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حرية الوصول المقاله
1 - Understanding the phytochemical constitution, antioxidant potential and spectral characteristics of aqueous extracts of the chosen leafy vegetables from south India
Sobha Kota Pradeep Dumpala Hariseetharam Prasad Dachepalli Anantha Ratna KumariThe phytochemical composition, total phenolic and ascorbic acid contents of the aqueous extracts of six south Indian leafy vegetables viz. Amaranthus viridis L, Hibiscus cannabinus L, Spinacea oleracea L, Mentha spicata L, Murraya koenigii L and Coriandrum sativum L wer أکثرThe phytochemical composition, total phenolic and ascorbic acid contents of the aqueous extracts of six south Indian leafy vegetables viz. Amaranthus viridis L, Hibiscus cannabinus L, Spinacea oleracea L, Mentha spicata L, Murraya koenigii L and Coriandrum sativum L were tested, characterized and the antioxidant potential evaluated. Also, aqueous leaf extracts of A. viridis and H. cannabinus were demonstrated for their ability to form silver nanoparticles. All the aqueous extracts demonstrated the presence of flavonoids, saponins, tannins (except M. koenigii) and terpenoids (except S. oleracea) while phlobatannins (only in M. spicata), steroids, cardiac glycosides, alkaloids and proteins were absent in all the leaves tested. Carbohydrates were found only in the aqueous extract of H. cannabinus. The total phenolic content was the order of 0.9, 0.2, 1.8, 0.8 and 2.1 mgGAE/g of the leaves for A. viridis, H. cannabinus, M. spicata, M. koenigii and C. sativum respectively while S. oleracea did not respond to total phenolic content assay by FC method. Ascorbic acid, present in all the leafy vegetables with the lowest value of 0.3 mg/g in M. spicata and highest value of 1.9 mg/g in C. sativum. A highly significant DPPH radical scavenging activity was found in H. cannabinus (82.76%) followed by M. spicata (78.08%) and C. sativum (69.76%) at an extract concentration of 31.25 mg/ml while A. viridis, S. oleracea and M. koenigii showed 72.95%, 58.27% and 75.38% respectively at a concentration of 50 mg/ml. FT-IR spectral characterization of the extracts and the synthesized silver nanoparticles (Ag NPs) indicated the presence of N-H amines, O-H stretch, C-H out of plane bending vibrations, C=C stretching of alkenes, C=O stretch of amide and C≡C stretch of alkynes. The leafy vegetables can be used for the synthesis of lead compounds which will cure diseases. تفاصيل المقالة -
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2 - Simultaneous optimization of extraction of bioactive compounds and antioxidant activity of aerial parts of Ammi visnaga L. using response surface methodology
Zineb El Jabboury Smail Aazza Driss Ousaaid Oumaima Chater Uros Gasic Peđa Janaćković Zora Dajic Stevanovic Stefan Kolašinac Meryem Benjelloun Lahsen El GhadraouiDifferent extracts of aerial parts of Ammi visnaga (flowers, leaves, and stems) were prepared using different solvents, including water, methanol, and ethanol. The design of mixtures was carried out using different extractor solvents and their combinations. The special أکثرDifferent extracts of aerial parts of Ammi visnaga (flowers, leaves, and stems) were prepared using different solvents, including water, methanol, and ethanol. The design of mixtures was carried out using different extractor solvents and their combinations. The special cubic model explained the variance of the TPC and the antioxidant activity of the extracts at a level of R2 greater than 95%. The analysis of the model-derived response surfaces revealed that in binary mixtures (50% ethanol and 50% methanol) the yielded values of phenolic compounds and the antioxidant activity increase with the water proportion of different mixtures. The water content significantly influenced the ability of the quaternary mixture to extract the phenolic compounds. The main phytochemicals found were chlorogenic acid, caffeic acid, rutin, p-coumaric acid, and so on, especially flower extract. The different parts of A.visnaga were considered as a dense source of bioactive compounds. تفاصيل المقالة -
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3 - Effect of solvent, time and method of extraction on the amounts of phenolic, flavonoid and the antioxidant activity of the extract of <i>Ixiolirion tataricum</i> (Pall.) Schult. & Schult.f.
Fatemeh Rabbani Malihe Samadi KazemiSolvent type, extraction time and method are among important parameters in the evaluation of extraction efficiency. The selection of appropriate conditions to increase the extraction efficiency is of great importance. The response surface method (RSM) is a statistical a أکثرSolvent type, extraction time and method are among important parameters in the evaluation of extraction efficiency. The selection of appropriate conditions to increase the extraction efficiency is of great importance. The response surface method (RSM) is a statistical and mathematical technique which can be used to examine the dependence between the responses with variables and also to determine the optimal conditions. In this study, the experimental design of general full factorial (GFF) was used using Minitab 17 software. The variables were time (60, 90 and 120 min), solvent (water, methanol and n-hexane) and method (soxhlet and ultrasound-assisted extraction). The main effects and also interaction terms were investigated on amounts of total phenolic content (TPC), total flavonoid content (TFC) and the antioxidant activity (according to IC50, FRAP and BCB) of the extract of Ixiolirion tataricum (Pall.) Schult. & Schult.f.. Under optimal conditions, the best extraction method for the TFC was the ultrasound-based approach, whereas for other responses the soxhlet-based was better. Water was the optimal solvent for TPC, FRAP and BCB, but methanol was found as the best solvent for the determination of TFC and IC50. Also, the extraction time for all of the responses was 90 min. The amounts of TPC, TFC, IC50, FRAP and BCB in the optimum conditions obtained were 22.45 ± 0.60 (mg GAE/g extract), 114.57 ± 1.59 (mg QC/g extract), 0.36 ± 0.08 (mg/mL), 1014.7 ± 12.4 (mmol Fe2+/g extract) and 53.02 ± 0.05, respectively. The R² values for responses were close to unity, which indicates the compatibility between the experimental and the real data. A linear correlation was observed between phenolic compounds and antioxidant activity. Also, the effect of solvent was more important than time and extraction method on the amounts of TPC, TFC, IC50, FRAP and BCB. تفاصيل المقالة -
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4 - تأثیر 6-بنزیلآمینوپورین و متیل جاسمونات در بهبود عملکرد و کیفیت توتفرنگی رقم آلبیون
فرزاد عسگری سپیده کلاته جاری بابک متشرع زاده مرضیه قنبری جهرمی وریا ویسانیبهمنظور بررسی تأثیر 6-بنزیلآمینوپورین (6-BAP) و متیل جاسمونات (MJ) بر ویژگیهای مختلف کمی و کیفی توتفرنگی رقم آلبیون، آزمایشی بهصورت فاکتوریل در قالب طرح بلوکهای کامل تصادفی با سه تکرار در گلخانهای واقع در شهرستان دماوند اجرا شد. فاکتور اول، 6-BAP در دو سطح (شاهد أکثربهمنظور بررسی تأثیر 6-بنزیلآمینوپورین (6-BAP) و متیل جاسمونات (MJ) بر ویژگیهای مختلف کمی و کیفی توتفرنگی رقم آلبیون، آزمایشی بهصورت فاکتوریل در قالب طرح بلوکهای کامل تصادفی با سه تکرار در گلخانهای واقع در شهرستان دماوند اجرا شد. فاکتور اول، 6-BAP در دو سطح (شاهد و 100 میلیگرمدر لیتر) و فاکتور دوم،MJ در سه سطح (شاهد، 100 و 200 میکرومولار) بود. بوتهها در مرحله رشد رویشی دو بار با 6-BAP و در مرحلة زایشی سه نوبت با MJ محلولپاشی شدند. بر اساس نتایج، کاربرد 6-BAP در مرحلة رویشی، باعث تولید بوتههایی با تعداد برگ بیشتر و بزرگتر و طوقههایی قطورتر شد. بالاترین تعداد و وزن میوه، شاخص سبزینگی برگ، شاخص طعم، آنتوسیانین کل و طول عمر میوه، مربوط به تیمار 100 میلیگرمدر لیتر 6-BAP در شرایط استفاده از 200 میکرومولار MJ بود. کاربرد سیتوکینین توأم با هر یک از غلظتهای MJ، باعث بهبود معنیدار عملکرد بوته و سفتی بافت میوه نسبت به شاهد شد. بنابراین، میتوان بهرهگیری پیشازبرداشت از این تنظیمکنندههای رشد گیاهی را برای افزایش میزان تولید، بهبود کیفیت میوهها و افزایش عمر پسازبرداشت این محصول آسیبپذیر پیشنهاد نمود. تفاصيل المقالة -
حرية الوصول المقاله
5 - Growth and Biochemical Properties of Green Basil (<i>Ocimum basilicum</i> L.) Affected by Foliar Application of Biostimulants
Mozhgan Alibakhshi Hossein Ali Asadi-GharnehBACKGROUND: Green basil is a nutritional and medicinal plant due to its valuable properties. Improving qualitative and quantitative traits of plants with appropriate methods in sustainable agricultural were emphases. Biostimulants derived from organic materials and stim أکثرBACKGROUND: Green basil is a nutritional and medicinal plant due to its valuable properties. Improving qualitative and quantitative traits of plants with appropriate methods in sustainable agricultural were emphases. Biostimulants derived from organic materials and stimulate natural process in plants and improve crop performance. OBJECTIVES: In this research the effects of some biostimulants (amino acids, humic acid and seaweed extract) on morpho-physiological properties of green basil were evaluated. METHODS: This research was carried out under greenhouse condition. The experiment was conducted based on Completely Randomized Design with ten treatments and three replications. The treatments included control (distilled water), seaweed extract (0.5, 1 and 2 gr.l-1), amino acid (1, 2 and 3 gr.l-1) and humic acid (1, 2 and 3 ml.l-1). RESULT: The results showed that application of different biostimulants positive effects on vegetative parameters and biochemical properties of green basil plants. The highest leaf numbers (21.67) was measured in amino acid application at 1 g.l-1 concentration. The effect of humic acid was more noticeable on increased plant height (37.00- 47.67 cm) in compare to other biostimulants. The plants that received biostimulants had higher plant fresh weight than control treatment. Seaweed extract and humic acids increased significantly carotenoid content of plants. The highest ascorbic acid content was measured in treated plants at levels of 2 and 3 gr.l-1 amino acid. Application of all biostimulants were used in this study, had positive effects on concentration of total phenolic content of basil plants. CONCLUSION: According to the results of the present study, it can be concluded that, application of biostimulants at proper concentration due to positive effect on quality and quantity of growth parameters and biochemical properties of green basil plants are recommended. تفاصيل المقالة -
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6 - Evaluation of phenolic, flavonoid content and antioxidant capacity of the leaves, flowers, seeds and essential oil of Lavandula officinalis in comparison with synthetic antioxidants
شاهین مردانی نژادBackground & Aim: Lavandula officinalis is one of great importance due to its aromatic compounds and medicinal uses but not many studies have been done on the antioxidant power of different plant organs.Experimental: Antioxidant ability of Lavender leaves, flowers, أکثرBackground & Aim: Lavandula officinalis is one of great importance due to its aromatic compounds and medicinal uses but not many studies have been done on the antioxidant power of different plant organs.Experimental: Antioxidant ability of Lavender leaves, flowers, seeds and essential oil based on inhibition of free radicals and nitric oxide, inhibition of linoleic acid peroxidation by ferric thiocyanate and inhibition of malondialdehyde by thiobarbituric acid in compare with synthetic antioxidants Butyl Hydroxy Toluene (BHT) and Butyl Hydroxy Anisole (BHA) were measured.Results: The phenolic and flavonoid content of the leaf was 96.49±6.35 (µg gallic acid per mg dry weight of the extract) and 39.97±3.36 (µg of catechins per mg dry weight of the extract) respectively, more than other samples. In the study of antioxidant power, plant leaf extract with 48.66±5.5 μg was able to inhibit 50% of DPPH radicals, which had a weaker ability than synthetic antioxidants. The leaf extract of the plant had a higher ability than the synthetic antioxidant BHA to inhibit nitric oxide radicals and its ability was as high as BHT. The ability to inhibit the linoleic acid peroxidation of leaf and flower extracts at the beginning of the functional test showed similar BHT and BHA, at the end, the ability of leaf extract was stronger than BHA and weaker than BHT. The inhibitory potential of malondialdehyde leaf extract (82.66±1.5%) was better than BHA and weaker than BHT. Pearson correlation coefficients between phenolic content and antioxidant capacity of samples were high. Examination of leaf essential oil using GC-MS technique showed the presence of phenolic compounds in the plant.Recommended applications/industries: Due to the dangers of synthetic antioxidants in the food industry, the results of this study could introduce another application of this plant in terms of strong antioxidant properties. تفاصيل المقالة -
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7 - Effects of Pre-germination Treatment on the Phytate and Phenolic Contents of Almond Nuts
Liang Lin Lin Freda Xin You Giam Xin Min Foo Nadia Marie Hui Lian Yeo Charlene Jia Ling Koh Nur Hatika Binte Sa’Aban Wai Mun LokeThis study examined if pre-germination altered the water content and water activity, contents of phytate, total phenolic, (±)-catechin, quercetin and total antioxidant capacity of almond (Prunus dulcis)kernel. Raw almond kernels were submerged for 15 hours in wat أکثرThis study examined if pre-germination altered the water content and water activity, contents of phytate, total phenolic, (±)-catechin, quercetin and total antioxidant capacity of almond (Prunus dulcis)kernel. Raw almond kernels were submerged for 15 hours in water, 0.02 mol dm-3 phosphate buffer solution (pH 5.0) and 0.02 mol dm-3 phosphate buffer solution (pH 7.0) at 25 and 40ºC, respectively. The content and activity of water in the kernels before and after the pre-germination treatments were measured by oven drying and dew point water analysis, respectively. The total phenolic and phytic acid contents of the kernels were quantified by using Folin-Ciocalteu and a published spectrophotometric assay, respectively. (±)-Catechin and quercetin contents in the almond kernels were determined using gas-chromatography mass spectrometry. The total antioxidant capacity of the kernels were measured by 2,2’-diphenyl-1- picrylhydrazyl assay. Treatment with water, PBS pH 5 and PBS pH 7 significantly increased the water, total phenolic, (±)-catechin contents and total antioxidant capacity of the almond kernels regardless of the treatment temperatures (25 or 40°C). The phytic acid and quercetin contents were significantly elevated after the three treatments at 40°C. The total phenolic, (±)-catechin, quercetin and phytate contents in the almond kernels contributed significantly to its antioxidant property. Our results suggested that the phytochemical compositions of the almond kernels changed during pre-germination. The temperature and pH of the medium exert differential influence on the phytochemical compositions of the pre-germinated almond kernels. تفاصيل المقالة -
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8 - Contents of Aerial Parts of Salvia leriifolia Benth
Zahra Hosseinpoor Mohsen Abadi Behnam Mahdavi Esmaeil Rezaei-SereshtIn the present study, we have reported the total phenolic content, total flavonoid content, antioxidant and antimicrobial activity of aerial parts of Salvia leriifolia extracts and fractions. Methanolic, n-hexane, chloroform, and ethyl acetate extracts were screened to أکثرIn the present study, we have reported the total phenolic content, total flavonoid content, antioxidant and antimicrobial activity of aerial parts of Salvia leriifolia extracts and fractions. Methanolic, n-hexane, chloroform, and ethyl acetate extracts were screened to analysis their antioxidant activities by four complementary test systems, namely DPPH free radical scavenging activity (RSA), total phenolic content (TPC), total flavonoid content (TFC), and ferrous ion cheating (FIC). In most cases the leaf extracts and ethyl acetate fraction had more activity. The methanolic extracts of leaf and flower showed considerable antimicrobial activity using disc diffusion method against Escherichia coli, Streptococcus pneumonia, Acinetobacter, Serratia, Pseudomonas aeruginosa. The extracts showed the highest activity against P. aeruginosa and K. pneumonia. تفاصيل المقالة -
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9 - Simultaneous optimization of extraction of bioactive compounds and antioxidant activity of Ammi visnaga (L.) Lam aerial parts using response surface methodology
Zineb El Jabboury Smail Aazza Driss Ousaaid Oumaima Chater Uroš Gašić Peđa Janaćković Zora Dajic Stevanovic Stefan Kolašinac Meryem Benjelloun Lahsen El GhadraouiIn this report, different extracts from the aerial parts of Ammi visnaga (L.), e.g., flowers, leaves, and stems were prepared using water, methanol, and ethanol. To optimize the extraction process, the design of mixtures was carried out using different extracting solven أکثرIn this report, different extracts from the aerial parts of Ammi visnaga (L.), e.g., flowers, leaves, and stems were prepared using water, methanol, and ethanol. To optimize the extraction process, the design of mixtures was carried out using different extracting solvents and their combinations. The special cubic model explained the variance of the TPC and the antioxidant activity of the extracts at a level of R2 > 95%. In general, the analysis of the model-derived response surfaces revealed that in binary mixtures (50% ethanol + 50% methanol), the yielded values of phenolic compounds and the antioxidant activity increase with the water proportion of different prepared mixtures. The ability of the quaternary mixture to extract the phenolic compounds was also positively and significantly influenced by the water content, creating a mild polar medium for the extraction of phenolic compounds. The phenolic profile of different extracts under study revealed the presence of a cocktail of active ingredients, including chlorogenic acid, caffeic acid, rutin, p-coumaric acid, etc. especially the flower extract of A. visnaga (L.). تفاصيل المقالة -
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10 - Biological activities of protocorms and stems extracts of Dendrobium transparens
Pusp Raj Joshi Bir Bahadur Thapa Krishna Chand Lasta Maharjan Mukti Ram Paudel Basant Pant Bijaya PantThis study assessed the phenolic and flavonoid contents, and antioxidant, antibacterial, as well as cytotoxic properties of the protocorm extract of Dendrobium transparens and compared it to its wild equivalents. Methanol was used to extract compounds from the stems (DT أکثرThis study assessed the phenolic and flavonoid contents, and antioxidant, antibacterial, as well as cytotoxic properties of the protocorm extract of Dendrobium transparens and compared it to its wild equivalents. Methanol was used to extract compounds from the stems (DTSE) and protocorms (DTPE). DTSE contained 61.889 mg QE and 82.00 mg GAE per gram of quercetin and gallic acid, respectively. At a concentration of 191.23 μg/mL, DTSE exhibited a 50% DPPH radical scavenging efficiency. Compared to the 3T3 cell line (2108.87 μg/mL), the DTPE's cytotoxic ability against the HeLa (229.30 μg/mL) and U251 (213.90 μg/mL) cell lines was found to be significantly stronger. However, the U251 cell line was strongly cytotoxic to DTSE (75.84 μg/mL). At a dose of 2000 mg/kg, neither DTSE nor DTPE caused any discernible harm in mice. They could inhibit the growth of Acinetobacter baumannii, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Staphylococcus aureus. Based upon the experimental results, the wild stems and protocorms were found to be alternatives suitable for creating pharmacologically bioactive substances. تفاصيل المقالة
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