اثرات تزریق داخل بطن مغزی و داخل صفاقی لیپوپلی ساکارید بر اخذ غذا و سطح کورتیزول خون در جوجه‌های گوشتی
محورهای موضوعی : نشانگر های زیستی پلاسما
سعید قیاسی
1
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مرتضی زنده‌دل
2
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هادی حق‌بین نظرپاک
3
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احمد اصغری
4
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نریمان شیخی
5
1 - گروه علوم درمانگاهی، دانشکده دامپزشکی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
2 - گروه علوم پایه، دانشکده دامپزشکی، دانشگاه تهران، تهران، ایران
3 - گروه علوم درمانگاهی، دانشکده دامپزشکی، واحد گرمسار، دانشگاه آزاد اسلامی، گرمسار، ایران
4 - گروه علوم درمانگاهی، دانشکده دامپزشکی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
5 - گروه علوم درمانگاهی، دانشکده دامپزشکی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
کلید واژه: لیپوپلی‌ساکارید, اخذ غذا, کورتیزول, جوجه‌های گوشتی,
چکیده مقاله :
زمینه و هدف: هدف از مطالعه کنونی، بررسی اثرات تزریق داخل بطن مغزی (ICV) و داخل صفاقی (IP) لیپوپلی ساکارید (LPS) بر اخذ غذا و سطح کورتیزول خون در جوجه‌های گوشتی می‌باشد.
مواد و روش‌ها: در مطالعه حاضر مجموعاً چهار آزمایش (آزمایش یک و دو مربوط به اخذ غذا و آزمایش سه و چهار مربوط به سطح کورتیزول پلاسما) بر روی جوجه‌های گوشتی اجرا شدند. در آزمایش اول محلول کنترل و LPS با دوزهای 25، 50 و 100 نانوگرم به صورت ICV تجویز شد. در آزمایش دوم نیز محلول کنترل و LPS با دوزهای 50، 100 و 200 میکروگرم به صورت IP تزریق گشت. سپس، جوجه‌ها به قفس‌هایشان بازگردانده شدند و میزان اخذ غذای آن‌ها تا سه ساعت پس از تزریق ثبت شد. ترتیب تزریقات در آزمایش‌های سه و چهار به ترتیب مشابه دو آزمایش اول بود. در این آزمایش‌ها دو ساعت پس از تزریق، با بریدن سیاهرگ گردنی، خونگیری انجام و سطح کورتیزول پلاسما در تمامی گروه‌ها سنجیده شد.
نتایج: بر اساس یافته‌ها، تزریق ICV 50 و 100 نانوگرم و تزریق IP 200 میکروگرم از LPS به طور معنی‌داری مصرف غذا را در 120 و 180 دقیقه پس از تزریق سرکوب کرد (05/0P≤ ). همچنین، تزریق ICV 50 و 100 نانوگرم و تزریق IP 100و 200 میکروگرم از LPS منجر به افزایش معنی‌دار سطح کورتیزول پلاسما شد (05/0P≤ ).
نتیجه‌گیری: این نتایج نشان می‌دهند که هم تجویز مرکزی و هم محیطی LPS می‌تواند مصرف غذا و پاسخ به استرس را در جوجه‌های گوشتی تعدیل کند.
Background & Aim: The present study aimed to investigate the effects of intraventricular (ICV) and intraperitoneal (IP) injections of lipopolysaccharide (LPS) on food intake and blood cortisol levels in broiler chickens.
Materials and Methods: In the present study, a total of four experiments (experiments one and two related to food intake and experiments three and four related to plasma cortisol levels) were performed on broiler chickens. In the first experiment, the control solution and LPS at doses of 25, 50, and 100 ng were administered ICV. In the second experiment, the control solution and LPS at doses of 50, 100, and 200 μg were injected IP. Then, the chickens were returned to their cages and their food intake was recorded for three hours after injection. The order of injections in experiments three and four was the same as in the first two experiments, respectively. In these experiments, two hours after injection, blood was collected by cutting the jugular vein, and plasma cortisol levels were measured in all groups.
Results: Based on the findings, ICV injection of 50 and 100 ng and IP injection of 200 μg of LPS significantly suppressed food intake at 120 and 180 minutes after injection (P≤0.05). Also, ICV injection of 50 and 100 ng and IP injection of 100 and 200 μg of LPS resulted in a significant increase in plasma cortisol levels (P≤0.05).
Conclusion: These results suggest that both central and peripheral administration of LPS can modulate food intake and stress responses in broiler chickens
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