بررسی امکان افزایش عملکرد فیزیولوژیکی گیاه دارویی Lippia citriodora L. با استفاده از محرک‌های زیستی در شرایط تنش شوری
محورهای موضوعی :
گیاهان دارویی
رضا دهقانی بیدگلی
1
1 - گروه مرتع وآبخیز دانشکده منابع طبیعی دانشگاه کاشان
تاریخ دریافت : 1397/07/21
تاریخ پذیرش : 1398/09/05
تاریخ انتشار : 1398/09/01
کلید واژه:
تنش شوری,
وزن خشک,
اسانس,
باکتری محرک رشد,
به لیمو,
چکیده مقاله :
تنش‌های محیطی جزء مهم‌ترین عوامل کاهش عملکرد گیاهان دارویی به‌شمار می‌روند و استفاده از مواد تنظیم‌کننده رشد گیاهان نظیر باکتری‌های محرک رشد می‌تواند راهکاری برای کاهش اثرات تنش شوری باشد. عوامل محیطی از یک سو باعث تغییراتی در رشد گیاهان دارویی و از طرف دیگر موجب تغییر در مقدار و کیفیت مواد موثره آن‌ها نظیر آلکالوئیدها، گلیکوزیدها، استروئیدها و روغن‌های فرار (اسانس‌ها) می‌گردند. این آزمایش به‌صورت فاکتوریل در قالب طرح پایه بلوک کامل تصادفی با 12 تیمار در 4 تکرار در گلخانه دانشگاه کاشان انجام شد. عامل اول تنش شوری بود 3 سطح شوری 25، 50 و 100 میلی مولار بود که تیمارهای شوری پس از اسقرار کامل پایه‌های کشت شده انجام گرفت. عامل دوم سویه باکتری محرک رشد در چهار سطح شامل شاهد (صفر) و افزودن 10-10، 8-10 و 6-10 مولار به خاک گلدان‌ها به‌صورت محلول قبل از اعمال تنش شوری بود. از نتایج بدست آمده مشخص شد که استفاده از ماده تنظیم‌کننده رشد گیاهی (باکتری محرک رشد) برای حفظ عملکرد اقتصادی گیاهان تحت تنش ضروری و قابل توجیه است. نتایج نشان داد که افزایش تنش شوری تاثیر معنی‌داری در کاهش پارامترهای رشد شامل وزن خشک، طول ریشه، ارتفاع گیاه و عملکرد اسانس داشت. براساس نتایج، درصد اسانس با افزایش سطح شوری، افزایش معنی‌داری را در سطح یک درصد نشان داد؛ به‌طوری‌که درصد اسانس از 45/0 در تیمار شوری 25 میلی مولار به 96/0 در تیمار 100 میلی مولار و استفاده از غلظت 8-10 مولار باکتری رسید.
چکیده انگلیسی:
Environmental stresses are one of the most important factors in reducing the function of medicinal plants. The use of plant growth regulators such as PGPR can be a way to reduce the effects of salinity stress. Environmental factors, cause changes in the growth of medicinal plants and, on the other hand, alter the amount and quality of their active substances, such as alkaloids, glycosides, steroids, volatile oils (essential oils). The experiment was conducted factorial based on a randomized complete block design with 12 treatments in four replications at greenhouse of University of Kashan. The first factor was salinity stress at 3 levels including 25, 50 and 100 (MM) Mili molar and salinity treatments were performed after complete sowing of cultivated plants. The second factor was bacterial growth stimulus in four levels including control (0), 10-10, 10-8 and 10-6 molar and the addition to the potted soil as a solution before applying salinity stress. It was found that the use of plant growth regulator PGPR is essential for maintaining the economic performance of plants under stress. The results showed that increased levels of salinity had a significant effect on reducing the growth parameters including dry weight, root length, plant height and essential oil yield. According to the results, the percentage of essential oil with increased levels of salinity significantly at 1% level, so that the percentage of essential oil from 0.45 in 25 (MM) treatment was increased to 0.96 in 100(MM) and the use of 10-8 M PGPR.
منابع و مأخذ:
References
Aktas, H., Abak, K., Oztark, L. and Cakmak, I. 2016. The effect of PGPRs on growth and shoot concentrations of phosphor and potassium in wheat and barley cultivars under drought stress. Turkish Journal of Agriculture and Forestry, 30: 407-41.
Argyropoulou, C.C, Daferera, D. and Tarantilis, P.A. 2014. Chemical composition of the essential oil from leaves of Lippia citriodora (Verbenaceae) in salinity stress. Journal of Plant and Soil. 15: 14–20.
Argyropoulou, C.C., Daferera, D. and Tarantilis, P.A. 2017. Chemical composition of the essential oil from leaves of Lippia citriodora (Verbenaceae) at two developmental stages. Journal of Plant Genom. 35: 31–37.
Aydin, A. and Metin, T. 2012. Humic acid application alleviates salinity stress of bean (Phaseolus vulgaris L.) plants decreasing membrane leakage. African Journal of Agricultural Research. 7(7): 1073-1086.
Bettaieb, I., Zakhama, N., Wannes, W.A., Kchouk, M.E. and Marzouk, B. 2008. Water deficit effects on Salvia officinalis fatty acids and essential oils composition. Science Journal of Horticulture. 120 (2): 271-275.
Boyer, J.S. 1982. Plant productivity and environment. Science, 218: 443-448.
Barassi, C.A., Ayrault, G., Creus, C.M., Sueldo, R.J. and Sobero, M.T. 2018. Seed inoulation with Azospirillum mitigates NaCl effects on lettuce. Journal of Horticulture. 109: 8-14.
Clomse, S.D., Zurek, D.M., McMorris, T.C. and Baker, M.E. 2014. Effect of brassinolide on gene expression in elongating soybean epicotyls. Journal of Plant Physiology. 100: 1377-1388.
Dambolena, J.S., Zunino, M.P., Lucini,
E.I., Olmedo, R., Banchio, E., Bima, P.J. and Zygadlo, J.A. 2017. Total phenolic content, radic alscavenging properties and essential oil composition of Origanum species from different populations. Journal of Agricultural and Food Chemistry, 58: 1115-1120.
Demir Kaya, M., Gamze, O. and Yakup, M.C. 2016. Seed treatments to overcome salt and drought stress during germination in sunflower (Helianthus annuus L.). European Journal of Agronomy. 24: 291-295.
Denis Thomas, T. 2018. The role of activated charcoal in plant tissue culture. Journal of Biotechnology Advances. 26: 618–31.
El-Keblawy, A. and Hassan, N. 2006. Salinity, temperature and light affects see of Haloxylon salicornium: acommon plant in sandy habitats Arabian Desert. International Symposium in Drylands, Ecology and Human Security, Dubai, United Arab Emirates, Pp: 15-32.
El-Sherif, A.F., Shehata, S.M. and Youssif, R.M. 1990. Response of tomato seedlings to zinc application under different salinity levels. Egyptian Journal of Horticulture, 17(1): 131-142.
Enjavi, F., Taghvaei, M., Sadeghei, H. and Hassanli, H. 2015. Effects of superabsorbent polymer on early vigor and wate use efficiency of (Calotropis procera L.) seedlings under drought stress. Iranian Journal of Range and Desert Research, 22(2): 216-230. (In Persian).
Hendawy, S.F. and Khalid, K.A. 2005. Response of sage Salvia officinalis L. plants to zinc application under different salinity levels. Journal of Applied Science Research. 1(2): 147-155.
Ingram, J. and Bartels D. 1996. The molecular basis of dehydration tolerance in plants. Journal of Plant Physiology. 47: 377-403.
Inze, D. and Montag, M.V. 2000. Oxidative stress in plants. TJ International Ltd, Padstow, Carnawell. Great Britain, 321 p.
Jalili, F., Khavazi, K. and Asadi Rahmani, H. 2019. Effect of fluorescent Pseudomonas bacteria on plant growth and nutrient uptake of canola grown in saline conditions. In: 11th Iranian Soil Sciences Congress Soil Management& Food Security, Gorgan, Iran. P: 77-78
Jarosova, M., Klejdus, B., Kovacik, J., Babula, P. and Hedbavny, J. 2016. Humic acid protects barley against salinity. Journal of Acta Physiologiae Plantarum, 38(6):1-9.
Khaled, H. and Fawy, H.A. 2011. Effect of different levels of humic acids on the nutrient content, plant growth, and soil properties under conditions of salinity. Journal of Soil and Water Research. 6(3): 21–29.
Kulikova, N.A., Stepanova, E.V. and Koroleva, O.V. 2015. Mitigating activity of humic substances: Direct Influence on Biota. In: Use of Humic Substances to Remediate Polluted Environments: From Theory to Practice, NATO Science Series IV: Erath and Environmental Series, Perminova, I.V. (Eds). Kluwer Academic Publishers, USA, pp. 285- 309.
Kusano, T., Berberich, T., Tateda, C. and Takahashi, Y. 2008. Polyamines: essential factors for growth and survival. Journal of Planta, 228: 367-381.
Leithy, S., El-Meseiry, T.A. and Abdallah, E.F. 2018. Effect of biofertilizers, cell stabilizer and irrigation regime on rosemary herbage oil yield and quality. Journal of Applied Sciences Research. 2(10): 773-9.
Li, K.R., Wang, H.H., Han, G., Wang, Q. and Fan, J. 2008. Effects of brassinolide on the survival, growth and drought resistance of Robinia pseudoacacia seedlings under water-stress. Journal of New Forests. 35: 255-266.
Marcelo, A.G., Saul, B., Okon, Y. and Jaime, K. 2017. Effects of Azospirillum brasilense on root morphology of common bean (Phaseolus vulgaris L.) under different water regimes. Journal of Biology and Fertilizer Soils. 32: 259-64.
Manieval, S., Thierry, D. and Christiane, G. 2001. Relationship between biomass and phenolic in grain Sorghum grown under different conditions. Agronomy Journal. 93: 49-54.
Mozafarian, V.A. 1998. Culture of plants name of Iran. Contemporary Culture, 435 p. (In Persian).
Omidbaigi, R. 2004. Production and processing of medicinal plants. Publication of Astan Qods Razavi. (In Persian).
Rahmani, N., Aliabadi Farahani, H. and Valadabadi, S.A.R. 2008. Effects of nitrogen on oil yield and its component of Calendula (Calendula officinalis L.) in drought stress conditions. Abstracts Book of The world congress on medicinal and aromatic plants, South Africa, 364p.
Sardashti, A.R., Valizadeh, J. and Adhami Y. 2013. Variation in the essential oil composition of Perovskia abrotanoides of different growth stage in Baluchestan. Middle East. Journal of Scientific Research. (6): 781-4.
Schilling, G., Schiller, C. and Otto S. 1991. Influence of brassinosteroids on organ relations and enzyme activities of sugar-beet plants. In Brassinosteroids. Chemistry, Bioactivity, and Applications. ACS Symposium Series. American Chemical Society, Washington, 474: 208-219.
Sofo, A., Tuzio, A.C., Dichio, B. and Xiloyannis, C. 2005. Influence of water deficit and rewatering on the components of the ascorbate-glutathione cycle in four interspecific Prunus hybrids. Journal of Plant Science. 69: 403-412.
Spector, E. 2012. Lemon Herbs: How to grow and use 18 great plants, Stackpol Books. USA, p. 35.
Swamy, K., Ram, N. and Rao, S. 2017. Influence of 28-homobrassinolid on growth, photosynthesis metabolite and essential oil of geranium. American Journal of Plant Physiology. 3(4):173-179.
Yildirim, E. and Taylor, A.G. 2019. Effect of biological treatments on growth of bean plans under salt stress. Journal of Botany. 48(3): 176-177.
Tarfias, S. and Ibrahim, M. 2016. Physiological response of rosemary, Rosmarinus officinalis L. plant to brassinosteroid and uniconazole. Cultivation and Production of Medicinal and Aromatic Plants, 230 p.
Zargari, A. 2006. Medicinal Plants. University of Tehran Press. 570p. (In Persian).
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References
Aktas, H., Abak, K., Oztark, L. and Cakmak, I. 2016. The effect of PGPRs on growth and shoot concentrations of phosphor and potassium in wheat and barley cultivars under drought stress. Turkish Journal of Agriculture and Forestry, 30: 407-41.
Argyropoulou, C.C, Daferera, D. and Tarantilis, P.A. 2014. Chemical composition of the essential oil from leaves of Lippia citriodora (Verbenaceae) in salinity stress. Journal of Plant and Soil. 15: 14–20.
Argyropoulou, C.C., Daferera, D. and Tarantilis, P.A. 2017. Chemical composition of the essential oil from leaves of Lippia citriodora (Verbenaceae) at two developmental stages. Journal of Plant Genom. 35: 31–37.
Aydin, A. and Metin, T. 2012. Humic acid application alleviates salinity stress of bean (Phaseolus vulgaris L.) plants decreasing membrane leakage. African Journal of Agricultural Research. 7(7): 1073-1086.
Bettaieb, I., Zakhama, N., Wannes, W.A., Kchouk, M.E. and Marzouk, B. 2008. Water deficit effects on Salvia officinalis fatty acids and essential oils composition. Science Journal of Horticulture. 120 (2): 271-275.
Boyer, J.S. 1982. Plant productivity and environment. Science, 218: 443-448.
Barassi, C.A., Ayrault, G., Creus, C.M., Sueldo, R.J. and Sobero, M.T. 2018. Seed inoulation with Azospirillum mitigates NaCl effects on lettuce. Journal of Horticulture. 109: 8-14.
Clomse, S.D., Zurek, D.M., McMorris, T.C. and Baker, M.E. 2014. Effect of brassinolide on gene expression in elongating soybean epicotyls. Journal of Plant Physiology. 100: 1377-1388.
Dambolena, J.S., Zunino, M.P., Lucini,
E.I., Olmedo, R., Banchio, E., Bima, P.J. and Zygadlo, J.A. 2017. Total phenolic content, radic alscavenging properties and essential oil composition of Origanum species from different populations. Journal of Agricultural and Food Chemistry, 58: 1115-1120.
Demir Kaya, M., Gamze, O. and Yakup, M.C. 2016. Seed treatments to overcome salt and drought stress during germination in sunflower (Helianthus annuus L.). European Journal of Agronomy. 24: 291-295.
Denis Thomas, T. 2018. The role of activated charcoal in plant tissue culture. Journal of Biotechnology Advances. 26: 618–31.
El-Keblawy, A. and Hassan, N. 2006. Salinity, temperature and light affects see of Haloxylon salicornium: acommon plant in sandy habitats Arabian Desert. International Symposium in Drylands, Ecology and Human Security, Dubai, United Arab Emirates, Pp: 15-32.
El-Sherif, A.F., Shehata, S.M. and Youssif, R.M. 1990. Response of tomato seedlings to zinc application under different salinity levels. Egyptian Journal of Horticulture, 17(1): 131-142.
Enjavi, F., Taghvaei, M., Sadeghei, H. and Hassanli, H. 2015. Effects of superabsorbent polymer on early vigor and wate use efficiency of (Calotropis procera L.) seedlings under drought stress. Iranian Journal of Range and Desert Research, 22(2): 216-230. (In Persian).
Hendawy, S.F. and Khalid, K.A. 2005. Response of sage Salvia officinalis L. plants to zinc application under different salinity levels. Journal of Applied Science Research. 1(2): 147-155.
Ingram, J. and Bartels D. 1996. The molecular basis of dehydration tolerance in plants. Journal of Plant Physiology. 47: 377-403.
Inze, D. and Montag, M.V. 2000. Oxidative stress in plants. TJ International Ltd, Padstow, Carnawell. Great Britain, 321 p.
Jalili, F., Khavazi, K. and Asadi Rahmani, H. 2019. Effect of fluorescent Pseudomonas bacteria on plant growth and nutrient uptake of canola grown in saline conditions. In: 11th Iranian Soil Sciences Congress Soil Management& Food Security, Gorgan, Iran. P: 77-78
Jarosova, M., Klejdus, B., Kovacik, J., Babula, P. and Hedbavny, J. 2016. Humic acid protects barley against salinity. Journal of Acta Physiologiae Plantarum, 38(6):1-9.
Khaled, H. and Fawy, H.A. 2011. Effect of different levels of humic acids on the nutrient content, plant growth, and soil properties under conditions of salinity. Journal of Soil and Water Research. 6(3): 21–29.
Kulikova, N.A., Stepanova, E.V. and Koroleva, O.V. 2015. Mitigating activity of humic substances: Direct Influence on Biota. In: Use of Humic Substances to Remediate Polluted Environments: From Theory to Practice, NATO Science Series IV: Erath and Environmental Series, Perminova, I.V. (Eds). Kluwer Academic Publishers, USA, pp. 285- 309.
Kusano, T., Berberich, T., Tateda, C. and Takahashi, Y. 2008. Polyamines: essential factors for growth and survival. Journal of Planta, 228: 367-381.
Leithy, S., El-Meseiry, T.A. and Abdallah, E.F. 2018. Effect of biofertilizers, cell stabilizer and irrigation regime on rosemary herbage oil yield and quality. Journal of Applied Sciences Research. 2(10): 773-9.
Li, K.R., Wang, H.H., Han, G., Wang, Q. and Fan, J. 2008. Effects of brassinolide on the survival, growth and drought resistance of Robinia pseudoacacia seedlings under water-stress. Journal of New Forests. 35: 255-266.
Marcelo, A.G., Saul, B., Okon, Y. and Jaime, K. 2017. Effects of Azospirillum brasilense on root morphology of common bean (Phaseolus vulgaris L.) under different water regimes. Journal of Biology and Fertilizer Soils. 32: 259-64.
Manieval, S., Thierry, D. and Christiane, G. 2001. Relationship between biomass and phenolic in grain Sorghum grown under different conditions. Agronomy Journal. 93: 49-54.
Mozafarian, V.A. 1998. Culture of plants name of Iran. Contemporary Culture, 435 p. (In Persian).
Omidbaigi, R. 2004. Production and processing of medicinal plants. Publication of Astan Qods Razavi. (In Persian).
Rahmani, N., Aliabadi Farahani, H. and Valadabadi, S.A.R. 2008. Effects of nitrogen on oil yield and its component of Calendula (Calendula officinalis L.) in drought stress conditions. Abstracts Book of The world congress on medicinal and aromatic plants, South Africa, 364p.
Sardashti, A.R., Valizadeh, J. and Adhami Y. 2013. Variation in the essential oil composition of Perovskia abrotanoides of different growth stage in Baluchestan. Middle East. Journal of Scientific Research. (6): 781-4.
Schilling, G., Schiller, C. and Otto S. 1991. Influence of brassinosteroids on organ relations and enzyme activities of sugar-beet plants. In Brassinosteroids. Chemistry, Bioactivity, and Applications. ACS Symposium Series. American Chemical Society, Washington, 474: 208-219.
Sofo, A., Tuzio, A.C., Dichio, B. and Xiloyannis, C. 2005. Influence of water deficit and rewatering on the components of the ascorbate-glutathione cycle in four interspecific Prunus hybrids. Journal of Plant Science. 69: 403-412.
Spector, E. 2012. Lemon Herbs: How to grow and use 18 great plants, Stackpol Books. USA, p. 35.
Swamy, K., Ram, N. and Rao, S. 2017. Influence of 28-homobrassinolid on growth, photosynthesis metabolite and essential oil of geranium. American Journal of Plant Physiology. 3(4):173-179.
Yildirim, E. and Taylor, A.G. 2019. Effect of biological treatments on growth of bean plans under salt stress. Journal of Botany. 48(3): 176-177.
Tarfias, S. and Ibrahim, M. 2016. Physiological response of rosemary, Rosmarinus officinalis L. plant to brassinosteroid and uniconazole. Cultivation and Production of Medicinal and Aromatic Plants, 230 p.
Zargari, A. 2006. Medicinal Plants. University of Tehran Press. 570p. (In Persian).
