Investigation of the effect of Helicobacter pylori neutrophil-activating protein on leukotriene C4, B4 levels and airway inflammation in an allergic asthma mouse model.
Effect of Helicobacter neutrophil activator protein on C4, B4 and asthma inflammation
Subject Areas : Plasma biomarkers
Alireza Khaleghi khorrami
1
,
Rasoul Shokri
2
,
Seyyed Shamsadin Athari
3
,
Sanaz Mahmazi
4
1 -
2 - Biology Research Center, Zanjan Branch, Islamic Azad University, Zanjan, Iran
3 - Department of Immunology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
4 - Faculty of Basic, Technical and Engineering Sciences, Islamic Azad University, Zanjan Branch, Zanjan, Iran.
Keywords: Helicobacter pylori, allergic asthma, neutrophil activating protein, leukotriene,
Abstract :
Background & Aim: Helicobacter pylori neutrophil-activating protein (HP-NAP) acts as an immunogen to elicit antigenic responses or an immunomodulator to regulate T-cell immunity. This protein can lead to a Th2 response, which may result in an allergic response or a Th1 response. It is also likely to affect the production of leukotrienes C4, B4 and finally asthma. The objective of this study is to examine the impact of HP-NAP on the concentration of leukotrienes C4 and B4 in an allergic asthma model in mice and to compare this with the effects of Helicobacter pylori. Materials & Methods: Firstly, the Helicobacter pylori standard strain and HP-NAP peptide were prepared in accordance with the methodology outlined in a previous study. Subsequently, 40 adult Blab c 6 to 8-week-old male mice were prepared for modelling and developing allergic asthma and were divided into four groups of 10. These mice were selected for further study to investigate the changes in the aforementioned leukotrienes. Results: demonstrated that the level of leukotrienes C4 and B4, as measured using the ELISA method, was significantly elevated in the asthmatic group compared to the healthy group. While the treatments with Helicobacter and HP-NAP did not result in a significant decrease in the levels of C4 and B4 compared to the asthmatic group, Conclusion: In light of the more pronounced effects of HP-NAP compared to Helicobacter itself in reducing the level of C4 and B4 leukotrienes, HP-NAP was able to reduce the level of C4 and B4 leukotrienes in asthmatic animals. However, this reduction was not statistically significant when compared to the asthmatic group. Therefore, further studies are recommended.
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