مقایسه اثرات نانوذرات نقره، مس و اکسید روی بر بیان ژن فاکتور نکروز تومور α (TNF-α) در موش Balb/C آلوده به اشریشیا کلی
محورهای موضوعی : میکروب شناسی مولکولیمریم محمدخانی 1 , صبا طاهری 2 * , جواد آراسته 3
1 - گروه زیست شناسی، واحد تهران مرکزی، دانشگاه آزاد اسلامی، تهران، ایران.
2 - دانشکده علوم پایه، دانشگاه آزاد اسلامی واحد اسلامشهر، ایران.
3 - گروه زیست شناسی، واحد تهران مرکزی، دانشگاه آزاد اسلامی، تهران، ایران.
کلید واژه: Escherichia coli, نانوذرات, نقره, اکسید روی, مس, فاکتور نکروز توموری آلفا,
چکیده مقاله :
زمینه و اهداف: تعداد افراد مبتلا به باکتری‌های بیماری‌زا در حال افزایش است. آنتی‌بیوتیک‌های رایج به دلیل سازوکارهای ایجاد مقاومت، کارایی خود را از دست می‌دهند. فناوری نانو رویکردی به نام عوامل ضد میکروبی مبتنی بر نانوذرات معرفی کرده است که قادر به مهار رشد باکتری‌ها می‌باشد.
مواد و روش‌ها: حداقل غلظت مهارکنندگی (MIC) نانوذرات نقره (Ag) ، اکسید روی (ZnO) و مس (Cu) علیه Escherichia coli ATCC 25922 با استفاده از روش رقت‌سازی در کشت مایع تعیین شد. ۵۴ موش نر نژاد Balb/C به 9 گروه تقسیم شدند، به‌صورت داخل صفاقی با E.coli (CFU/mL 10^8)آلوده شده و با نانوذرات ZnO، Ag و Cu (در دز MIC) یا ایمی‌پنم (گروه کنترل) تحت درمان قرار گرفتند. ۲۴ ساعت پس از درمان، RNA طحال استخراج شد و بیان ژن فاکتور نکروز توموری آلفا (TNF-α) با استفاده از Real-time PCR سنجیده شد.
یافته‌ها: MIC نانوذرات Ag برابر ۱۲۵ میکروگرم بر میلی‌لیتر و برای ZnO و Cu برابر ۶۲.۵ میکروگرم بر میلی‌لیتر بود که اثربخشی بهتر نانوذرات ZnO و Cu را نشان داد. بیان ژن TNF-α در گروه‌های دریافت‌کننده ایمی‌پنم و نانوذرات نقره به‌طور معنی‌داری کمتر از گروه‌های دریافت‌کننده ZnO و Cu بود (p < 0.05).
نتیجه‌گیری: صرف‌نظر از آنتی‌بیوتیک‌ها، نانوذرات نیز خاصیت ضد میکروبی علیه باکتری‌هایی نظیر E. coli دارند. این ویژگی‌ها آن‌ها را به گزینه‌ای مناسب برای درمان بیماری‌های عفونی تبدیل می‌کند.
Background & Objectives: The number of people infected by pathogenic bacteria is increasing. Routine antibiotics lose their effectiveness due to the strategies of these bacteria in resistant to antibiotics. Nanotechnology has introduced an approach called nanoparticle-based antimicrobial agents to inhibit bacteria growth.
Materials & Method: Minimum inhibitory concentrations (MICs) of silver (Ag), zinc oxide (ZnO), and copper (Cu) NPs against Escherichia coli ATCC 25922 were determined using microbroth dilution. Balb/C male mice (n=54) were divided into nine groups, infected intraperitoneally with E. coli (10^8 CFU/mL), and treated with Ag, ZnO, or Cu NPs (MIC doses) or imipenem (control). Spleen tissue RNA was extracted 24 h post-treatment, and tumor necrosis factor-α (TNF-α) gene expression was quantified via Real-time PCR.
Results: MICs were 125 µg/mL (Ag) and 62.5 µg/mL (ZnO, Cu), indicating superior efficacy of ZnO and Cu NPs. TNF-α expression was significantly lower in imipenem and Ag NP groups compared to ZnO and Cu NP groups (p < 0.05).
Conclusion: Regardless of the antibiotics, NPs also exhibit an antimicrobial property against bacteria like E. coli. These characteristics make them the proper candidate for the treatment of infectious diseases.
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