اثر ضد باکتریایی و ضد بیوفیلمی اسانس آویشن شیرازی (Zataria multiflora) بر سالمونلاهای جداشده از طیور و انسان به روش حداقل غلظت مهارکنندگی رشد
محورهای موضوعی : آسیب شناسی درمانگاهی دامپزشکی
زینال زینالی
1
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جلال شایق
2
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شاهین تفنگدارزاده
3
1 - دانش‌آموخته دکترای حرفه‌ای دامپزشکی، دانشکده دامپزشکی، واحد شبستر، دانشگاه آزاد اسلامی، شبستر، ایران.
2 - دانشيار گروه دامپزشکی، دانشکده دامپزشکی، واحد شبستر، دانشگاه آزاد اسلامی، شبستر، ایران.
3 - استادیار گروه علوم پایه، دانشکده علوم پایه، واحد شبستر، دانشگاه آزاد اسلامی، شبستر، ایران.
کلید واژه: گیاهان دارویی, آویشن شیرازی, سالمونلا, بیوفیلم, روش حداقل غلظت مهارکنندگی رشد.,
چکیده مقاله :
اسانس‌های گیاهی، ترکیبات پیچیده‌ای از اجزای مختلف شیمیایی با مقادیر گوناگون می‌باشند. این مواد یکی از منابع بالقوه واجد ترکیبات ضدباکتریایی هستند و برای این منظور بسیار موثر و مفید می‌باشند. هدف از این مطالعه، بررسی اثر ضدباکتریایی اسانس آویشن شیرازی بر سالمونلاهای جداشده از طیور و انسان به¬روش حداقل غلظت مهارکنندگی رشد (Minimum Inhibitory Concentration; MIC) بود. بدین ¬منظور، برای استخراج اسانس از روش تقطیر با بخار آب استفاده شد، سپس با استفاده از روش کروماتوگرافی گازی-طیف جرمی (Gas chromatography-Mass Spectroscopy; Gc-Ms) ترکیب اسانس مشخص گردید. برای تعیین MIC اسانس آویشن شیرازی از روش براث میکرودایلوشن و برای بررسی اثر ضد بیوفیلمی اسانس آویشن شیرازی، از تکنیک الایزا استفاده گردید. طبق نتایج، بیش از 9/64 درصد اسانس از سه ماده تیمول، کاراکول و لینانول به ترتیب با 62/46، 85/13 و 95/8 درصد تشکیل شده بود. مشخص شد که میزان MIC برای اسانس آویشن شیرازی در جدایه‌های سالمونلا در محدوده 5/12-39/0 میلی‌گرم بر میلی‌لیتر بود. همچنین، میزان حداقل غلظت کشندگی (Minimum Bactericidal Concentration; MBC) اسانس آویشن شیرازی (Zataria multiflora) در جدایه‌های سالمونلا در محدوده 5/12-56/1 میلی¬گرم بر میلی‌لیتر بود. قرارگیری جدایه‌های باکتری سالمونلا با آویشن شیرازی در سطح احتمالی یک درصد به طور معنی‌داری (001/0>p) باعث کاهش تولید بیوفیلم شد. نتایج مطالعه نشان داد که اسانس آویشن شیرازی دارای اثرات ضدباکتریایی قابل‌توجهی می‌باشد و بیوفیلم جدایه‌های باکتری سالمونلا را تا میزان قابل توجهی کاهش می¬دهد.
Plant essential oils are complex compounds of different chemical components with different amounts. These substances are one of the potential sources of antibacterial compounds and are very effective and useful for this purpose. The aim of this study was to investigate the antibacterial effect of Zataria multiflora essential oil on Salmonella isolated from poultry and humans using the Minimum Inhibitory Concentration (MIC) method. Steam distillation was used to extract the essential oil, and then the composition of the essential oil was determined using the Gas Chromatography-Mass Spectroscopy (Gc-Ms) method. Broth microdilution method was used to determine the MIC of Zataria multiflora essential oil. Also, ELISA was used to investigate the anti-biofilm effect of Zataria multiflora essential oil. Based on the results obtained in the present study, it was shown that more than 64.9% of essential oil consists of three substances: thymol, caracol, and linanol which make up46.62%, 13.85%, and 8.95% of the essential oil repectively. It was found that the MIC level For Zataria multiflora essential oil was in the range of 0.39-12.5 mg/ml in Salmonella isolates. Also, based on the results obtained in this study, the minimum bactericidal concentration (MBC) of applied essential oil in Salmonella isolates was in the range of 1.56-12.5 mg/ml. The placement of Salmonella bacteria isolates alongside essential oil at a possible level of 1% significantly (p<0.001) decreased biofilm production. Overall, the results of this research showed that Shirazi thyme essential oil has significant antibacterial effects and reduced biofilm production of Salmonella bacteria isolates by a significant percentage.
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