Biological activities of protocorms and stems extracts of Dendrobium transparens

الموضوعات :

Pusp Raj Joshi ¹ , Bir Bahadur Thapa ² , Krishna Chand ³ , Lasta Maharjan ⁴ , Mukti Ram Paudel ⁵ , Basant Pant ⁶ , Bijaya Pant ⁷

1 - Central Department of Botany, Tribhuvan University, Kirtipur 44613, Kathmandu, Nepal
2 - Central Department of Botany, Tribhuvan University, Kirtipur 44613, Kathmandu, Nepal
3 - Annapurna Research Center, Tanka Prasad Ghumti Sadak 44600, Kathmandu, Nepal
4 - Annapurna Research Center, Tanka Prasad Ghumti Sadak 44600, Kathmandu, Nepal
5 - Central Department of Botany, Tribhuvan University, Kirtipur 44613, Kathmandu, Nepal
6 - Annapurna Research Center, Tanka Prasad Ghumti Sadak 44600, Kathmandu, Nepal
7 - Central Department of Botany, Tribhuvan University

تاريخ الإرسال : 30 الجمعة , صفر, 1445 تاريخ التأكيد : 10 الأحد , ذو الحجة, 1445 تاريخ الإصدار : 18 السبت , ربيع الأول, 1446

الکلمات المفتاحية: Antibacterial activity, Antioxidant activity, Dendrobium transparens, MTT, Protocorms, Total flavonoid content (TFC), Total phenolic content (TPC),

ملخص المقالة :

This study assessed the phenolic and flavonoid contents, and antioxidant, antibacterial, as well as cytotoxic properties of the protocorm extract of Dendrobium transparens and compared it to its wild equivalents. Methanol was used to extract compounds from the stems (DTSE) and protocorms (DTPE). DTSE contained 61.889 mg QE and 82.00 mg GAE per gram of quercetin and gallic acid, respectively. At a concentration of 191.23 μg/mL, DTSE exhibited a 50% DPPH radical scavenging efficiency. Compared to the 3T3 cell line (2108.87 μg/mL), the DTPE's cytotoxic ability against the HeLa (229.30 μg/mL) and U251 (213.90 μg/mL) cell lines was found to be significantly stronger. However, the U251 cell line was strongly cytotoxic to DTSE (75.84 μg/mL). At a dose of 2000 mg/kg, neither DTSE nor DTPE caused any discernible harm in mice. They could inhibit the growth of Acinetobacter baumannii, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Staphylococcus aureus. Based upon the experimental results, the wild stems and protocorms were found to be alternatives suitable for creating pharmacologically bioactive substances.

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