Antibacterial and antibiofilm effects of Shirazi thyme (Zataria multifora) essential oil on Salmonella isolates from poultry and humans using the Minimum Inhibitory Concentration method

Subject Areas : Veterinary Clinical Pathology

zeynal zeynali ¹ , Jalal Shayegh ² , Shahin Tofangdarzadeh ³

1 - D.V.M. Graduate, Faculty of Veterinary Medicine, Islamic Azad University, Shabestar Branch, Shabestar, Iran.
2 - Associate Professor, Department of Veterinary, Faculty of Veterinary Medicine, Islamic Azad University, Shabestar Branch, Shabestar, Iran.
3 - Assistant Professor, Faculty of Basic Science, Islamic Azad University, Shabestar Branch, Shabestar, Iran.

Received: 2023-08-29 Accepted : 2024-06-18 Published : 2023-12-22

Keywords: Biofilm, Medicinal plants, Minimum Inhibitory Concentration Method, Salmonella Zataria multiflora. ,

Abstract :

Plant essential oils are complex compounds of different chemical components with different amounts. These substances are one of the potential sources of antibacterial compounds and are very effective and useful for this purpose. The aim of this study was to investigate the antibacterial effect of Zataria multiflora essential oil on Salmonella isolated from poultry and humans using the Minimum Inhibitory Concentration (MIC) method. Steam distillation was used to extract the essential oil, and then the composition of the essential oil was determined using the Gas Chromatography-Mass Spectroscopy (Gc-Ms) method. Broth microdilution method was used to determine the MIC of Zataria multiflora essential oil. Also, ELISA was used to investigate the anti-biofilm effect of Zataria multiflora essential oil. Based on the results obtained in the present study, it was shown that more than 64.9% of essential oil consists of three substances: thymol, caracol, and linanol which make up46.62%, 13.85%, and 8.95% of the essential oil repectively. It was found that the MIC level For Zataria multiflora essential oil was in the range of 0.39-12.5 mg/ml in Salmonella isolates. Also, based on the results obtained in this study, the minimum bactericidal concentration (MBC) of applied essential oil in Salmonella isolates was in the range of 1.56-12.5 mg/ml. The placement of Salmonella bacteria isolates alongside essential oil at a possible level of 1% significantly (p<0.001) decreased biofilm production. Overall, the results of this research showed that Shirazi thyme essential oil has significant antibacterial effects and reduced biofilm production of Salmonella bacteria isolates by a significant percentage.

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