M. Zandi Articles List

List of Articles M. Zandi

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1 - Comparative Expression Analysis of Spermatogonial Stem Cell Markers in Cattle and Sheep
F. Nasri Ahangar M. Zandi M.R. Sanjabi A. Ghaedrahmati

Spermatogenesis is supported by stem cells called spermatogonial stem cells (SSCs), which are capable of transmitting information to the next generation. However, little is known on the specific markers of SSCs in farm animals. We investigated the expression of cdh1, cm More

Spermatogenesis is supported by stem cells called spermatogonial stem cells (SSCs), which are capable of transmitting information to the next generation. However, little is known on the specific markers of SSCs in farm animals. We investigated the expression of cdh1, cmyc, bcl6b, plzf, gfra1, nanog, vasa, thy1 and uchl1, as specific markers of SSCs, in bovine and ovine SSCs. The expression of the studied genes was conducted by real-time polymerase chain reaction method. For this reason, the enzymatic digestion process for two times was used to achieve SSCs from male calves and ram lambs testes. Then, filtration and differential plating techniques increased the SSC number in the cell suspension caused by mechanical and enzymatic digestions. Sertoli cells treated with Mitomycin-C were applied to obtain the feeder layer. Culturing of the stem cells was done on Sertoli cell feeder layer. Our results revealed that the expression of nanog and plzf was similar in bovine and ovine SSCs. Unlike bovine SSC colonies, cdh1 gene was not expressed in colony of ovine SSCs and its use as a specific marker of sheep SSCs is not suggested. The expression of uchl, vasa, thy1 and cdh1 genes was significantly higher in bovine SSCs and the bcl6b and cmyc expression was significantly higher in ovine SSCs compared to each other (P<0.05). From the results of this study nanog, plzf, uchl1, vasa, thy1 and cdh1 are suggested as markers of bovine and nanog, plzf, bcl6b and cmyc genes as markers of ovine SSCs. Manuscript profile
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2 - Contribution of All Single Nucleotide Polymorphisms (SNPs) and Minor Allele Frequency Groups to Genetic Variation of Quantitative Traits in Suffolk Sheep
A. Taheri Yeganeh M.R. Sanjabi J. Fayazi M. Zandi J. Van der Werf

Accurate estimation of genetic and non-genetic variance components with pedigree and genomic information is essential for the correct prediction of breeding values. Single nucleotide polymorphisms (SNPs) from Australian Suffolk sheep were used in this research (50K illu More

Accurate estimation of genetic and non-genetic variance components with pedigree and genomic information is essential for the correct prediction of breeding values. Single nucleotide polymorphisms (SNPs) from Australian Suffolk sheep were used in this research (50K illumine). The characteristics of birth weight, weaning weight, length, and diameter of the wool warp were investigated. To study the relationship between allelic frequency and the amount of additive genetic variance explained. The SNPs were classified into five different groups of rare allelic frequency (MAF). Two statistical models were fitted a separate and joint analysis of each SNPs group statistical analyses were performed with the Bayesian method using the Gibbs sampling technique and the RKHS model (semiparametric method). The amount of genomic heritability estimated by all SNPs in the Bayesian approach were estimated as 0.46, 0.19, 0.75, and 0.48 for the traits of birth weight, weaning weight, length, and wool diameter, respectively. The total heritability estimated for different groups of rare allele frequencies, in the combined analysis were almost similar to the value obtained from all SNPs for all traits. Although the numbers of SNPs in different groups were similar, the amounts of genetic variance explained by MAF groups were different. For the two traits of birth weight and weaning weight, the first group with an allelic frequency of 0.01-0.1 had the highest amount of genomic heritability. The amount of genomic heritability of fibre diameter was variable in five MAF groups. The highest estimated value in the fifth group with rare allele frequency was 0.4-0.5 (about 0.21), and the lowest value was in the third group (0.2-0.3), which was estimated at 0.098. The staple length trait, the genetic variance distribution pattern justified by SNPs, fluctuated between the five MAF groups, and the heritability value varied from zero in the second and fourth groups to about 0.16 in the third group. Manuscript profile
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3 - The Developmental Competence of the Bovine Embryos Processed in IVC-Stroebech Medium vs. Mcr2aa Medium
M. Zandi A. Ghaedrahmati L. Stroebech

Fetal bovine serum (FBS) is preferred for its ability to provide a variety of molecules that can support the growth of embryos. However, the use of serum can lead to the development of large offspring syndrome, which is a significant problem. This study aimed to examine More

Fetal bovine serum (FBS) is preferred for its ability to provide a variety of molecules that can support the growth of embryos. However, the use of serum can lead to the development of large offspring syndrome, which is a significant problem. This study aimed to examine bovine embryo development derived from in vitro fertilized oocytes in two culture media: IVC-Stroebech medium, a chemically defined medium; and the modified medium of Charles Rosenkrans with amino acids (mCR2aa), an undefined medium containing serum. The process of in vitro maturation was carried out at 38.5 ˚C in IVC-Stroebech medium. The process of in vitro fertilization was carried out for 18 hours using Bracket and Oliphant's (BO) medium. As to the experiment, cumulus-free presumptive zygotes were randomly assigned to two different culture medium treatments. The first treatment was a two-step culture system using mCR2aa medium. The first step in-volved using mCR2aa medium without FBS for the first 48 hours of culture. After that, for the remaining days of culture, the second-step mCR2aa, which is composed of 10% FBS, replaced the medium. The em-bryos were incubated at 38.5 ˚C under 5% CO2 5% O2 and 90% N2 for 10 days. Statistical analysis re-vealed a significantly higher hatched blastocyst rate in the IVC-Stroebech medium in comparison with the mCR2aa medium. There was not any significant difference (P>0.05) in the formation rate of morulas and blastocysts between the IVC-Stroebech medium and the mCR2aa medium. Findings recommended the use of IVC-Stroebech medium for bovine in vitro embryo culture. Manuscript profile

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List of Articles M. Zandi