cost effectivity. The aim of this study was to synthesize ZnO nanoparticles and investigate their antibacterial effects o More

  Zinc oxide (ZnO) nanoparticles have been attracted for their antibacterial effects along with their safety, suitable physicochemical properties, and cost effectivity. The aim of this study was to synthesize ZnO nanoparticles and investigate their antibacterial effects on multidrug resistant (MDR) uropathogenic bacteria. ZnO nanoparticles were constructed by wet chemical method with inhibitor. Structural and optical evaluation of zinc oxide nanoparticles was performed by Ultraviolet absorption spectroscopy, X-ray diffraction (XRD), and Tunneling microscopy. Uropathogenic bacteria were isolated from the patient’s pediatric urine and identified through biochemical testing. Antibiotic resistance pattern of the isolates was determined by disk diffusion method and the antibacterial activity of ZnO nanoparticles was detected by pour plate method at the concentrations of 0.0125, 0.025, 0.05, 0.1, 0.2, 0.4, and 0.8 g/l. The produced ZnO nanoparticles had a hexagonal shape with the crystal size of 15 nm and an optical size of 3 nm. The minimum effective concentration of these nanoparticles that inhibited the bacterial growth was 0.05 g/l. E.coli was generally found to be the most frequent cause of UTI in children in Isfahan, in the center of iran, with the mean prevalence of 64%. Most cases of resistance were related to Ampicillin, Amoxicillin, Tetracycline and the highest sensitivity was related to Chloramphenicol, Gentamicin, Trimethoprim/Sulfamethoxazol. The results of the present study, in accordance to the previously approved safe characteristics of ZnO nanoparticles, concentration of 0.05 g/l is proposed to be evaluated for in vivo usage for treatment of urinary tract infections. Manuscript profile
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  10 - Isolation and Characterization of the Symbiotic-Pathogenic Bacteria isolated from Trifolium Resupinatum Plant from the Southern Region of Isfahan, Iran
  Nazanin Moazenpour Monir Doudi Ladan Rahimzadeh Torabi Mohammad Hossein Pazandeh
  10.30495/bioem.2023.706514
  Plant diseases have a significant impact on plants and their crop yields, causing extensive epidemics and recurrent damages that result in profound negative effects. Bacteria such as Erwinia, Pectobacterium, and Klebsiella have a very wide host range and can play a path More

  Plant diseases have a significant impact on plants and their crop yields, causing extensive epidemics and recurrent damages that result in profound negative effects. Bacteria such as Erwinia, Pectobacterium, and Klebsiella have a very wide host range and can play a pathogenic role for a large number of ornamental and agricultural plants or even establish a symbiotic relationship with the plant. This group of bacteria that cause all kinds of plant diseases are able to affect seed tubers and the soil microbial community. The objective of this investigation was to identify and classify the distinct symbiotic pathogens associated with Trifolium resupinatum plants obtained from the Shahreza region located in the southern part of Isfahan, Iran. In this investigation, T. resupinatum specimens harboring nodular root structures were initially identified and subsequently retrieved from various locations in the southern region of Isfahan (Shahreza), before being transported to the laboratory. The Yeast Mannitol Agar (YMA) medium underwent a cultivation procedure, subsequent to which the bacterial samples were subjected to molecular identification utilizing morphological and biochemical tests. Additionally, the colony-PCR technique was employed to achieve definitive identification. This study examined the molecular features of three distinct species namely Erwinia chrysanthemi, Pectobacterium carotorum, and Klebsiella oxytoca. It was revealed that the former two species exhibited a symbiotic pathogenic relationship with the T. resupinatum plant, while the latter species posed a threat to human health as a pathogen. Manuscript profile
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  11 - Antimicrobial effects of Chitosan films incorporating with Lavandula stoechas on some food-borne bacteria
  Zohreh Mashak سمیرا فیاض فر نرجس چراغی
  Today, application of natural antimicrobial films as an inhibitors of growth of dangerous microbes especially in food staffs have been increased. The present study was carried out in order to study the antimicrobial activity of chitosan films containing 1–4% (v &f More

  Today, application of natural antimicrobial films as an inhibitors of growth of dangerous microbes especially in food staffs have been increased. The present study was carried out in order to study the antimicrobial activity of chitosan films containing 1–4% (v ⁄ v) Lavandula stoechas essential oil (EO) on some food-borne pathogenic bacteria (Listeria monocytogenes, Bacillus cereus, Vibrio parahaemolyticus, Staphylococcus aureus and Escherichia coli O157:H7). At first, the essential oil of the Lavandula stoechas herb was prepared. The most essential components are 1,8 cineol (16/49%), borneol (75/10%), camphor (94/8%) and linalool L (95/3%) and alfa-pinene (2/3 %).Then Circular discs of chitosan films containing different concentration of Lavandula stoechas essential oil were placed on bacterial lawn. Chitosan films incorporated with 4% (v ⁄ v) of EO inhibited the growth of all tested pathogenic bacteria. Incorporation of Chitosan film with EO showed significant antimicrobial effects (P < 0.05). Transparency of film was reduced by increasing of EO. These results showed that Chitosan films containing Lavandula stoechas EO have good effect for packaging some foods. Manuscript profile
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  12 - The Survey of anticancer and anti Microbial properties of Rosmarinus officinalis L, Peganum harmala L and Coffee Extracts on cell viability of Cancer cells and Pathogenic Bacteria in Foods
  Ali Sharifzadeh faranak aali sayeh vahhabi
  Despite significant advances in radiation therapy and cancer treatments in the past 30 years, resistance to chemotherapy is a major obstacle in the recovery of patients with cancer. Resistance to chemotherapy drugs inhibits the recovery process. This study aims to evalu More

  Despite significant advances in radiation therapy and cancer treatments in the past 30 years, resistance to chemotherapy is a major obstacle in the recovery of patients with cancer. Resistance to chemotherapy drugs inhibits the recovery process. This study aims to evaluate the anticancer activity of the hydroalcolic extracts of Rosemary, Peganum and Coffee on growth inhibition of MCF-7 cell line and determine the rate of growth inhibition of pathogenic bacteria isolated from food.We examined cytotoxicity effects of three different concentrations (60,120,250 μg/ml) of Rosemary, Peganum and Coffee extracts on MCF-7and HDF cell lines. . The standard MTT assay was performed to estimate cell viability after treatment by Rosemary, Peganum and Coffee extracts. The extracts were also used with MIC on Staphylococcus aureus and E. coli bacteria isolated from food.The results of the MTT assay showed that the Rosemary, Peganum and Coffee extracts had time-and concentration-dependent anticancer activities on the MCF-7cell line compared to HDF cells statistically significant (p Manuscript profile
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  13 - Composition and antibacterial activity of the chemical essential oil of Foeniculum vulgare and Eucalyptus to control some important plant pathogenic bacteria
  mitra omidi nasab Milad Aeini
  One of the most significant current discussions is approaching the antibacterial activity of essential oil to control plant pathogenic agents. Current study aimed to characterize chemical composition and antibacterial activity of Foeniculum vulgare and Eucalyptus to con More

  One of the most significant current discussions is approaching the antibacterial activity of essential oil to control plant pathogenic agents. Current study aimed to characterize chemical composition and antibacterial activity of Foeniculum vulgare and Eucalyptus to control some plant pathogenic bacteria in-vitro. In the present study, essential oil components of Foeniculum vulgare and Eucalyptus were subjected to Gas chromatography–mass spectrometry analysis, their minimum inhibitory concentration and antibacterial inhibitory on some plant pathogenic bacteria were determined. GC-MS analysis showed that the main component of the essential oil of F. vulgare were p-cymene, (30.18%), Cuminal (24.74%), 2-beta pinene (11.81%), while Eucalyptus were 1,8 cineol (47.87%), 1-alpha pinene (17.94%), p-cymene (7.91%) and Limonene (7.68%). Also, minimum inhibitory concentration was 3.79 µg/ml and 8.54 µg/ml for F. vulgare and Eucalyptus, respectively. Results of the antimicrobial investigation demonstrated that F. vulgare had the highest inhibition diameter of 4.4 centimeters in Clavibacter michiganensis. In Eucalyptus essential oil, highest inhibition zone (4.3 centimeters) were attributed to Erwinia amylovora. Results showed that by increasing the concentration, antibacterial activity increases. Our findings provide a rational basis of a novel emerging alternative to antimicrobial treatments in plant disease management programs. Manuscript profile
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  14 - Assessment of the antioxidant and antibacterial effects of stem and leaf alcoholic extracts of Dendrostellera lesserti
  Mostafa Alamhulu Sonbol Nazeri
  Background & Objectives: The increases in the microbial antibiotic resistance and decreases in the sensitivity of bacteria to antimicrobial chemical compounds are some concerns of the researchers who look for herbal compounds as antimicrobial properties. The purpose More

  Background & Objectives: The increases in the microbial antibiotic resistance and decreases in the sensitivity of bacteria to antimicrobial chemical compounds are some concerns of the researchers who look for herbal compounds as antimicrobial properties. The purpose of this study is to investigate the antibacterial and antioxidant properties of leaf and stem extracts of Dendrostellera lesserti against 10 human pathogenic bacteria. Material & Methods: Dendrostellera lesserti was collected from the Alvand Mountain (1961 m height) located at Hamedan province. After identification, the extracts were prepared using maceration method. In this sectional study, antibacterial activity was determined by agar well diffusion method, MIC (serial dilution method) and MBC. The antioxidant properties of these extracts was determined by DPPH method. Also, the amounts of phenol and flavonoid was assayed by Folin-ciocalteu and Aluminum chloride methods, respectively. Results: The largest growth inhibition zone with 27.3±0.6 mm diameter was observed on Micrococcus luteus culture exposed to ethanolic extract of the stem. The MIC and MBC of the extracted obtained from stem were lower than the leaf extract. Methanolic extract of stem in concentration of 1 mg/ml had the highest scavenging percentage of free radical. The methanol extracts of stem and leaf achieved the highest amount of phenol and flavonoid, 79.4±0.5 (mgGAE/g) and 2.1±0.1(mgQ/g), respectively. Conclusion: The results of present study showed that alcoholic extract, especially methanol extract, of Dendrostellera lesserti showed antibacterial ability. Since the phenolic and flavonoids compounds have anti­bacterial and anti-oxidant properties, the property showed in this study can be because of the presence of these compounds in the tissues studied. Manuscript profile
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  15 - Isolation and identification of microflora of farm ponds and gastrointestinal tract of western white shrimp (Litopenaeus vannamei) beside their evaluation as probiotic
  Mina Ziarati Mehran Avakh Keysami Farshid Kafilzadeh
  Background and Objectives: Nowadays, Shrimp culture has been prevailed in most of tropical countries; however, a variety of diseases have currently affected the business worldwide. Infectious bacterial agents are the most significant hygienic problems observed in the cu More

  Background and Objectives: Nowadays, Shrimp culture has been prevailed in most of tropical countries; however, a variety of diseases have currently affected the business worldwide. Infectious bacterial agents are the most significant hygienic problems observed in the cultured shrimps . This study was aimed to isolate and identify microflora of farm ponds and western white shrimp gastrointestinal tract (Litopenaeus vannamei) in the Bushehr Province farm ponds and their survey as probiotic. Materials and Methods: The shrimp and pond water samples were collected from 10 farm ponds (located at Bushehr Province). After transferring the samples to laboratory, the shrimp samples were autopsied to access to their intestinal microflora. The isolated bacteria were identified based on biochemical tests, morphology and Biolog software. Antibacterial activities of the isolates against 6 common pathogenic bacteria in shrimps were then investigated using well diffusion and disk diffusion methods. Results: Overall, 12 bacterial genera were isolated from the farm ponds and shrimp gastrointestinal tract. The majority of bacterial species most abundantly isolated were Bacillus, Vibrio and Pseudomonas. Among the isolated bacteria, Bacillus, Micrococcus and Corynebacterium showed the most antibacterial activity against evaluated pathogenic bacteria. Conclusion: Almost all the isolated bacteria from shrimp gastrointestinal tract were found in farm ponds as well. The bacterium Bacillus sp., for instance, is able to inhibit the growth of pathogens through secreting antimicrobial products. Manuscript profile