Physiological, Biochemical, and Developmental Responses of some Pistachio Genotypes under Drought Stress

Subject Areas : Abiotic stress physiology

Mostafa Ghasemi ¹ , Shiva Ghasemi ² , Mehdi Mohammadi-Moghadam ³ , Saeid Kashanizadeh ⁴ , Mansoore Shamili ⁵

1 - Horticulture Crops Research Department, Qazvin Agricultural and Natural Resources Research and Education Center, AREEO, Qazvin, Iran
2 - Horticulture Crops Research Department, Qazvin Agricultural and Natural Resources Research and Education Center, AREEO, Qazvin, Iran
3 - Horticulture Crops Research Department, Semnan Agricultural and Natural Resources Research and Education Center, AREEO, Shahrood, Iran
4 - Horticulture Crops Research Department, Qazvin Agricultural and Natural Resources Research and Education Center, AREEO, Qazvin, Iran
5 - Associate professor, Agriculture Faculty, Hormoz Research Center, University of Hormozgan, Bandar Abbas, Iran

Received: 2023-07-15 Accepted : 2023-10-11 Published : 2024-06-08

Keywords: Proline, Ion leakage, Vegetative growth, Leaf relative water content,

Abstract :

Pistachio is one of the economic nut fruits in Iran. Water limitation is the most restrictive factor for its production. To overcome the water scarcity crisis, introducing drought-tolerant rootstocks is among the crucial breeding strategies. To investigate the drought tolerance of five Qazvin native pistachio genotypes, an experiment was carried out as a factorial experiment in a completely randomized design with four replications in the greenhouse conditions. The factors were pistachio genotypes (Madari, KalKhandan, Kalehbozi, Sefid, and Ghermez) and irrigation regime (normal conditions and drought stress). The highest relative water content under drought irrigation conditions belonged to the Sefid (59.99%), and Ghermez (59.09%) genotypes. The lowest value (54.68%) belonged to the Madari genotype. The highest electrolyte leakage under drought irrigation conditions belonged to the Madari genotype (55.75%). The lowest electrolyte leakage (42.44%) belonged to the Sefid genotype. Under drought stress, the highest amount of chlorophyll a (2.12 mg g^-1 fresh weight), total chlorophyll (3.051 mg g^-1 fresh weight), and carotenoid (2.38 mg g^-1 fresh weight) was observed in Ghermez genotype. In contrast, the highest amount of chlorophyll b (1.34 mg g^-1 fresh weight) was observed in the Sefid genotype. The lowest amounts of chlorophyll and carotenoid in the drought stress belonged to the Madari genotype. According to the results, the Ghermez and Sefid genotypes with lower electrolyte leakage and higher relative water content, chlorophyll, carotenoid, and biomass under water stress, were the more drought-tolerant genotypes. Madari and KalKhandan genotypes with higher electrolyte leakage and lower relative leaf water content, chlorophyll, and biomass were the most drought-sensitive genotypes.

References:

Anonymous (2019) Annual agricultural statistics. Ministry of Jihad-e-Agriculture of Iran. Available from. http://www.maj.ir.

Arab MM, Marrano A, Abdollahi-Arpanahi R, Leslie CA, Cheng H, Neale DB, Vahdati K. (2020). Combining phenotype, genotype, and environment to uncover genetic components underlying water use efficiency in Persian walnut. Journal of Experimental Botany 23; 71(3), 1107-1127.

Arnon AN (1967) Method of extraction of chlorophyll in the plants. Agronomy Journal. 23, 112-121.

Asayesh ZM, Vahdati K, Aliniaeifard S (2017) Investigation of physiological components involved in low water conservation capacity of in vitro walnut plants. Scientia Horticulturae. 224, 1-7.

Atkinson CJ, Else MA, Taylor L, Dover CJ (2003) Root and stem hydraulic conductivity as determinants of growth potential in grafted trees of apple (Malus pumila Mill.). Journal of Experimental Botany. 54, 1221-1229

Balaguer L, Pugnaire FI, Martinez-Ferri E, Armas C, Valladares F, Manrique E (2002) Ecophysiological significance of chlorophyll loss and reduced photochemical efficiency under extreme aridity in Stipa tenacissima L. Plant and Soil. 240, 343-352.

Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water-stress studies. Plant and Soil. 39, 205-207.

Behzadi Rad P, Roozban MR, Karimi S, Ghahremani R, Vahdati K (2021) Osmolyte accumulation and sodium compartmentation has a key role in salinity tolerance of pistachios rootstocks. Agriculture. 11(8), 708.

Calatayud A, Roca D, Martínez PF (2006) Spatial-temporal variations in rose leaves under water stress conditions studied by chlorophyll fluorescence imaging. Plant Physiology and Biochemistry. 44, 564-573.

Dierks-Ventling C, Tonelli C (1982) Metabolism of Proline, Glutamate, and Ornithine in Proline Mutant Root Tips of Zea mays (L.). Plant Physiology. 69(1), 130–134.

Ebrahimi A and Vahdati K (2007) Improved success of Persian walnut grafting under environmentally controlled conditions. International Journal of Fruit Science. 6(4), 3-12.

Esmaeilpour A, Van Labekea MC, Samsonc R, Boeckxa P, Van Dammea P (2016) Variation in biochemical characteristics, water status, stomatal features, leaf carbon isotope composition and its relationship to water use efficiency in pistachio (Pistacia vera L.) cultivars under drought stress condition. Scientia Horticulturae. 211, 158-166.

Ghasemi M, Hasheminasab H, Ghasemi S, Kashanizadeh S (2021) Evaluation of Drought Tolerance of Some Native Pistachio Genotypes of Qazvin Province. Pistachio science and technology. 6(11), 102-122. [In Persian]

Gijon MC, Gimenez C, Gurrero DPL, Couceiro JF, Moriana A (2010) Rootstock influences the response of pistachio (Pistacia vera L. cv. Kerman) to water stress and rehydration. Scientia Horticulturae. 125(4), 666-671.

Hall RC, Twidwell EK (2002) Effects of Drought Stress on Soybean Production. Extension Extra. pp. 254.

Hoseini SS, Cheniany M, Lahouti M, Ganjeali A (2016) Evaluation of resistance to drought stress in seedlings of two lines of Triticale (Triticosecale × Wittmack) with emphasis on some enzymatic and non-enzymatic antioxidants. Iranian Journal of Plant Biology. 8(30), 3-42. [In Persian]

Irigoyen JH, Emerich DW, Sanchez Diaz M (1992) Water stress induced changes in concentration of proline and total soluble sugars in nodulated alfalfa (Medicago sativa) plants. Physiologia Plantarum. 84(1), 55-66.

Lotfi N, Soleimani A, Vahdati K, Çakmakçı R. (2019) Comprehensive biochemical insights into the seed germination of walnut under drought stress. Scientia Horticulturae 250, 329-43.

Lotfi N, Vahdati K, Kholdebarin B and Amiri R (2010) Drought-induced accumulation of sugars and proline in radicle and plumule of tolerant walnut varieties during germination phase. Acta Horticulturae. 861, 289-296.

Lovisolo C, Perrone I, Hartung W, Schubert A (2008) An abscisic acid-related reduced transpiration promotes gradual embolism repair when grapevines are rehydrated after drought. New Phytologist. 180(3), 642-651.

Mandhanis S, Madan S, Whney V (2006) Antioxidant defense mechanism under salt stress in wheat seedling. Journal of Biologia Plantarum. 50(2), 227-231.

Monreal JA, Jimenez ET, Remesal E, Morillo-Velarde R, Garcia-Maurino S, Echevarria C (2007) Proline content of sugar beet storage roots: Response to water deficit and nitrogen fertilization at field conditions. Environmental and Experimental Botany. 60(2), 257-267.

Morsy MR, Jouve L, Hausman JF, Hoffmann L, Stewart JMCD (2007) Alteration of oxidative and carbohydrate metabolism under abiotic stress in two rice (Oryza sativa L.) genotypes contrasting in chilling tolerance. Journal of Plant Physiology. 164(2), 157-167.

Nazoori F, ZamaniBahramabadi E, Mirdehghan H, Yousefi M (2022) Preharvest application of sulfur as pesticide on fresh hull and kernel of pistachio (Pistacia vera L.). International Journal of Horticultural Science and Technology. 9(1), 117-129.

Qinghua S, Zhiyi B, Zhujun Z, Quansheng Y, Qiong Q (2006) Effect of different treatment of salicylic acid on heat tolerance chlorophyll fluorescence, and antioxidant enzyme activity in seedling of Cucumis sativa L. Plant Growth Regulation. 48(2), 127-135.

Ritchie SW, Nguyen HT, Halody AS (1990) Leaf water content and gas exchange parameters of two wheat genotypes differing in drought resistance. Crop Sciences. 30(1), 105-111.

Sajjadinia A, Ershadi A, Hokmabadi H, Khayyat M, Gholami M (2010) Gas exchange activities and relative water content at different fruit growth and developmental stages of on and off cultivated pistachio trees. Australian Journal of Agricultural Engineering. 1(1), 1-6.

Schutz H, Fangmier E (2001) Growth and yield responses of spring wheat (Triticum aestivum L. cv. Minaret) to elevated CO₂ and water limitation. Environmental Pollution. 114(2), 187-194.

Shamshir MH, Hasani MR (2015) Synergistic accumulative effects between exogenous salicylic acid and arbuscular mycorrhizal fungus in pistachio (Pistacia Vera cv. Abareqi) seedlings under drought stress. International Journal of Horticultural Science and Technology. 2(2), 151-160

Sharifkhah M, Bakhshi D, Pourghayoumi M, Abdi S, Hokmabadi H (2020) Effect of pollination time on yield and antioxidant properties of some pistachio cultivars. International Journal of Horticultural Science and Technology. 7(1), 51-58.

Stuart L, Emel D, Bala AA, Hikmet B (2011) The drought response displayed by a DRE-binding protein from Triticum dicoccoides. Plant Physiology and Biochemistry. 49, 346-351.

Thapa R, Thapa P, Ahamad K, Vahdati K (2021) Effect of grafting methods and dates on the graft take rate of Persian walnut in open field condition. International Journal of Horticultural Science and Technology. 8(2), 133-47.

Tombesi S, Johnson RS, Day KR, DeJong TM (2010) Relationships between xylem vessel characteristics, calculated axial hydraulic conductance and size-controlling capacity of peach rootstocks. Annals of Botany. 105(2), 327-331.

Vahdati K, Sarikhani S, Arab MM, Leslie CA, Dandekar AM, Aletà N, Bielsa B, Gradziel TM, Montesinos Á, Rubio-Cabetas MJ, Sideli GM, Serdar Ü, Akyüz B, Beccaro GL, Donno D, Rovira M, Ferguson L, Akbari M, Sheikhi A, Sestras AF, Kafkas S, Paizila A, Roozban MR, Kaur A, Panta S, Zhang L, Sestras RE, Mehlenbacher S (2021) Advances in rootstock breeding of nut trees: objectives and strategies. Plants. 10, (11), 2234.

Whitlow TH, Bassuk NL, Ranney TG, Reichert LD (1992) An improved method for using electrolyte leakage to assess membrane competence in plant tissues. Plant Physiology. 98, 198-205.

Zoric L, Ljubojevic M, Merkulov LJ, Lukovic J, Ognjanov V (2012) Anatomical characteristics of cherry rootstocks as possible preselecting tools for prediction of tree vigor. Journal of Plant Growth Regulation. 3(31), 320-331.