Assessment of the antioxidant and antibacterial effects of stem and leaf alcoholic extracts of Dendrostellera lesserti

Subject Areas : Medical Microbiology

Mostafa Alamhulu ¹ , Sonbol Nazeri ²

1 - MS.c., Department of Biotechnology, Agricultural Faculty, Bu-Ali Sina University, Hamedan, Iran.
2 - Assistant Professor, Department of Biotechnology, Agricultural Faculty, Bu-Ali Sina University, Hamedan, Iran.

Received: 2014-08-29 Accepted : 2014-11-14 Published : 2015-01-01

Keywords: Antioxidant, Antibacterial, Dendrostellera lesserti, Human pathogenic bacteria,

Abstract :

Background & Objectives: The increases in the microbial antibiotic resistance and decreases in the sensitivity of bacteria to antimicrobial chemical compounds are some concerns of the researchers who look for herbal compounds as antimicrobial properties. The purpose of this study is to investigate the antibacterial and antioxidant properties of leaf and stem extracts of Dendrostellera lesserti against 10 human pathogenic bacteria. Material & Methods: Dendrostellera lesserti was collected from the Alvand Mountain (1961 m height) located at Hamedan province. After identification, the extracts were prepared using maceration method. In this sectional study, antibacterial activity was determined by agar well diffusion method, MIC (serial dilution method) and MBC. The antioxidant properties of these extracts was determined by DPPH method. Also, the amounts of phenol and flavonoid was assayed by Folin-ciocalteu and Aluminum chloride methods, respectively. Results: The largest growth inhibition zone with 27.3±0.6 mm diameter was observed on Micrococcus luteus culture exposed to ethanolic extract of the stem. The MIC and MBC of the extracted obtained from stem were lower than the leaf extract. Methanolic extract of stem in concentration of 1 mg/ml had the highest scavenging percentage of free radical. The methanol extracts of stem and leaf achieved the highest amount of phenol and flavonoid, 79.4±0.5 (mgGAE/g) and 2.1±0.1(mgQ/g), respectively. Conclusion: The results of present study showed that alcoholic extract, especially methanol extract, of Dendrostellera lesserti showed antibacterial ability. Since the phenolic and flavonoids compounds have anti­bacterial and anti-oxidant properties, the property showed in this study can be because of the presence of these compounds in the tissues studied.