مطالعه میزان باقی‌مانده انروفلوکساسین در عضلات و کبد ماهیان قزل آلای رنگین کمان در استان چهار محال و بختیاری به روش الایزا

محورهای موضوعی : علوم و صنایع غذایی

فیروز فدایی فرد ¹ (دانشگاه آزاد اسلامی، واحد شهرکرد، دانشکده دامپزشکی، استادیار گروه بهداشت و بیماری‌های آبزیان، شهرکرد، ایران)

تاریخ دریافت : 1391/03/03 تاریخ پذیرش : 1391/08/28 تاریخ انتشار : 1391/03/01

کلید واژه: الایزا, چهار محال و بختیاری, انروفلوکساسین, قزل‌آلای رنگین کمان,

چکیده مقاله :

انروفلوکساسین از آنتی بیوتیک های پرمصرف دامپزشکی است که در سال های اخیر استفاده آن در کنترل بیماری های عفونی ماهیان پرورشی رشد چشمگیری داشته است. هدف از انجام مطالعه حاضر اندازه گیری میزان باقی مانده انروفلوکساسین در عضلات و کبد ماهی قزل آلای رنگین کمان پرورشی استان چهار محال و بختیاری به روش الایزا بود. برای این منظور در فصول بهار و تابستان 1390 به مزارع پرورشی مناطق اردل و کوهرنگ مراجعه و از هر منطقه سه مزرعه انتخاب گردید و 15 نمونه در سه گروه وزنی کمتر از 50 گرم، 50 تا 150 گرم و بیشتر از 150 گرم (5 نمونه از هر گروه وزنی) صورت گرفت. طبق نتایج مطالعه، بیشترین و کمترین درصد باقی مانده انروفلوکساسین به ترتیب در گروه های وزنی زیر 50 گرم و بالای 150 گرم و به میزان 33/58 و 33/23 درصد ردیابی شد. در مقایسه بین مناطق مختلف نیز کوهرنگ با میزان50/11 ± 84/13 و 06/18 ± 95/19 و اردل با میزان 62/11 ± 83/12 و 09/11 ± 22/11 میکروگرم در کیلوگرم آنتی بیوتیک در عضله و کبد به ترتیب دارای بیشترین و کمترین میزان باقی مانده بودند. همچنین میزان باقی مانده در عضله و کبد در فصل بهار 00/13 ± 27/14 و 69/21 ± 06/22 و در فصل تابستان 29/10 ± 40/12 و 31/7 ± 86/8 میکروگرم در کیلوگرم بوده است. در ضمن در تمامی گروه های وزنی میزان باقیمانده انروفلوکساسین در نمونه های کبد بالاتر از عضلات تشخیص داده شد. اما در کل میزان باقی مانده انروفلوکساسین در نمونه های آزمایش شده پایین تر از حد مجاز اتحادیه اروپا بود.

چکیده انگلیسی:

Enrofloxacin is one of the broad-spectrum antibiotics in veterinary medicine which in recent years its application has grown considerably for the control of infectious diseases in farmed fish. Alpha The aim of this study was to quantify enrofloxacin residue in muscle and liver of rainbow trout cultured in Chaharmahal-va-Bakhtiary province using ELISA method. For this, the samples were obtained from the farms located in Ardal and Koohrang areas during the spring and summer of 2011. Three farms were chosen from each area and 15 samples were taken from three weight categories including < 50, 50-150 and > 150 g (5 samples from each category). Results revealed that maximum (58.33) and minimum (23.33) percentage of enrofloxacin residue was determined in <50 g and >150 g categories, respectively. In Koohrang region, the quantity of enrofloxacin residues in muscle and liver samples was 13.84 ± 11.50 and 19.95 ± 18.06, respectively. Meanwhile in Ardal, the quantity of the residue was estimated at 12.83 ± 11.62 and 11.22 ± 11.09, respectively. Considering the sampling time, among spring samples the quantity of the residues in muscle and liver was estimated at 14.27 ± 13 and 22.06 ± 21.69, respectively. The quantity of enrofloxacin among the summer samples was 12.40 ± 10.29 and 8.86 ± 7.31, respectively. Moreover, mean value of enrofloxacin residue in the muscle and liver of Koohrang was higher than Ardal region. It was concluded that enrofloxacin residue was lower than the maximum acceptance limit determined by the European Union.

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