Effects of intracerebroventricular and intraperitoneal lipopolysaccharide injection on food intake and blood cortisol levels in broiler chickens
Subject Areas : Plasma biomarkers
Saeed Ghiasi
1
,
Morteza Zendehdel
2
*
,
Hadi Haghbinnazarpak
3
,
Ahmad Asghari
4
,
Nariman Sheikhi
5
1 - Department of Clinical Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 -
3 - Department of Basic Sciences, Faculty of Veterinary Medicine, Garmsar Branch, Islamic Azad University, Garmsar, Iran
4 - Department of Clinical Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran
5 - Department of Clinical Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran
Keywords: Lipopolysaccharide, Food Intake, Cortisol, Broiler Chickens,
Abstract :
Background & Aim: The present study aimed to investigate the effects of intraventricular (ICV) and intraperitoneal (IP) injections of lipopolysaccharide (LPS) on food intake and blood cortisol levels in broiler chickens.
Materials and Methods: In the present study, a total of four experiments (experiments one and two related to food intake and experiments three and four related to plasma cortisol levels) were performed on broiler chickens. In the first experiment, the control solution and LPS at doses of 25, 50, and 100 ng were administered ICV. In the second experiment, the control solution and LPS at doses of 50, 100, and 200 μg were injected IP. Then, the chickens were returned to their cages and their food intake was recorded for three hours after injection. The order of injections in experiments three and four was the same as in the first two experiments, respectively. In these experiments, two hours after injection, blood was collected by cutting the jugular vein, and plasma cortisol levels were measured in all groups.
Results: Based on the findings, ICV injection of 50 and 100 ng and IP injection of 200 μg of LPS significantly suppressed food intake at 120 and 180 minutes after injection (P≤0.05). Also, ICV injection of 50 and 100 ng and IP injection of 100 and 200 μg of LPS resulted in a significant increase in plasma cortisol levels (P≤0.05).
Conclusion: These results suggest that both central and peripheral administration of LPS can modulate food intake and stress responses in broiler chickens
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