مقایسه تأثیر حلال‌های هیدرواتانولی و آبی روی پتانسیل عملکردی و ترکیبات فعال عصاره کدوحلوائی (Cucurbita moschata)
الموضوعات :
زهرا لطیفی
1
(دانشجوی دکتری علوم و صنایع غذایی، دانشکده علوم و صنایع غذایی، دانشگاه آزاد اسلامی، نور، ایران)
سعید عابدیان کناری
2
(استاد ایمنی‌شناسی پزشکی، دانشکده پزشکی، مرکز تحقیقات ژنتیک ایمنی، دانشگاه علوم پزشکی مازندران، ساری، ایران)
علی اکبر مشایخ
3
(استادیار، گروه تربیت بدنی و علوم ورزشی، دانشکده علوم انسانی و اجتماعی، دانشگاه آزاد اسلامی، نور، ایران)
تاريخ الإرسال : 22 الخميس , شوال, 1442
تاريخ التأكيد : 27 الإثنين , صفر, 1443
تاريخ الإصدار : 12 الثلاثاء , ذو القعدة, 1442
الکلمات المفتاحية:
عصاره هیدرواتانولی,
عصاره آبی,
ترکیبات زیست فعال,
کدوحلوائی,
ملخص المقالة :
کدوحلوائی (Cucurbita moschata) یکی از سبزیجاتی است که دارای ارزش تغذیه ای بالا و ترکیبات زیست فعال است. هدف از این مطالعه، مقایسه تأثیر حلال های هیدرواتانولی و آبی روی پتانسیل عملکردی و بررسی ترکیبات زیست فعال عصاره کدوحلوائی می باشد. جهت بررسی های عملکردی عصاره ها، میزان آسکوربیک اسید، فنل و فلانوئید کل، ظرفیت آنتی اکسیدانی به دو روش DPPH و FRAP انجام گردید و همچنین ترکیبات شیمیایی موجود در عصاره ها توسط GC/MS شناسایی شدند. نتایج به‌دست‌آمده بالاترین میزان آسکوربیک اسید، فنل و فلاونوئید کل در عصاره هیدرواتانولی به ترتیب، با میزان mg/100g 29/13±66/98، mg GA/100g 004/0±663/1 و mg QU/100g 01/0±381/0 نشان داد. عصاره هیدرواتانولی نسبت به عصاره آبی در تمامی غلظت های تهیه‌شده، بالاترین توانائی مهار رادیکال DPPH را نشان داد؛ به‌طوری‌که عصاره هیدرواتانولی با غلظت mg/ml 800 با 866/61 درصد بازدارندگی، دارای بالاترین ویژگی مهارکنندگی بود. ترکیبات شیمیایی شناسایی‌شده در عصاره هیدرواتانولی و آبی با استفاده از GC/MS به ترتیب 16 و 7 ترکیب شیمیایی شناسایی شد که بالاترین میزان ترکیب در عصاره هیدرواتانولی برابر با 8767/34 درصد مربوط به ترکیب D- اریترو-پنتوز،2-دِاُکسی و در عصاره آبی مربوط به 5،2-دی کلرو فنل (2053/74 درصد) بود. نتایج این مطالعه، استفاده از حلال هیدرواتانولی را جهت استخراج بالاتر ترکیبات زیست فعال از کدوحلوائی (C. moschata) را پیشنهاد می دهد.
المصادر:
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Akpuaka, A., Ekwenchi, M., Dashak, D., and Dildar, A. (2012). Gas Chromatography-Mass Spectrometry (GC/MS) analysis of phthalate isolates in n-hexane extract of Azadirachta indica A. Juss (Neem) leaves. Journal of American Science, 8(12), 146-155.
Al-Qaisy, M. R., and Rathi, M. H. 2019. Total Phenolic Content and Antioxidant Efficacy of Three parts of the pumpkin Cucurbita moschata and the effect of the drying method on them. World Journal of Pharmacy and Pharmaceutical Sciences. 8(4): 1679-1689.
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Albishi, T., John, J. A., Al-Khalifa, A. S., and Shahidi, F. (2013). Phenolic content and antioxidant activities of selected potato varieties and their processing by-products. Journal of Functional Foods, 5(2), 590-600.
Alothman, M., Bhat, R., and Karim, A. (2009). Antioxidant capacity and phenolic content of selected tropical fruits from Malaysia, extracted with different solvents. Food chemistry, 115(3), 785-788.
Ani, V., Varadaraj, M., and Naidu, K. A. (2006). Antioxidant and antibacterial activities of polyphenolic compounds from bitter cumin (Cuminum nigrum). European Food Research and Technology, 224(1), 109-115.
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Bhandary, S. K., Kumari, S., Bhat, V. S., Sharmila, K., and Bekal, M. P. (2012). Preliminary phytochemical screening of various extracts of Punica granatum peel, whole fruit and seeds. J Health Sci, 2(4), 35-38.
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Chauhan, H., and Singh, M. 2019. Phytochemical characterization and antibacterial potential of Indian and Chinese cabbage genotypes against human pathogens in Uttarakhand, India. International Journal of Recent Scientific Research, 10(12): 36462-36466.
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Dhiman, A. K., Sharma, K., and Attri, S. (2009). Functional constitutents and processing of pumpkin: A review. Journal of Food Science and Technology, 46(5), 411.
Elfalleh, W., Kirkan, B., and Sarikurkcu, C. (2019). Antioxidant potential and phenolic composition of extracts from Stachys tmolea: An endemic plant from Turkey. Industrial Crops and Products, 127, 212-216.
Ellong, E. N., Billard, C., Adenet, S., and Rochefort, K. (2015). Polyphenols, carotenoids, vitamin C content in tropical fruits and vegetables and impact of processing methods. Food and Nutrition Sciences, 6(03), 299.
Enneb, S., Drine, S., Bagues, M., Triki, T., Boussora, F., Guasmi, F., Ferchichi, A. (2020). Phytochemical profiles and nutritional composition of squash (Cucurbita moschata) from Tunisia. South African Journal of Botany, 130, 165-171.
Hayouni, E. A., Abedrabba, M., Bouix, M., and Hamdi, M. (2007). The effects of solvents and extraction method on the phenolic contents and biological activities in vitro of Tunisian Quercus coccifera L. and Juniperus phoenicea L. fruit extracts. Food chemistry, 105(3), 1126-1134.
Indrianingsih, A., Rosyida, V., Apriyana, W., Hayati, S. N., Nisa, K., Darsih, C., Indirayati, N. (2019). Comparisons of antioxidant activities of two varieties of pumpkin (Cucurbita moschata and Cucurbita maxima) extracts. Paper presented at the IOP Conference Series: Earth and Environmental Science.
Jacobo-Valenzuela N, de Jesus Zazueta-Morales J, Gallegos-Infante JA, Aguilar-Gutierrez F, Camacho-Hernandez IL, Rocha-Guzman NE, et al. (2011). Chemical and physicochemical characterization of winter squash (Cucurbita moschata). Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 39(1), 34-40.
Jayasundara, C., Deraniyagala, S. A., Hettiarachchi, C., and Thiripuranathar, G. (2018). Antioxidant and antibacterial activities of leaves, skin, flesh and seeds of Sri Lankan variety of cucurbita moschata. International Journal of Ayurveda and Pharma Research.
Jung, C.-H., Seog, H.-M., Choi, I.-W., Park, M.-W., and Cho, H.-Y. (2006). Antioxidant properties of various solvent extracts from wild ginseng leaves. LWT-Food Science and Technology, 39(3), 266-274.
Kartal, N., Sokmen, M., Tepe, B., Daferera, D., Polissiou, M., and Sokmen, A. (2007). Investigation of the antioxidant properties of Ferula orientalis L. using a suitable extraction procedure. Food chemistry, 100(2), 584-589.
Kaur, G. (2018). Development of Functionalprobiotic Beverage from Pumpkin. Lovely Professional University,
Kim, M. Y., Kim, E. J., Kim, Y.-N., Choi, C., and Lee, B.-H. (2012). Comparison of the chemical compositions and nutritive values of various pumpkin (Cucurbitaceae) species and parts. Nutrition research and practice, 6(1), 21-27.
Kim, S.-J., Cho, A. R., and Han, J. (2013). Antioxidant and antimicrobial activities of leafy green vegetable extracts and their applications to meat product preservation. Food control, 29(1), 112-120.
Krishnaiah, D., Sarbatly, R., and Nithyanandam, R. (2011). A review of the antioxidant potential of medicinal plant species. Food and bioproducts processing, 89(3), 217-233.
Kulczyński, B., and Gramza-Michałowska, A. (2016). Goji berry (Lycium barbarum): composition and health effects–a review. Polish Journal of Food and Nutrition Sciences, 66(2), 67-76.
Kulczyński, B., and Gramza-Michałowska, A. (2019a). The profile of carotenoids and other bioactive molecules in various pumpkin fruits (Cucurbita maxima Duchesne) cultivars. Molecules, 24(18), 3212.
Kulczyński, B., and Gramza-Michałowska, A. (2019b). The profile of secondary metabolites and other bioactive compounds in Cucurbita pepo L. and Cucurbita moschata pumpkin cultivars. Molecules, 24(16), 2945.
Kulczyński, B., Gramza-Michałowska, A., and Królczyk, J. B. (2020). Optimization of Extraction Conditions for the Antioxidant Potential of Different Pumpkin Varieties (Cucurbita maxima). Sustainability, 12(4), 1305.
Mala, K. S., and Kurian, A. E. (2016). Nutritional composition and antioxidant activity of pumpkin wastes. International Journal of Pharmaceutical, Chemical & Biological Sciences, 6(3).
Montesano, D., Blasi, F., Simonetti, M. S., Santini, A., and Cossignani, L. (2018). Chemical and nutritional characterization of seed oil from Cucurbita maxima(var. Berrettina) pumpkin. Foods, 7(3), 30.
Okwu, D. E., and Ohenhen, O. (2010). Isolation and characterization of Steroidal Glycosides from the leaves of Stachytarpheta Jamaicensis Linn Vahl. Der Chemica Sinica, 1(2), 6-14.
Ordonez, A., Gomez, J., and Vattuone, M. (2006). Antioxidant activities of Sechium edule (Jacq.) Swartz extracts. Food chemistry, 97(3), 452-458.
Pandey, S., Singh, J., Upadhyay, A., Ram, D., and Rai, M. (2003). Ascorbate and carotenoid content in an Indian collection of pumpkin (Cucurbita moschata ex Poir.). Cucurbit Genetics Cooperative Report, 26, 51-53.
Ramroudi, M., Kazemitabar, S. K., Esmaeilzadeh Kenari, R., and Najafi Zarini, H. (2020). Evaluation of Antioxidant Activity of Hydroalcoholic Extracts from Pumpkin (Cucurbita moschata) Seed, Flesh and Skin. Iranian Journal of Biosystems Engineering, 51(3), 663-672.
Rane, Z., Anish-Kumar, P., and Bhaskar, A. (2012). Phytochemical evaluation by GC-MS and in vitro antioxidant activity of Punica granatum fruit rind extract. Journal of Chemical and Pharmaceutical Research, 4(6), 2869-2873.
Rathi, M., and Abdulhay, H. (2018). Total phenolic contents and antioxidant activity of extracts of Tea (Black, Green and White). Asian Jr. of Microbiol. Biotech. Env. Sc, 20(2018), 45-49.
Rezig, L., Chouaibi, M., Meddeb, W., Msaada, K., and Hamdi, S. (2019). Chemical composition and bioactive compounds of Cucurbitaceae seeds: Potential sources for new trends of plant oils. Process Safety and Environmental Protection, 127, 73-81.
Sauer, M., Porro, D., Mattanovich, D., and Branduardi, P. (2008). Microbial production of organic acids: expanding the markets. Trends in biotechnology, 26(2), 100-108.
Singh, J., Singh, V., Shukla, S., and Rai, A. (2016). Phenolic content and antioxidant capacity of selected cucurbit fruits extracted with different solvents. J Nutr Food Sci, 6(6), 1-8.
Singh, S., Singh, R., Thakur, P., and Kumar, R. (2018). Phytochemicals, functionality and breeding for enrichment of cole vegetables (Brassica oleracea). Phytochemicals in vegetables: a valuable source of bioactive compounds. Bentham Science Publishers, UAE, 256-295.
Stevenson, D. G., Eller, F. J., Wang, L., Jane, J.-L., Wang, T., and Inglett, G. E. (2007). Oil and tocopherol content and composition of pumpkin seed oil in 12 cultivars. Journal of agricultural and food chemistry, 55(10), 4005-4013.
Vale, A., Santos, J., Melia, N., Peixoto, V., Brito, N., and Oliveira, M. B. P. (2015). Phytochemical composition and antimicrobial properties of four varieties of Brassica oleracea sprouts. Food Control, 55, 248-256.
Wang, X., Wang, C., Zha, X., Mei, Y., Xia, J., and Jiao, Z. (2017). Supercritical carbon dioxide extraction of β-carotene and α-tocopherol from pumpkin: a Box–Behnken design for extraction variables. Analytical Methods, 9(2), 294-303.
Wu, H., Zhu, J., Diao, W., and Wang, C. (2014). Ultrasound-assisted enzymatic extraction and antioxidant activity of polysaccharides from pumpkin (Cucurbita moschata). Carbohydrate polymers, 113, 314-324.
Xie, X., Deng, T., Duan, J., Ding, S., Yuan, J., and Chen, M. (2019). Comparing the effects of diethylhexyl phthalate and dibutyl phthalate exposure on hypertension in mice. Ecotoxicology and environmental safety, 174, 75-82.
Yakoub, A. R. B., Abdehedi, O., Jridi, M., Elfalleh, W., Nasri, M., and Ferchichi, A. (2018). Flavonoids, phenols, antioxidant, and antimicrobial activities in various extracts from Tossa jute leave (Corchorus olitorus). Industrial Crops and Products, 118, 206-213.
Zaccari, F., and Galietta, G. (2015). α-Carotene and β-carotene content in raw and cooked pulp of three mature stage winter squash “type butternut”. Foods, 4(3), 477-486.
Zinash, A., and Woldetsadik, K. (2013). Effect of accessions on the chemical quality of fresh pumpkin. African Journal of Biotechnology, 12(51), 7092-7098.
_||_
Ahmad, G., and Khan, A. A. (2019). Pumpkin: horticultural importance and its roles in various forms; a review. Int J Hortic Agric, 4(1), 1-6.
Akpuaka, A., Ekwenchi, M., Dashak, D., and Dildar, A. (2012). Gas Chromatography-Mass Spectrometry (GC/MS) analysis of phthalate isolates in n-hexane extract of Azadirachta indica A. Juss (Neem) leaves. Journal of American Science, 8(12), 146-155.
Al-Qaisy, M. R., and Rathi, M. H. 2019. Total Phenolic Content and Antioxidant Efficacy of Three parts of the pumpkin Cucurbita moschata and the effect of the drying method on them. World Journal of Pharmacy and Pharmaceutical Sciences. 8(4): 1679-1689.
Al-Rikabi, A. K. J. (2007). Extraction of phenolic compounds from wheat bran and its assessment as antioxidants. Journal of Basrah Researches (Sciences), 33(2B).
Albishi, T., John, J. A., Al-Khalifa, A. S., and Shahidi, F. (2013). Phenolic content and antioxidant activities of selected potato varieties and their processing by-products. Journal of Functional Foods, 5(2), 590-600.
Alothman, M., Bhat, R., and Karim, A. (2009). Antioxidant capacity and phenolic content of selected tropical fruits from Malaysia, extracted with different solvents. Food chemistry, 115(3), 785-788.
Ani, V., Varadaraj, M., and Naidu, K. A. (2006). Antioxidant and antibacterial activities of polyphenolic compounds from bitter cumin (Cuminum nigrum). European Food Research and Technology, 224(1), 109-115.
Badr, S. E., Shaaban, M., Elkholy, Y. M., Helal, M. H., Hamza, A. S., Masoud, M. S., and El Safty, M. M. (2011). Chemical composition and biological activity of ripe pumpkin fruits (Cucurbita pepo) cultivated in Egyptian habitats. Natural product research, 25(16), 1524-1539.
Bhandary, S. K., Kumari, S., Bhat, V. S., Sharmila, K., and Bekal, M. P. (2012). Preliminary phytochemical screening of various extracts of Punica granatum peel, whole fruit and seeds. J Health Sci, 2(4), 35-38.
Biesalski, H.-K., Dragsted, L. O., Elmadfa, I., Grossklaus, R., Müller, M., Schrenk, D., Weber, P. (2009). Bioactive compounds: Definition and assessment of activity. Nutrition, 25(11-12), 1202-1205.
Chauhan, H., and Singh, M. 2019. Phytochemical characterization and antibacterial potential of Indian and Chinese cabbage genotypes against human pathogens in Uttarakhand, India. International Journal of Recent Scientific Research, 10(12): 36462-36466.
Chigayo, K., Mojapelo, P. E. L., Mnyakeni-Moleele, S., and Misihairabgwi, J. M. (2016). Phytochemical and antioxidant properties of different solvent extracts of Kirkia wilmsii tubers. Asian Pacific Journal of Tropical Biomedicine, 6(12), 1037-1043.
Dai, J., and Mumper, R. J. (2010). Plant phenolics: extraction, analysis and their antioxidant and anticancer properties. Molecules, 15(10), 7313-7352.
Dhiman, A. K., Sharma, K., and Attri, S. (2009). Functional constitutents and processing of pumpkin: A review. Journal of Food Science and Technology, 46(5), 411.
Elfalleh, W., Kirkan, B., and Sarikurkcu, C. (2019). Antioxidant potential and phenolic composition of extracts from Stachys tmolea: An endemic plant from Turkey. Industrial Crops and Products, 127, 212-216.
Ellong, E. N., Billard, C., Adenet, S., and Rochefort, K. (2015). Polyphenols, carotenoids, vitamin C content in tropical fruits and vegetables and impact of processing methods. Food and Nutrition Sciences, 6(03), 299.
Enneb, S., Drine, S., Bagues, M., Triki, T., Boussora, F., Guasmi, F., Ferchichi, A. (2020). Phytochemical profiles and nutritional composition of squash (Cucurbita moschata) from Tunisia. South African Journal of Botany, 130, 165-171.
Hayouni, E. A., Abedrabba, M., Bouix, M., and Hamdi, M. (2007). The effects of solvents and extraction method on the phenolic contents and biological activities in vitro of Tunisian Quercus coccifera L. and Juniperus phoenicea L. fruit extracts. Food chemistry, 105(3), 1126-1134.
Indrianingsih, A., Rosyida, V., Apriyana, W., Hayati, S. N., Nisa, K., Darsih, C., Indirayati, N. (2019). Comparisons of antioxidant activities of two varieties of pumpkin (Cucurbita moschata and Cucurbita maxima) extracts. Paper presented at the IOP Conference Series: Earth and Environmental Science.
Jacobo-Valenzuela N, de Jesus Zazueta-Morales J, Gallegos-Infante JA, Aguilar-Gutierrez F, Camacho-Hernandez IL, Rocha-Guzman NE, et al. (2011). Chemical and physicochemical characterization of winter squash (Cucurbita moschata). Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 39(1), 34-40.
Jayasundara, C., Deraniyagala, S. A., Hettiarachchi, C., and Thiripuranathar, G. (2018). Antioxidant and antibacterial activities of leaves, skin, flesh and seeds of Sri Lankan variety of cucurbita moschata. International Journal of Ayurveda and Pharma Research.
Jung, C.-H., Seog, H.-M., Choi, I.-W., Park, M.-W., and Cho, H.-Y. (2006). Antioxidant properties of various solvent extracts from wild ginseng leaves. LWT-Food Science and Technology, 39(3), 266-274.
Kartal, N., Sokmen, M., Tepe, B., Daferera, D., Polissiou, M., and Sokmen, A. (2007). Investigation of the antioxidant properties of Ferula orientalis L. using a suitable extraction procedure. Food chemistry, 100(2), 584-589.
Kaur, G. (2018). Development of Functionalprobiotic Beverage from Pumpkin. Lovely Professional University,
Kim, M. Y., Kim, E. J., Kim, Y.-N., Choi, C., and Lee, B.-H. (2012). Comparison of the chemical compositions and nutritive values of various pumpkin (Cucurbitaceae) species and parts. Nutrition research and practice, 6(1), 21-27.
Kim, S.-J., Cho, A. R., and Han, J. (2013). Antioxidant and antimicrobial activities of leafy green vegetable extracts and their applications to meat product preservation. Food control, 29(1), 112-120.
Krishnaiah, D., Sarbatly, R., and Nithyanandam, R. (2011). A review of the antioxidant potential of medicinal plant species. Food and bioproducts processing, 89(3), 217-233.
Kulczyński, B., and Gramza-Michałowska, A. (2016). Goji berry (Lycium barbarum): composition and health effects–a review. Polish Journal of Food and Nutrition Sciences, 66(2), 67-76.
Kulczyński, B., and Gramza-Michałowska, A. (2019a). The profile of carotenoids and other bioactive molecules in various pumpkin fruits (Cucurbita maxima Duchesne) cultivars. Molecules, 24(18), 3212.
Kulczyński, B., and Gramza-Michałowska, A. (2019b). The profile of secondary metabolites and other bioactive compounds in Cucurbita pepo L. and Cucurbita moschata pumpkin cultivars. Molecules, 24(16), 2945.
Kulczyński, B., Gramza-Michałowska, A., and Królczyk, J. B. (2020). Optimization of Extraction Conditions for the Antioxidant Potential of Different Pumpkin Varieties (Cucurbita maxima). Sustainability, 12(4), 1305.
Mala, K. S., and Kurian, A. E. (2016). Nutritional composition and antioxidant activity of pumpkin wastes. International Journal of Pharmaceutical, Chemical & Biological Sciences, 6(3).
Montesano, D., Blasi, F., Simonetti, M. S., Santini, A., and Cossignani, L. (2018). Chemical and nutritional characterization of seed oil from Cucurbita maxima(var. Berrettina) pumpkin. Foods, 7(3), 30.
Okwu, D. E., and Ohenhen, O. (2010). Isolation and characterization of Steroidal Glycosides from the leaves of Stachytarpheta Jamaicensis Linn Vahl. Der Chemica Sinica, 1(2), 6-14.
Ordonez, A., Gomez, J., and Vattuone, M. (2006). Antioxidant activities of Sechium edule (Jacq.) Swartz extracts. Food chemistry, 97(3), 452-458.
Pandey, S., Singh, J., Upadhyay, A., Ram, D., and Rai, M. (2003). Ascorbate and carotenoid content in an Indian collection of pumpkin (Cucurbita moschata ex Poir.). Cucurbit Genetics Cooperative Report, 26, 51-53.
Ramroudi, M., Kazemitabar, S. K., Esmaeilzadeh Kenari, R., and Najafi Zarini, H. (2020). Evaluation of Antioxidant Activity of Hydroalcoholic Extracts from Pumpkin (Cucurbita moschata) Seed, Flesh and Skin. Iranian Journal of Biosystems Engineering, 51(3), 663-672.
Rane, Z., Anish-Kumar, P., and Bhaskar, A. (2012). Phytochemical evaluation by GC-MS and in vitro antioxidant activity of Punica granatum fruit rind extract. Journal of Chemical and Pharmaceutical Research, 4(6), 2869-2873.
Rathi, M., and Abdulhay, H. (2018). Total phenolic contents and antioxidant activity of extracts of Tea (Black, Green and White). Asian Jr. of Microbiol. Biotech. Env. Sc, 20(2018), 45-49.
Rezig, L., Chouaibi, M., Meddeb, W., Msaada, K., and Hamdi, S. (2019). Chemical composition and bioactive compounds of Cucurbitaceae seeds: Potential sources for new trends of plant oils. Process Safety and Environmental Protection, 127, 73-81.
Sauer, M., Porro, D., Mattanovich, D., and Branduardi, P. (2008). Microbial production of organic acids: expanding the markets. Trends in biotechnology, 26(2), 100-108.
Singh, J., Singh, V., Shukla, S., and Rai, A. (2016). Phenolic content and antioxidant capacity of selected cucurbit fruits extracted with different solvents. J Nutr Food Sci, 6(6), 1-8.
Singh, S., Singh, R., Thakur, P., and Kumar, R. (2018). Phytochemicals, functionality and breeding for enrichment of cole vegetables (Brassica oleracea). Phytochemicals in vegetables: a valuable source of bioactive compounds. Bentham Science Publishers, UAE, 256-295.
Stevenson, D. G., Eller, F. J., Wang, L., Jane, J.-L., Wang, T., and Inglett, G. E. (2007). Oil and tocopherol content and composition of pumpkin seed oil in 12 cultivars. Journal of agricultural and food chemistry, 55(10), 4005-4013.
Vale, A., Santos, J., Melia, N., Peixoto, V., Brito, N., and Oliveira, M. B. P. (2015). Phytochemical composition and antimicrobial properties of four varieties of Brassica oleracea sprouts. Food Control, 55, 248-256.
Wang, X., Wang, C., Zha, X., Mei, Y., Xia, J., and Jiao, Z. (2017). Supercritical carbon dioxide extraction of β-carotene and α-tocopherol from pumpkin: a Box–Behnken design for extraction variables. Analytical Methods, 9(2), 294-303.
Wu, H., Zhu, J., Diao, W., and Wang, C. (2014). Ultrasound-assisted enzymatic extraction and antioxidant activity of polysaccharides from pumpkin (Cucurbita moschata). Carbohydrate polymers, 113, 314-324.
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