شناسایی تقلبات موجود در سوسیس و کالباس تهیه شده از گوشت گاو بر اساس شناسایی ژن‌های میتوکندریایی گونه‌های حیوانی در استان تهران

محورهای موضوعی : علوم و صنایع غذایی

سید ابراهیم حسینی ¹ (دانشگاه آزاد اسلامی، واحد علوم و تحقیقات تهران، دانشکده کشاورزی و منابع طبیعی، استادیار گروه علوم و صنایع غذایی، تهران، ایران.)
فرزانه تفویضی ² (- دانشگاه آزاد اسلامی، واحد پرند، استادیار گروه زیست شناسی، پرند، ایران)
مریم تاج آبادی ابراهیمی ³ (دانشگاه آزاد اسلامی، واحد تهرا ن مرکزی، استادیار گروه زیست شناسی، تهران، ایران.)
انوشه شریفان ⁴ (- دانشگاه آزاد اسلامی، واحد علوم و تحقیقات تهران، دانشکده علوم و مهندسی صنایع غذایی، استادیار گروه علوم و صنایع غذایی، تهران، ایران.)

تاریخ دریافت : 1392/05/16 تاریخ پذیرش : 1393/02/31 تاریخ انتشار : 1393/03/01

کلید واژه: سوسیس, شناسایی تقلبات‏, ‏ PCR,

چکیده مقاله :

هدف از این مطالعه توسعه روش PCR‎ خاص گونه‌ها برای شناسایی دقیق و سریع گوشت گونه‌های حیوانی در فرآورده‌های گوشتی برای تشخیص تقلبات احتمالی در این محصولات است. برای تعیین گوشت گاو و مرغ، پرایمرها بر اساس ژن سیتوکروم bمیتوکندریایی طراحی و قطعات bp274 وbp 183به ترتیب برای گوشت گاو و مرغ تکثیر گردید. از ده برند مختلف سوسیس تهیه شده از گوشت گاو در سرتاسر استان تهران نمونه‌گیری گردید. نتایج PCR‎ اختصاصی نشان داد، تمام نمونه‌ها برخلاف بر چسب‌شان حاوی آلایشمرغی بودند و احشای گاوی در پنج نمونه تشخیص داده شد. به‌علاوه، روش PCR‎ قادر است بدون واکنش متقاطع گونه‌های گوشتی را شناسایی کند. این تکنیک به علت سرعت، سادگی، حساسیت و اختصاصی بودن،پتانسیل بالایی برای تشخیص تقلبات گوشتی در سوسیس دارد.

چکیده انگلیسی:

   The aim of this study was to develop a species-specific PCR method for rapid identification of meat species in order to detect any potential fraud in meat products. For cattle and poultry, a set of primers were designed based on the mitochondrial cytochrome b and subsequently a 183 bp as well as 274 bp fragments were amplified for poultry and beef samples, respectively. For the study, ten different brands of beef sausage were obtained across Tehran province. Results of PCR assay demonstrated that unlike their labels, all of the samples were contained poultry residuals‎. Moreover, beef offal was detected only in five samples‎. Since no cross-reaction was observed among the different samples, PCR could be considered as a sensitive and ‎specific method for the identification of meat varieties. In addition, high-speed operation and easiness of the technique are the other advantages of PCR method. Therefore, this technique has the capability for sensitive and ‎specific detection of meat adulteration in sausages. 

منابع و مأخذ:

 

• Arslan, A., Irfan, O.I. and Mehmet, C. (2006). Effect of method of ‎cooking on identification ‎of ‎heat processed beef using polymerase chain ‎reaction (PCR) technique. Meat Science, 72: ‎‎326–330.‎


• Bottero, M.T., Dalmasso, A., Nucera, D., Turi, R.M., Rosati, S., Squadrone, S., Goria, M. and Civera, ‎T. (2003). Development of a PCR assay for the detection of animal tissues in ruminant feeds. Journal ‎of ‎Food Protection, 66: 2307–2312.‎


• Calvo, J.H., Zaragoza, P. and Osta, R. (2001). A quick and more sensitive method to identify pork ‎in ‎processed and unprocessed food by PCR amplification of a new specific DNA fragment. Journal ‎of ‎American Society of Animal Science, 79: 2108–2112.‎


• Che Man, Y.B., Aida, A.A., Raha, A.R. and Son, R. (2007). Identification of pork derivatives ‎in ‎food ‎products by species-specific polymerase chain reaction (PCR) for halal verification. Food ‎Control, ‎‎18: ‎‎885–889.‎


• Chikuni, K., Tabata, T., Saito, M. and Monma, M. (1994). Sequencing of mitochondrial cytochrome ‎‎b genes for the identification of meat species. Animal Science and Technology, 65: 571–579.‎


• Craig, A., Ritchie, A.H. and Mackie, I.M. (1995). Determining the authenticity of raw reformed ‎breaded ‎scampi (Nephrops norvegicus) by electrophoretic techniques. Food Chemistry, 52: 451–454.‎


• Dalmasso, A., Fontanella, E., Piatti, P., Civera, T., Rosati, S. and Bottero, M. (2004). A multiplex PCR ‎assay ‎for the identification of animal species in feedstuffs. Molecular and Cellular Probes, 18: 81–87.‎


• Desjardins, P. and Morais, R. (1999). Sequence and gene organization of the chicken mitochondrial ‎‎‎genome. Journal of Molecular Biology, 212: 599–634.‎


• Doosti, A., Ghasemi Dehkordi, P. and Rahimi, E. (2014). Molecular assay to fraud identification of ‎meat ‎products. Journal of Food Science and Technology, 51(1): 148-152.


• Ebbehoj, K.F. and Thomsen, P.D. (1991a). Species differentiation of heated meat products by ‎DNA ‎hybridization. Meat Science, 30: 221–234.‎


• Ebbehoj, K.F. and Thomsen, P.D. (1991b). Differentiation of closely related species by ‎DNA ‎hybridization. Meat Science, 30: 359–366.‎


• Ergun, S. and Fatih, M.A. (2012). The development of duplex real-time PCR based on ‎SYBR ‎Green florescence for rapid identification of ruminant and poultry origins in foodstuff. ‎Food ‎Chemistry, 130: 1050–1054.‎


• Fajardo, V., GonzaLez, I., Lopez-Calleja, I., Martian, I., Hernandez, P.E., Garciaa, T.R. and Martian, ‎R. ‎‎(2006). PCR–RFLP authentication of meats from red deer (Cervus elaphus), fallow deer (Dama ‎dama), ‎roe deer (Capreolus capreolus), cattle (Bos taurus), sheep (Ovis aries), and goat (Capra hircus). Agricultural and Food Chemistry, 54: 1144–1150.‎


• Ghovvati, S., Nassiri, M.R., Mirhoseini, S.Z., Heravi Moussavi, A. and ‎Javadmanesh, A. (2008). ‎Fraud ‎identification in industrial meat products ‎by ‎multiplex PCR assay. ‎Food ‎Control, 20(8): 696–‎‎‎699. ‎


• Girish, P.S., Anjaneyulu, A.S.R., Viswas, K.N., Anand, M., ‎Rajkumarb, N., Shivakumar B.M. and ‎Bhaskar, S. (2003). Sequence analysis ‎of ‎mitochondrial 12S rRNA gene can identify meat ‎species. ‎Meat ‎Science, ‎‎66: 551–556.‎


• Hunt, D.J., Parkes, H.C. and Lumley, I.D. (1997). Identification of the species of origin of raw ‎and ‎cooked meat products using oligonucleotide probes. Food Chemistry, 60: 437–442.‎ ‎


• Kesmen, Z., Sahin, F. and Yetim, H. (2007). PCR assay for the ‎identification ‎of ‎animal species in ‎cooked ‎sausages. Meat ‎Science, 77: 649–653.‎


• King, N.L. and Kurth, L. (1982). Analysis of raw beef samples for adulterant meat species by ‎enzyme ‎staining of isoelectric focusing gels. Food Science, 47: 1608–1612.‎ ‎


• Leighton Jones, J. (1991). DNA probes: applications in the food industry. Trends in Food Science and ‎Technology, 2: 28–32.‎


• Mane, B.G., Mendiratta, S.K. and Tiwari, A.K. (2009). PCR assay for specific identification of ‎chicken ‎species in meat and meat products. Food Chemistry, 116: 806–810.‎


• Mane, B.G., Mendiratta, S.K. and Tiwari, A.K. (2012). Detection of ‎adulteration ‎of meat and meat products ‎with buffalo meat employing polymerase chain reaction assay, Food Analytical Methods, ‎‎5: 296–‎‎‎300.


• Mafra, I., Ferreira, P.L.V.O., Beatriz, M. and Oliveira, P.P. (2008). ‎Food ‎authentication by PCR-based methods. European Food Research and Technology, 227: 649–665. ‎


• Matsunga, T., Chikuni, K., Tanabe, R., Muroya, S., Shibata, K., Yamada, J. and Shinmura, Y. (1999). A quick ‎and simple method for the identification of meat species and meat products by PCR assay. Meat ‎Science, 51: 143–148.‎


• Meyer, R. and Candrian, U. (1996). PCR-based DNA analysis for the identification and characterization of ‎food components. Lebensmittel-Wissenschaft und Technologie, 29: 1–9.


• Meyer, R., Hoefelein, C., Luethy, J. and Candrian, U. (1995). Polymerase chain reaction–restriction ‎fragment length ‎polymorphism analysis: A simple method for species identification in food. ‎Journal of AOAC International, 78: ‎‎1542–1551‎.


• Pattersan, R.L.S. (1985). Biochemical identification of meat ‎species. ‎Elsevier Applied ‎Science ‎Publishers, London, pp‎. 313–315.


• Rehbein, H., Kress, G. and Schmidt, T. (1997). Application of PCR SSCP to species identification of fishery ‎products. Journal of the Science of Food and Agriculture, 74: 35–41.‎


• Sambrook, J., Fritch, E.F. and Maniatis, T. (1989). Molecular cloning: A ‎laboratory manual. 2th ‎Edition, ‎ Cold spring Habor ‎Laboratory, New York, pp. 8-113‎‏.‏


• Sawyer, J., Wood, C., Shanahan, D., Gout, S. and McDowell, D. (2003). Real-time PCR for ‎quantitative ‎meat species testing. Food Control, 14: 579–583.‎


• Schonherr, J. (2002). Analysis of products of animal origin in feeds by determination of carnosine and ‎related dipeptides by high-performance liquid chromatography. Journal of Agricultural and Food ‎Chemistry, 50: 1945–1950.‎


• Soares, S., Amarala, J.S., Oliveira, M.B.P.P. and Mafra, I. (2013). A SYBR Green real-time ‎PCR assay to detect and quantify pork meat in processed poultry meat products. Meat Science, 94(1): 115-‎‎120


• Umetsu, K. and Yuasa, I. (2005). Recent progress in mitochondrial DNA analysis. ‎Legal ‎Medicine, 7: ‎‎259–262.‎


• Yusop, M.H.M., Mustafa, S., Che-Man, Y.B., Omar, A.R. and Mokhtar, N.F.K. (2012).‎ Detection of ‎raw ‎pork targeting porcine-specific mitochondrial cytochrome B gene by molecular beacon probe real-‎time ‎polymerase chain reaction. Food Analytical Methods, 5: 422–429‎.‎