بررسی میزان آلودگی بروسلا در شیرهای خام گوسفندی و اثر پالیده عاری از سلول (CFS) لاکتوباسیلوس اسیدوفیلوس روی تولید بیوفیلم آن

محورهای موضوعی : بهداشت مواد غذایی

مسعود پرانداخته ¹ , مسلم نیریز نقدهی ²

1 - دانش‌آموخته دکتری عمومی دامپزشکی، واحد ارومیه، دانشگاه آزاد اسلامی، ارومیه، ایران
2 - استادیار گروه بهداشت مواد غذایی، واحد ارومیه، دانشگاه آزاد اسلامی، ارومیه، ایران

تاریخ دریافت : 1401/06/02 تاریخ پذیرش : 1401/07/25 تاریخ انتشار : 1401/06/01

کلید واژه: بیوفیلم, لاکتوباسیلوس اسیدوفیلوس, بروسلا, پالیده عاری از سلول (CFS), شیر‌خام گوسفند, مهاباد (ایران),

چکیده مقاله :

بروسلوز از بیماری‌های باکتریایی زئونوتیک مهم در انسان و حیوانات مهره‌دار است. باکتری‌های اسید‌لاکتیک و ترکیبات تولیدی آن‌ها به‌عنوان عوامل کنترل‌زیستی بالقوه در تشکیل بیوفیلم مطرح شده‌اند. تحقیق حاضر با هدف تعیین اثرات پالیده عاری از سلول (CFS) لاکتوباسیلوس اسیدوفیلوس روی تولید بیوفیلم جدایه‌های بروسلا از شیرهای خام گوسفندی شهرستان مهاباد انجام شد. در این تحقیق، 100 نمونه شیر گوسفندی به‌‌صورت تصادفی از مناطق مختلف شهری و روستایی شهرستان مهاباد جمع‌آوری شدند. برای جداسازی بروسلا، ابتدا نمونه‌ها در آبگوشت بروسلا و سپس در آگار بروسلا با مکمل انتخابی کشت داده شدند؛ و برای شناسایی گونه‌های بروسلا، آزمایش‌های متابولیکی مرتبط انجام گردید. قابلیت تولید بیوفیلم جدایه‌های بروسلا و نیز فعالیت ضد‌بیوفیلم CFS لاکتوباسیلوس اسیدوفیلوس به روش سنجش میکروپلیت ارزیابی شد. یافته‌ها نشان داد که از مجموع 100 نمونه شیر، تعداد 3 نمونه (3 درصد) آلوده به بروسلا ملی‌تنسیس بودند. هم‌چنین هر سه جدایه دارای توانایی تشکیل بیوفیلم تشخیص داده شدند. دو‌ جدایه، تولید کننده متوسط بیوفیلم و یک جدایه، مولد قوی بیوفیلم بودند. غلظت‌های مختلف CFS، به‌صورت معنی‌داری (01/0˂p) تولید بیوفیلم جدایه‌ها را کاهش دادند. هم‌چنین غلظت 10 درصد CSF، بیشترین فعالیت ضد‌بیوفیلم را نشان داد. از یافته‌ها می‌توان استنباط نمود که آلودگی بروسلا در شیرهای خام گوسفندی منطقه مهاباد نسبتاً بالاست. اجرای کامل برنامه‌های غربالگری و مایه‌کوبی بروسلوز در گله‌های دامی منطقه ضروری است. هم‌چنین می‌توان نتیجه‌گیری نمود که CFS لاکتوباسیلوس اسیدوفیلوس دارای قابلیت حذف بیوفیلم بروسلا می‌باشد. بنابراین، CFS لاکتوباسیلوس اسیدوفیلوس به‌عنوان یک ترکیب ضد بیوفیلم می‌تواند در کنترل رشد بروسلا مطرح گردد.

چکیده انگلیسی:

Brucellosis is one of the most important zoonotic bacterial diseases in humans and vertebrate animals. Lactic acid bacteria (LAB) and their production compounds have been proposed as potential biocontrol agents in biofilm formation. The present research aimed to determine the effects of cell-free supernatant (CFS) from Lactobacillus acidophilus on the biofilm production of Brucella isolates from raw sheep milk of Mahabad city (Iran). In this study, 100 sheep milk samples were randomly collected from different urban and rural areas of Mahabad. To isolate Brucella, the samples were cultured in Brucella broth and then streaked on Brucella agar with the selective supplement, and to identify the species of Brucella, related metabolic tests were performed. The biofilm production capability of the isolates, as well as the antibiofilm activity of the CFS, were evaluated by the microplate assay method. The results showed that out of 100 milk samples, 3 samples (3%) are contaminated with Brucella melitensis. Also, all three isolates were biofilm-formers. Two isolates were recognized as moderate and one isolate as a strong biofilm-former. Different concentrations of CFS reduced the biofilm production of isolates significantly (P˂ 0.01). Also, the concentration of 10% of CSF showed the highest antibiofilm activity. From the findings, it can be concluded that Brucella contamination in raw sheep milk in the Mahabad region is relatively high. There is a need for a full performance of Brucellosis screening and vaccination programs in livestock herds of the region. It can also be concluded that the CFS of L. acidophilus can remove Brucella biofilm. Therefore, L. acidophilus CFS as an antibiofilm compound can be proposed to control the growth of Brucella.

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