تاثیر نوع بستر کشت و اسید هیومیک بر برخی صفات مورفوفیزیولوژیک و مصرف آب گیاه سدوم دم عقربی(Carpobrotus edulis L.) روی یک سیستم بام سبز
محورهای موضوعی : ژنتیکسیده ملیحه ربانی خیرخواه 1 , فاطمه کاظمی 2
1 - گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران
2 - گروه باغبانی و فضای سبز ، دانشکده کشاورزی ، دانشگاه فردوسی مشهد، ایران
کلید واژه: نیاز آبی, کارایی مصرف آب, پوشش‌دهی گیاه, سیستم بام سبز, نتونیت,
چکیده مقاله :
سلامت گیاهان درشرایط بام سبز آن تا حد زیادی وابسته به تامین آب مورد نیازگیاهان است. ترکیبات بستر کشت، در حفظ و در دسترس قرار گرفتن رطوبت برای گیاه موثرند و یکی از راهکارها جهت کاهش مصرف آب در بام سبز، اصلاح بستر کشت است. در این پژوهش، نیاز آبی و برخی صفات مورفوفیزیولوژیک گیاه سدوم دم عقربی (Carpobrotus edulis L.) تحت تیمارهای بستر کشت و مقدار اسید هیومیک بررسی گردید. این تحقیق درقالب آزمایش فاکتوریل بر پایه طرح بلوک کامل تصادفی با سه تکرار از اردیبهشت تا شهریور 1399 به صورت کشت در جعبه‌های کشت در شرایط بام سبز در شهر مشهد انجام شد. عامل اول بسترهای کشت (شامل بستر کشت حاوی خاک شامل بستر کشت 1 (خاک 20%+20% لیکا+20% پرلیت+ پوکه معدنی+20% خاکبرگ)، بستر کشت 2 (بستر کشت1+ 6% بنتونیت) و بستر کشت 3 (بستر کشت 1+ 12% وزنی بنتونیت) و بسترهای کشت بدون خاک شامل بستر کشت 4 (پوکه معدنی 27%+ 27% لیکا+ 27% پرلیت+ 20 درصد خاکبرگ)، بستر کشت 5 (بستر کشت4+ 6% بنتونیت) و بستر کشت 6 (بستر کشت 4+ 12% وزنی بنتونیت) و عامل دوم غلظت اسید هیومیک (شامل صفر ، 100 و 200 میلی‌گرم در لیتر) بود و اسید هیومیک هر 15 روز به صورت کود آبیاری به گیاهان داده شد. نتایج نشان داد بسترهای حاوی خاک به طور قابل‌توجهی نسبت به بسترهای بدون خاک بهتر عمل کردند. بالاترین میزان پوشش‌دهی گیاهان و طول و حجم ریشه در بستر کشت 3 (حاوی خاک و 12% وزنی بنتونیت) رویت شد. بالاترین محتوی آب برگ و کلروفیل و کمترین کمبود اشباع نسبی، مقادیر کارتنوئید، فنول و کربوهیدرات برگ در بستر کشت 3 (حاوی خاک و 12% وزنی بنتونیت) مشاهده شد. تیمار 200 میلی‌گرم بر لیتر اسید هیومیک با بهترین عملکرد رشدی و فیزیولوژیکی گیاهان همراه بود. تیمار خاک 20%+20% لیکا+20% پرلیت+ پوکه معدنی+20% + 12% وزنی بنتونیت همراه با 200 میلی‌گرم بر لیتر اسید هیومیک، گیاهانی با مصرف بهینه آب حاصل آورد. وجود خاک و سوپرجاذب معدنی بنتونیت در بستر کشت بام سبز از نظر نگه داشت رطوبت اهمیت ویژه داشته و البته تغذیه تکمیلی گیاهان در بام سبز نیز موجب بهبود عملکرد گیاهان در بام سبز می‌گردد.
The health of plants in green roof conditions depends to a large extent on the supply of water required by plants. The composition of the substrate is effective in maintaining the moisture and making it available to the plant, and one of the solutions to reduce water consumption in the green roof is improving the substrate. In this study, water requirement and some morphophysiological traits of Carpobrotus edulis L. were studied under substrate and humic acid treatment. The research was conducted in green roof box conditions as a factorial experiment based on a randomized complete block design with three replications from May to September 2020 in Mashhad. The first factor was substrate including Substrate 1 (soil 20% + 20% Leica + 20% perlite + mineral pumice + 20% leaf soil), Substrate 2 (substrate 1 + 6% by weight of bentonite), Substrate 3 (substrate 1+ 12% by weight of bentonite), soilless Substrate 4 (27% mineral pumice + 27% Leica + 27% perlite + 20% leaf soil), Substrate 5 (substrate 4 + 6% by weight bentonite), and Substrate 6 (substrate 4+ 12% by weight of bentonite). The second factor was the concentration of humic acid (0, 100, and 200 mg L-1) applied as fertigation every 15 days. Soil-containing substrates performed significantly better than soilless substrates and the highest coverage, root length, and volume of green roof plants were observed in substrate 3 (containing soil and 12% by weight of bentonite). The highest leaf water and chlorophyll contents and the lowest relative saturation deficit, carotenoids, phenols, and leaf carbohydrates were observed in Substrate 3, which show the physiological stability of the plant under stress conditions, was evident in substrate 3. Application of 200 mg/L humic acid was associated with the best growth and physiological performance of plants. A substrate rich in moisture and nutrients, including 20% Soil + 20% Leica + 20% perlite +20% mineral pumice + 12% w bentonite + 200 mg/L humic acid resulted in the plants with optimal water consumption. The presence of soil and mineral superabsorbent (bentonite) in the green roof substrate is especially important in terms of moisture retention, and of course, the supplementary nutrition of plants in the green roof also improves their performance in the green roof.
Abedini, T., Moradi, P. and Hani, A. (2015). Effect of organic fertilizer and foliar application of humic acid on some quantitative and qualitative yield of Pot marigold. Journal of Novel Applied Sciences.10:1100-1103.
Abedi Kopaei, J. and Sohrab, F. (2004). Effect of zeolite and bentonite minerals on soil hydraulic properties. Proceedings of the 12th Iranian Conference on Crystallography and Mineralogy, Shahid Chamran University, Ahvaz, page 567. (In Persian with English Summary).
Allahdadi, A.B., Ghamsari, M., Akbari, G.A. and Zohourmehr, M. (2005). Study the effect of different levels of super absorbent polymer and irrigation on corn (Zea mays L.) growth and yield. 3th Congress on Super Absorbent Hydrogel Application in Agriculture. Research Center for Polymer and Petrochemical of Iran. (In Persian with English Summary).
Arancon, NQ, Yardin E, Edwards, CA. and Lee, S. (2003). The trophic diversity of nematode communities in soils treated with vermicomposts. Pedobiologia. 47: 731-735.
Arnon, D.S. )1940(. Copper enzyme in isolated chloroplast polyphenol oxidase in Beta Vulgaris. J. Plant Physiol. 24: 1-15.
Barrs, H. D. and Weatherley, P. E. (1962). A reexamination of the relative turgidity technique for the estimating of water deficits in leaves. Australian Journal of Biological Sciences. 15: 413-428.
Baryła, A., Gnatowski, T., Karczmarczyk, A. and Szatylowicz, J. (2019). Changes in temperature and moisture content of an extensive-type green roof. Sustainability. 11: 2498.
Bevilacqua, P., Coma, J., Pérez, G., Chocarro, C., Juárez, A., Solé, C., De Simone, M. and Cabeza, L. F. (2015). Plant cover and floristic composition effect on thermal behaviour of extensive green roofs. Building and Environment. 92: 305–316. doi:10.1016/j.buildenv.2015.04.026.
Blum, A. and Ebercon, A. )1981(. Cell membrane stability as a measure of drought and heat tolerance in wheat. Crop Science. 21:43-47.
Bianchini, F. and Hewage, K. (2012). How green are the green roofs? Lifecycle analysis of green roof materials. Building and Environment. 48:57–65.
Bidgoli, Z. (2016). Investigation of the use of superabsorbent in the amount of water retention on the green roof. Master Thesis. Faculty of Agriculture, University of Zanjan (In Persian).
Cacco, G., Attina, E., Gelsomino, A. and Sidari, M. (2000). Effect of nitrate and humic substances of different molecular size on kietic parameters of nitrate uptake in wheat seedlings. Journal of Plant Nutrition and Soil Science’s. 163: 313-320.
Calderin-Garcia, A., de Souza, L.G.A., Pereira, M.G., Castro, R.N., Garcia-Mina, J.M., Zonta, E., Lisboa, F.J.G. and Berbara, R.L.L. (2016). Structure-property-function relationship in humic substances to explain the biological activity in plants. Scientific Reports.6: 20798.
Canellas, L., Olivares, F., Aguiar, N., Jones, D., Nebbioso, A., Mazzei, P. and Piccolo, A. (2015). Humic and fulvic acids as biostimulants in horticulture. Scientia Horticulturae. 196: 15-27.
Cascone, S. (2019). Green Roof Design: State of the Art on Technology and Materials. Sustainability. 11: 3020, 1-28. doi:10.3390/su11113020.
Dalvand, M. (2016). The effect of humic acid on seed germination, growth indices and drought stress in parsley, Master Thesis, Faculty of Agriculture, Arak University (In Persian).
Deenavarman, M. , Lourdusamy, D. K. , Thangaselvabai T. and Venkatesan, K. (2018). "Effect of different media incorporated with Pusa hydrogel on growth and watering frequency of potted foliage plant, Arrowhead (Syngonium podophyllum Schott.). Journal of Agriculture and Ecology. pp. 71-76.
Durhman, A, K, Rowe, D. B. and Rugh, C. L. (2006). Effect of substrate depth on initial growth, coverage, and survival of 25 succulent green roof plant. Horticultural Science. 42:588–95.
Dehnavardi Movahedi, F. M,. Modares. Sanavi, S., Sorushzadeh , . M. A. and Jalali , M. (2003). Change in total soluble sugar, prolin,. colorophyll and clorophyll florosance safflower variaties under drought stress and zins spraying and manganes . Desert. 9:93-109.
Ellefson, C. L. and Winger. D. (2004). Xeriscape Colorado: the complete guide. Westcliffe Press, 256 pages.
Emilsson, T. (2008). Vegetation development on extensive vegetated green roofs: Influence of substrate composition, establishment method and species mix. Ecological Engineering. 33: 265–277.
Elmongy, M., Zhou, H., Cao, Y., Liu, B. and Xia, Y. (2018). The effect of humic acid on endogenous hormone levels and antioxidant enzyme activity during in vitro rooting of evergreen azalea. Scientia Horticulturae. 227:234-243.
Esringu, A., Sezen, I., Aytali, B. and Ercisli, S. (2015). Effect of humic and fulvic acid application on growth parameters in Impatiens walleriana L. Aka. Ziraat Dergisi. 4(1): 37-42.
Fai Chow, M., Abu Bakar, M.F., Khai Wong, J. and Ling, L. (2021). Evapotranspiration Measurement and Estimation of Crop Coefficient for Native Plant Species of Green Roof in the Tropics. Water. 13: 1669. https://doi.org./10.3390/w13121669.
FLL. (2018). Guidelines for the Planning, Construction and Maintenance of Green Roof. Germany: Landscaping Development Research Society.
Francis, R.A. and Lorimer, J. (2011). Urban reconciliation ecology: the potential of living roofs and walls. J. Environ. Manag. 92: 1429–1437.
Getter, K. L. and Rowe, D.B. (2006). The role of extensive green roofs in sustainable development. Hort Science .41: 1276–1285.
Getter, K.L and Rowe, B.D. (2008). Media depth influences Sedum green roof establishment. Urban Ecosystems. 11(4):361-372.
Ghorbbani, R. (2016). Effect of humic acid pretreatment on germination and growth of marigold under drought stress. Master Thesis. Faculty of Agriculture, Birjand University.
Imran, H.M., Kala, J., Ng, A.W.M. and Muthukumaran, S. (2018). Effectiveness of green and cool roofs in mitigating urban heat island effects during a heatwave event in the city of Melbourne in southeast Australia. Journal of Cleaner Production. 197: 393-405.
Ismail, A. I. M., El-Shafey, O. I., Amr, M. H. A. and El-Maghraby, M. S. (2014). Pumice characteristics and their utilization on the synthesis of mesoporous minerals and on the removal of heavy metals, International Scholarly Research Notices .259-379.
Jiang, H. and Fry, J. (1998). Drought responses of perennial ryegrass treated with plant growth regulators. Horticultural Science. 33: 270–273.
Jim, C.Y. and Tsang, S.W. (2011). Ecological energetics of tropical intensive green roof. Energy and Buildings. 43 (10): 2696–2704.
Jozay, M., Rabbani, M. and Kazemi, F. (2021). The impact of humic acid solutions and types of growing media on some morphophysiological and biochemical features of Syngonium sp. and Pothos sp. plants in interior green wall conditions. Plant Archives. 21(1): 2240-2252.
Kazemi, F., and Mohorkob, R. (2017). Review on the roles and effects of growing media on plant performance in green roofs in world climates. Urban Forestry & Urban Greening. 23: 13-26.
Kazemi, F., Rabbani, M. and Jozay, M. (2020). Investigating the plant and air-quality performances of an internal green wall system under hydroponic conditions. Journal of Environmental Management. 275: 1-10.
Karimi, A., Noshadi, M. and Ahmadzadeh, M. (2008). Effect of application of water-absorbing modifier (Igita) on soil water, plant growth and irrigation, Agricultural Science and Technology and Natural Resources. 46: 414-403.
Kohler, M., Liu, K. and Rowe, B. (2007). Green roofs as urban ecosystems: ecological structures, functions, and services. Bioscience. 57: 823–833.
Lucket, K. ( 2009). Green roof construction and maintenance, The McGraw Hills Company.
Liu, X., Xu, CH., Zhong, Z., Li, Y., Yuan, Z. and Cao, J. (2017). Comparison of 16 models for reference crop evapotranspiration against weighing lysimeter measurement. Agricultural Water Management. 184: 144-145.
Marasco, D. E., Hunter, B. N., Culligan, P. J., Gaffin, S. R. and McGillis, W. R. (2014). Quantifying evapotranspiration from urban green roofs: a comparison of chamber measurements with commonly used predictive methods. Environmental Science & Technology. 48: 10273–10281. http://dx.doi.org/10.1021/es501699h.
McCann, S.T. and Huang, B. ( 2008). Drought responses of kentucky bluegrass and creeping bentgrass as affected by abscisic acid and trinexapac-ethyl. Horticultural Science. 133; 20–26.
Monterusso, M.A., Rowe, D.B. and Rugh, C. L. (2005). Establishment and persistence of Sedum spp. and native taxa for green roof applications Horticultural Science. 40: 391–396.
Mora, V., Bacaicoa, E., Zamarrenõ, A.M., Aguirre, E., Garnica, M., Fuentes, M. and Garcıa-Mina, J. M. (2012). Action of humic acid on promotion of cucumber shoot growth involves nitrate-related changes associated with the root-to-shoot distribution of cytokinins, polyamines and mineral nutrients. Journal of Plant Physiology. 167: 633–642.
Muscolo, A., Sidari, M. and Nardi, S. (2013). Humic substance: Relationship between structure and activity. Deeper information suggests univocal findings. Journal of Geochemical Exploration.129: 57-63.
Naderi, R., Taheri, M. and Refahi, Sh. (2018.) Investigation of yield of some plants in different substrates and planting depth in dry conditions in wide green roof. Horticultural Sciences of Iran. 48 (1): 113-122.
Nagase, A. (2020). Novel application and reused materials for extensive green roof substrates and drainage layers in Japan – Plant growth and moisture uptake implementation. Ecological Engineering. 153:1-10.
Nagase, A. and Dunnett, N. (2012). Amount of water runoff from different vegetation types on extensive green roofs: Effects of plant species, diversity and plant structure. Landscape and Urban Planning. 104(3-4):356-363.
Nardi, S., Pizzeghello, D., Muscolo, A. and Vianello, A. (2002). Physiological effects of humic substances on higher plants. Soil Biology and Biochemistry. 34(11): 1527-1536.
Nikbakht A., Kafi, M., Babalar, M., Xia, PY. Luo, A. and Etemadi, N. (2015). Effect of humic acid on plant growth, nutrient uptake, and postharvest life of gerbera. Journal of Plant Nutrition. 31: 2155–2167.
Oberndorfer E., Lundholm, J., Bass, B., Coffman, R. R., Doshi, H. and Dunnett, N. (2007). Green roofs as urban ecosystems: ecological structures, functions, and services. BioScience. 57:823–33.
Ondo˜no, S., Martínez-Sánchez, J.J. and Moreno, J. L.( 2016). The composition and depthof green roof substrates affect the growth of Silene vulgaris and Lagurus ovatusspecies and the C and N sequestration under two irrigation conditions. Journal of Environmental Management. 166: 330–340.
Qin, M., Walton, G., Belarbi,R. and Allard, F. (2011). Simulation of whole building coupled hygrothermal-airflow transfer in different climates, Energy Conversion and Management. 52: 1470–1478. doi:10.1016/j.enconman.2010.10.010.
Pilehvar, M. (2014). The effect of irrigation regime on survival, visual, thermodynamic and physiophysiological traits of some medicinal plants in the single and multiple green roof system. Master Thesis. Faculty of Agriculture, Ferdowsi University of Mashhad. (In Persian).
Rabbani Kheir Khah, S.M., Kazemi, F. and Shoor, M. (2019). Evaluating the effect of superabsorbents on soil moisture and physiological characteristics of turfgrasses. Desert. 24(2): 229-240.
Razzaghmanesh, M., Beecham, S. and Kazemi, F. )2014(. The growth and survival of plants in urban green roofs in a dry climate. Science of the Total Environment. 476-477:288-297.
Rowe, D. B., Monterusso, M. A. and Rugh, C.L )2006(. Assessment of heat-expanded slate and fertility requirements in green roof substrates. Hort Techno. 16(3): 471–477.
Salman, S. R., Abou-hussein, S.D., Abdel-Mawgoud, A. M. R. and El-Nemr, M.A. )2005(. Fruit yield and quality of watermelon as affected by hybrids and humic acid application. Journal of Applied Sciences Research. 1:51-58.
Samar Raza, M.A., Saleem, M.F., Khan, I.H., Jamil, M., Ijaz, M. and Khan, M.A. (2012). Evaluating the drought stress tolerance efficiency of wheat (triticum aestivum l.) Cultivars. Russian Journal f Agricultural and Socio-Economic Sciences. 12(12): 41-46.
Savi, T., Marin, M., Boldrin, D., Incerti, G., Andr. S. and Nardin, A. )2014(. Green roofs for a drier world: Effects of hydrogel amendment on substrate and plant water status. Science of the Total Environment. 490: 467-476.
Schweitzer, O. and Erell, E. )2014(. Evaluation of the energy performance and irrigation requirements of extensive green roofs in a water-scarce Mediterranean climate. Energy and Buildings. 68: 25-32.
Shafique, M., Kim, R. and Rafiq, M. )2018(. Green roof benefits, opportunities and challenges – A review. Renewable and Sustainable Energy Reviews. 90: 757-773.
Simmons, M. T., Gardiner, Brian, Windhager, Steve, Tinsley, M. and Jeannine, R. )2008(. Green roofs are not created equal: the hydrologic and thermal performance of six different extensive green roofs and reflective and non-reflective roofs in a sub-tropical climate Urban Ecosystems. 11: 339–348. http://dx.doi.org/10.1007/s11252-008-0069-4.
Slinkard K. and Singleton VL. (1977). Total phenol analysis; automation and comparison with manual methods. American Journal of Enology and Viticulture. 28: 49-55.
Susca, T., Gaffin, S.R. and Dell’Osso, G.R. )2011(. Positive effects of vegetation: Urban heat island and green roofs, Environmental Pollution. 159: 2119–2126. doi:10.1016/j.envpol.2011.03.007.
Tahiri, A., Destain, J., Thonart, J.C. and Druart , PH. (2015). In vitro model to study the biological properties of humic fractions from landfill leachate and leonardite during root elongation of Alnus glutinosa L. Gaertn and Betula pendula Roth. Plant Cell, Tissue and Organ Culture. 122: 739–749.
Tan, C.L., Tan, P.Y., Wong, N.H., Takasuna, H., Kudo, T., Takemasa, Y., Lim, C.V.J. and Chua, H.X.V. (2017). Impact of soil and water retention characteristics on green roof thermal performance, Energy Build. 152 (2017) 830–842. doi:10.1016/j.enbuild.2017.01.011
Tattini, M., Bertoni, P., Landi, A. and Traversi, M.L. )1991(. Effect of humic acids on growth and nitrogen uptake of container grown olive plant. Acta Horticulturae. 286: 125-128.
Turkmen, O., Demir, S., Sensoy, S. and Dursun, A. )2005(. Effect of arbuscular mycorrhizal fungus and humi acid on the seedling development and nutrient content of pepper growli nader saline soil conditions. Journal of Biological Sciences. 5: 565-574.
Vahdati, N., Tehranifar, A. and Kazemi, F. )2017(. Assessing chilling and drought tolerance of different plant genera on extensive green roofs in an arid climate region in Iran. Environmental Management . 19: 215-223.
VanWoert, N. D., Rowe, D. B., Andresen, J. A., Rugh, C. L. and Xiao, L. )2005(. Watering regime and green roof susbtrate design affect Sedum plant growth. Horticultural Science. 40(4): 659–64.
Walters., S. and Midden, K. )2018(. Sustainability of Urban Agriculture: Vegetable Production on Green Roofs. Agriculture. 8(168):1-16.
Williams, NSG., Hughes, RE., Jones, NM., Bradbury, D.A. and Rayner, J. P. )2010(. The performance of native and exotic species for extensive green roofs in Melbourne, Australia. Acta Horticulturae.881: 689–96.
Yang, J., Mohan Kumar, D.L., Pyrgou, A., Chong, Santamouris, M., Kolokotsa, D. and Eang Lee, S. )2018(. Green and cool roofs’ urban heat island mitigation potential in tropical climate. Solar Energy.173: 597-609.
Zhoua, L., Monreal, C., Xu, SH., McLaughlin, N., Zhang, H., Hao, G. and Liu, J. )2019(. Effect of bentonite-humic acid application on the improvement of soil structure and maize yield in a sandy soil of a semi-arid region. Geoderma. 338 :269-280.
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Abedini, T., Moradi, P. and Hani, A. (2015). Effect of organic fertilizer and foliar application of humic acid on some quantitative and qualitative yield of Pot marigold. Journal of Novel Applied Sciences.10:1100-1103.
Abedi Kopaei, J. and Sohrab, F. (2004). Effect of zeolite and bentonite minerals on soil hydraulic properties. Proceedings of the 12th Iranian Conference on Crystallography and Mineralogy, Shahid Chamran University, Ahvaz, page 567. (In Persian with English Summary).
Allahdadi, A.B., Ghamsari, M., Akbari, G.A. and Zohourmehr, M. (2005). Study the effect of different levels of super absorbent polymer and irrigation on corn (Zea mays L.) growth and yield. 3th Congress on Super Absorbent Hydrogel Application in Agriculture. Research Center for Polymer and Petrochemical of Iran. (In Persian with English Summary).
Arancon, NQ, Yardin E, Edwards, CA. and Lee, S. (2003). The trophic diversity of nematode communities in soils treated with vermicomposts. Pedobiologia. 47: 731-735.
Arnon, D.S. )1940(. Copper enzyme in isolated chloroplast polyphenol oxidase in Beta Vulgaris. J. Plant Physiol. 24: 1-15.
Barrs, H. D. and Weatherley, P. E. (1962). A reexamination of the relative turgidity technique for the estimating of water deficits in leaves. Australian Journal of Biological Sciences. 15: 413-428.
Baryła, A., Gnatowski, T., Karczmarczyk, A. and Szatylowicz, J. (2019). Changes in temperature and moisture content of an extensive-type green roof. Sustainability. 11: 2498.
Bevilacqua, P., Coma, J., Pérez, G., Chocarro, C., Juárez, A., Solé, C., De Simone, M. and Cabeza, L. F. (2015). Plant cover and floristic composition effect on thermal behaviour of extensive green roofs. Building and Environment. 92: 305–316. doi:10.1016/j.buildenv.2015.04.026.
Blum, A. and Ebercon, A. )1981(. Cell membrane stability as a measure of drought and heat tolerance in wheat. Crop Science. 21:43-47.
Bianchini, F. and Hewage, K. (2012). How green are the green roofs? Lifecycle analysis of green roof materials. Building and Environment. 48:57–65.
Bidgoli, Z. (2016). Investigation of the use of superabsorbent in the amount of water retention on the green roof. Master Thesis. Faculty of Agriculture, University of Zanjan (In Persian).
Cacco, G., Attina, E., Gelsomino, A. and Sidari, M. (2000). Effect of nitrate and humic substances of different molecular size on kietic parameters of nitrate uptake in wheat seedlings. Journal of Plant Nutrition and Soil Science’s. 163: 313-320.
Calderin-Garcia, A., de Souza, L.G.A., Pereira, M.G., Castro, R.N., Garcia-Mina, J.M., Zonta, E., Lisboa, F.J.G. and Berbara, R.L.L. (2016). Structure-property-function relationship in humic substances to explain the biological activity in plants. Scientific Reports.6: 20798.
Canellas, L., Olivares, F., Aguiar, N., Jones, D., Nebbioso, A., Mazzei, P. and Piccolo, A. (2015). Humic and fulvic acids as biostimulants in horticulture. Scientia Horticulturae. 196: 15-27.
Cascone, S. (2019). Green Roof Design: State of the Art on Technology and Materials. Sustainability. 11: 3020, 1-28. doi:10.3390/su11113020.
Dalvand, M. (2016). The effect of humic acid on seed germination, growth indices and drought stress in parsley, Master Thesis, Faculty of Agriculture, Arak University (In Persian).
Deenavarman, M. , Lourdusamy, D. K. , Thangaselvabai T. and Venkatesan, K. (2018). "Effect of different media incorporated with Pusa hydrogel on growth and watering frequency of potted foliage plant, Arrowhead (Syngonium podophyllum Schott.). Journal of Agriculture and Ecology. pp. 71-76.
Durhman, A, K, Rowe, D. B. and Rugh, C. L. (2006). Effect of substrate depth on initial growth, coverage, and survival of 25 succulent green roof plant. Horticultural Science. 42:588–95.
Dehnavardi Movahedi, F. M,. Modares. Sanavi, S., Sorushzadeh , . M. A. and Jalali , M. (2003). Change in total soluble sugar, prolin,. colorophyll and clorophyll florosance safflower variaties under drought stress and zins spraying and manganes . Desert. 9:93-109.
Ellefson, C. L. and Winger. D. (2004). Xeriscape Colorado: the complete guide. Westcliffe Press, 256 pages.
Emilsson, T. (2008). Vegetation development on extensive vegetated green roofs: Influence of substrate composition, establishment method and species mix. Ecological Engineering. 33: 265–277.
Elmongy, M., Zhou, H., Cao, Y., Liu, B. and Xia, Y. (2018). The effect of humic acid on endogenous hormone levels and antioxidant enzyme activity during in vitro rooting of evergreen azalea. Scientia Horticulturae. 227:234-243.
Esringu, A., Sezen, I., Aytali, B. and Ercisli, S. (2015). Effect of humic and fulvic acid application on growth parameters in Impatiens walleriana L. Aka. Ziraat Dergisi. 4(1): 37-42.
Fai Chow, M., Abu Bakar, M.F., Khai Wong, J. and Ling, L. (2021). Evapotranspiration Measurement and Estimation of Crop Coefficient for Native Plant Species of Green Roof in the Tropics. Water. 13: 1669. https://doi.org./10.3390/w13121669.
FLL. (2018). Guidelines for the Planning, Construction and Maintenance of Green Roof. Germany: Landscaping Development Research Society.
Francis, R.A. and Lorimer, J. (2011). Urban reconciliation ecology: the potential of living roofs and walls. J. Environ. Manag. 92: 1429–1437.
Getter, K. L. and Rowe, D.B. (2006). The role of extensive green roofs in sustainable development. Hort Science .41: 1276–1285.
Getter, K.L and Rowe, B.D. (2008). Media depth influences Sedum green roof establishment. Urban Ecosystems. 11(4):361-372.
Ghorbbani, R. (2016). Effect of humic acid pretreatment on germination and growth of marigold under drought stress. Master Thesis. Faculty of Agriculture, Birjand University.
Imran, H.M., Kala, J., Ng, A.W.M. and Muthukumaran, S. (2018). Effectiveness of green and cool roofs in mitigating urban heat island effects during a heatwave event in the city of Melbourne in southeast Australia. Journal of Cleaner Production. 197: 393-405.
Ismail, A. I. M., El-Shafey, O. I., Amr, M. H. A. and El-Maghraby, M. S. (2014). Pumice characteristics and their utilization on the synthesis of mesoporous minerals and on the removal of heavy metals, International Scholarly Research Notices .259-379.
Jiang, H. and Fry, J. (1998). Drought responses of perennial ryegrass treated with plant growth regulators. Horticultural Science. 33: 270–273.
Jim, C.Y. and Tsang, S.W. (2011). Ecological energetics of tropical intensive green roof. Energy and Buildings. 43 (10): 2696–2704.
Jozay, M., Rabbani, M. and Kazemi, F. (2021). The impact of humic acid solutions and types of growing media on some morphophysiological and biochemical features of Syngonium sp. and Pothos sp. plants in interior green wall conditions. Plant Archives. 21(1): 2240-2252.
Kazemi, F., and Mohorkob, R. (2017). Review on the roles and effects of growing media on plant performance in green roofs in world climates. Urban Forestry & Urban Greening. 23: 13-26.
Kazemi, F., Rabbani, M. and Jozay, M. (2020). Investigating the plant and air-quality performances of an internal green wall system under hydroponic conditions. Journal of Environmental Management. 275: 1-10.
Karimi, A., Noshadi, M. and Ahmadzadeh, M. (2008). Effect of application of water-absorbing modifier (Igita) on soil water, plant growth and irrigation, Agricultural Science and Technology and Natural Resources. 46: 414-403.
Kohler, M., Liu, K. and Rowe, B. (2007). Green roofs as urban ecosystems: ecological structures, functions, and services. Bioscience. 57: 823–833.
Lucket, K. ( 2009). Green roof construction and maintenance, The McGraw Hills Company.
Liu, X., Xu, CH., Zhong, Z., Li, Y., Yuan, Z. and Cao, J. (2017). Comparison of 16 models for reference crop evapotranspiration against weighing lysimeter measurement. Agricultural Water Management. 184: 144-145.
Marasco, D. E., Hunter, B. N., Culligan, P. J., Gaffin, S. R. and McGillis, W. R. (2014). Quantifying evapotranspiration from urban green roofs: a comparison of chamber measurements with commonly used predictive methods. Environmental Science & Technology. 48: 10273–10281. http://dx.doi.org/10.1021/es501699h.
McCann, S.T. and Huang, B. ( 2008). Drought responses of kentucky bluegrass and creeping bentgrass as affected by abscisic acid and trinexapac-ethyl. Horticultural Science. 133; 20–26.
Monterusso, M.A., Rowe, D.B. and Rugh, C. L. (2005). Establishment and persistence of Sedum spp. and native taxa for green roof applications Horticultural Science. 40: 391–396.
Mora, V., Bacaicoa, E., Zamarrenõ, A.M., Aguirre, E., Garnica, M., Fuentes, M. and Garcıa-Mina, J. M. (2012). Action of humic acid on promotion of cucumber shoot growth involves nitrate-related changes associated with the root-to-shoot distribution of cytokinins, polyamines and mineral nutrients. Journal of Plant Physiology. 167: 633–642.
Muscolo, A., Sidari, M. and Nardi, S. (2013). Humic substance: Relationship between structure and activity. Deeper information suggests univocal findings. Journal of Geochemical Exploration.129: 57-63.
Naderi, R., Taheri, M. and Refahi, Sh. (2018.) Investigation of yield of some plants in different substrates and planting depth in dry conditions in wide green roof. Horticultural Sciences of Iran. 48 (1): 113-122.
Nagase, A. (2020). Novel application and reused materials for extensive green roof substrates and drainage layers in Japan – Plant growth and moisture uptake implementation. Ecological Engineering. 153:1-10.
Nagase, A. and Dunnett, N. (2012). Amount of water runoff from different vegetation types on extensive green roofs: Effects of plant species, diversity and plant structure. Landscape and Urban Planning. 104(3-4):356-363.
Nardi, S., Pizzeghello, D., Muscolo, A. and Vianello, A. (2002). Physiological effects of humic substances on higher plants. Soil Biology and Biochemistry. 34(11): 1527-1536.
Nikbakht A., Kafi, M., Babalar, M., Xia, PY. Luo, A. and Etemadi, N. (2015). Effect of humic acid on plant growth, nutrient uptake, and postharvest life of gerbera. Journal of Plant Nutrition. 31: 2155–2167.
Oberndorfer E., Lundholm, J., Bass, B., Coffman, R. R., Doshi, H. and Dunnett, N. (2007). Green roofs as urban ecosystems: ecological structures, functions, and services. BioScience. 57:823–33.
Ondo˜no, S., Martínez-Sánchez, J.J. and Moreno, J. L.( 2016). The composition and depthof green roof substrates affect the growth of Silene vulgaris and Lagurus ovatusspecies and the C and N sequestration under two irrigation conditions. Journal of Environmental Management. 166: 330–340.
Qin, M., Walton, G., Belarbi,R. and Allard, F. (2011). Simulation of whole building coupled hygrothermal-airflow transfer in different climates, Energy Conversion and Management. 52: 1470–1478. doi:10.1016/j.enconman.2010.10.010.
Pilehvar, M. (2014). The effect of irrigation regime on survival, visual, thermodynamic and physiophysiological traits of some medicinal plants in the single and multiple green roof system. Master Thesis. Faculty of Agriculture, Ferdowsi University of Mashhad. (In Persian).
Rabbani Kheir Khah, S.M., Kazemi, F. and Shoor, M. (2019). Evaluating the effect of superabsorbents on soil moisture and physiological characteristics of turfgrasses. Desert. 24(2): 229-240.
Razzaghmanesh, M., Beecham, S. and Kazemi, F. )2014(. The growth and survival of plants in urban green roofs in a dry climate. Science of the Total Environment. 476-477:288-297.
Rowe, D. B., Monterusso, M. A. and Rugh, C.L )2006(. Assessment of heat-expanded slate and fertility requirements in green roof substrates. Hort Techno. 16(3): 471–477.
Salman, S. R., Abou-hussein, S.D., Abdel-Mawgoud, A. M. R. and El-Nemr, M.A. )2005(. Fruit yield and quality of watermelon as affected by hybrids and humic acid application. Journal of Applied Sciences Research. 1:51-58.
Samar Raza, M.A., Saleem, M.F., Khan, I.H., Jamil, M., Ijaz, M. and Khan, M.A. (2012). Evaluating the drought stress tolerance efficiency of wheat (triticum aestivum l.) Cultivars. Russian Journal f Agricultural and Socio-Economic Sciences. 12(12): 41-46.
Savi, T., Marin, M., Boldrin, D., Incerti, G., Andr. S. and Nardin, A. )2014(. Green roofs for a drier world: Effects of hydrogel amendment on substrate and plant water status. Science of the Total Environment. 490: 467-476.
Schweitzer, O. and Erell, E. )2014(. Evaluation of the energy performance and irrigation requirements of extensive green roofs in a water-scarce Mediterranean climate. Energy and Buildings. 68: 25-32.
Shafique, M., Kim, R. and Rafiq, M. )2018(. Green roof benefits, opportunities and challenges – A review. Renewable and Sustainable Energy Reviews. 90: 757-773.
Simmons, M. T., Gardiner, Brian, Windhager, Steve, Tinsley, M. and Jeannine, R. )2008(. Green roofs are not created equal: the hydrologic and thermal performance of six different extensive green roofs and reflective and non-reflective roofs in a sub-tropical climate Urban Ecosystems. 11: 339–348. http://dx.doi.org/10.1007/s11252-008-0069-4.
Slinkard K. and Singleton VL. (1977). Total phenol analysis; automation and comparison with manual methods. American Journal of Enology and Viticulture. 28: 49-55.
Susca, T., Gaffin, S.R. and Dell’Osso, G.R. )2011(. Positive effects of vegetation: Urban heat island and green roofs, Environmental Pollution. 159: 2119–2126. doi:10.1016/j.envpol.2011.03.007.
Tahiri, A., Destain, J., Thonart, J.C. and Druart , PH. (2015). In vitro model to study the biological properties of humic fractions from landfill leachate and leonardite during root elongation of Alnus glutinosa L. Gaertn and Betula pendula Roth. Plant Cell, Tissue and Organ Culture. 122: 739–749.
Tan, C.L., Tan, P.Y., Wong, N.H., Takasuna, H., Kudo, T., Takemasa, Y., Lim, C.V.J. and Chua, H.X.V. (2017). Impact of soil and water retention characteristics on green roof thermal performance, Energy Build. 152 (2017) 830–842. doi:10.1016/j.enbuild.2017.01.011
Tattini, M., Bertoni, P., Landi, A. and Traversi, M.L. )1991(. Effect of humic acids on growth and nitrogen uptake of container grown olive plant. Acta Horticulturae. 286: 125-128.
Turkmen, O., Demir, S., Sensoy, S. and Dursun, A. )2005(. Effect of arbuscular mycorrhizal fungus and humi acid on the seedling development and nutrient content of pepper growli nader saline soil conditions. Journal of Biological Sciences. 5: 565-574.
Vahdati, N., Tehranifar, A. and Kazemi, F. )2017(. Assessing chilling and drought tolerance of different plant genera on extensive green roofs in an arid climate region in Iran. Environmental Management . 19: 215-223.
VanWoert, N. D., Rowe, D. B., Andresen, J. A., Rugh, C. L. and Xiao, L. )2005(. Watering regime and green roof susbtrate design affect Sedum plant growth. Horticultural Science. 40(4): 659–64.
Walters., S. and Midden, K. )2018(. Sustainability of Urban Agriculture: Vegetable Production on Green Roofs. Agriculture. 8(168):1-16.
Williams, NSG., Hughes, RE., Jones, NM., Bradbury, D.A. and Rayner, J. P. )2010(. The performance of native and exotic species for extensive green roofs in Melbourne, Australia. Acta Horticulturae.881: 689–96.
Yang, J., Mohan Kumar, D.L., Pyrgou, A., Chong, Santamouris, M., Kolokotsa, D. and Eang Lee, S. )2018(. Green and cool roofs’ urban heat island mitigation potential in tropical climate. Solar Energy.173: 597-609.
Zhoua, L., Monreal, C., Xu, SH., McLaughlin, N., Zhang, H., Hao, G. and Liu, J. )2019(. Effect of bentonite-humic acid application on the improvement of soil structure and maize yield in a sandy soil of a semi-arid region. Geoderma. 338 :269-280.
