مطالعه شیوع فصلی و جغرافیایی توکسوپلاسما گوندی در شیر نشخوارکنندگان به‌روش nested-PCR

محورهای موضوعی : علوم و صنایع غذایی

مهتاب علیپور عمروآبادی ¹ (دانش آموخته دکترای تخصصی بهداشت مواد غذایی، گروه بهداشت مواد غذایی، واحد شهرکر د، دانشگاه آزاد اسلامی، شهرکرد، ایران)
ابراهیم رحیمی ² (استاد گروه بهداشت مواد غذایی، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران)
امیر شاکریان ³ (استاد مرکز تحقیقات تغذیه و محصولات ارگانیک، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران)

تاریخ دریافت : 1397/11/01 تاریخ پذیرش : 1399/04/02 تاریخ انتشار : 1400/01/01

کلید واژه: شیر خام, Nested-PCR, توکسوپلاسما گوندی, شیوع فصلی,

چکیده مقاله :

توکسوپلاسموزیس یکی از خطرناک ترین بیماری های تک‌یاخته‌ای مشترک بین انسان و دام است که انسان از طرق مختلف به آن آلوده می‌شود. تماس با حیوان آلوده، مصرف مواد غذایی آلوده و انتقال از مادر به جنین از مهم ترین راه های انتقال می‌باشد.مطالعه حاضر اولین گزارش شیوع توکسوپلاسما گوندی در شیر خام گاومیش و شتر در برخی از مناطق ایران می باشد. به این منظور تعداد 440 نمونه شیر خام 5 گونه دام طی چهار فصل سال از استان های اصفهان، چهارمحال و بختیاری، خوزستان و فارس جمع‌آوری و از نظر حضور توکسوپلاسما گوندی به‌روش واکنش nested-PCR مورد مطالعه قرار گرفت. نتایج این مطالعه نشان داد که 26 نمونه (9/5 درصد) آلوده به توکسوپلاسماگوندی بود. بالاترین میزان آلودگی در شیر گوسفند (8 درصد) و پایین ترین آن در شیر گاومیش (28/4 درصد) مشاهده شد. ازنظر شیوع فصلی میزان آلودگی در فصل زمستان (43/11 درصد)، در پاییز (8/6 درصد) و در بهار (03/5 درصد) بود، در حالی‌که هیچ‌یک از نمونه های اخذ شده در تابستان به این انگل آلوده نبود. آلودگی شیر خام و فرآورده های لبنی سنتی به توکسوپلاسما گوندی می تواند به شکل مستقیم از حیوانات آلوده و یا درنتیجه عدم رعایت اصول بهداشتی در طول فرایند شیردوشی، حمل‌ونقل، نگهداری و آلودگی متقاطع باشد. لذا سالم‌سازی مناسب شیر قبل از مصرف پیشنهاد می گردد.TRANSLATE with xEnglishArabicHebrewPolishBulgarianHindiPortugueseCatalanHmong DawRomanianChinese SimplifiedHungarianRussianChinese TraditionalIndonesianSlovakCzechItalianSlovenianDanishJapaneseSpanishDutchKlingonSwedishEnglishKoreanThaiEstonianLatvianTurkishFinnishLithuanianUkrainianFrenchMalayUrduGermanMalteseVietnameseGreekNorwegianWelshHaitian CreolePersian// TRANSLATE with COPY THE URL BELOW BackEMBED THE SNIPPET BELOW IN YOUR SITE Enable collaborative features and customize widget: Bing Webmaster PortalBack//TRANSLATE with xEnglishArabicHebrewPolishBulgarianHindiPortugueseCatalanHmong DawRomanianChinese SimplifiedHungarianRussianChinese TraditionalIndonesianSlovakCzechItalianSlovenianDanishJapaneseSpanishDutchKlingonSwedishEnglishKoreanThaiEstonianLatvianTurkishFinnishLithuanianUkrainianFrenchMalayUrduGermanMalteseVietnameseGreekNorwegianWelshHaitian CreolePersian// TRANSLATE with COPY THE URL BELOW BackEMBED THE SNIPPET BELOW IN YOUR SITE Enable collaborative features and customize widget: Bing Webmaster PortalBack//

چکیده انگلیسی:

Toxoplasmosis is one of the most dangerous common protozoan diseases between humans and animals that infect humans in various ways. Contact with the infected animal, consuming contaminated food, and transmitting from mother to fetus is the most important means of transmission. Based on the recorded data, this study is the first report of Toxoplasma gondii in buffalo and camel milk in some parts of Iran. For this purpose, 440 raw milk samples of 5 species of animals were collected from Isfahan, Chaharmahal & Bakhtiari, Khuzestan, and Fars provinces during four seasons and studied for the presence of Toxoplasma gondii by nested-PCR. The results showed that 26 (5.9%) of the samples were infected with Toxoplasma gondii. The highest infection was observed in sheep milk (8%) and the lowest in buffalo milk (4.28%). The seasonal prevalence of infection was in winter (11.43%), autumn (6.8%), and spring (5.03%), while none of the samples obtained in summer the parasite was not infected. Contamination of raw milk and traditional dairy products with Toxoplasma gondii can either occur directly from infected animals or as a result of non-compliance with the principles of hygiene during the process of milking, transportation, storage, and cross-contamination. Therefore, proper pre-consumption of milk is recommended. TRANSLATE with x English Arabic Hebrew Polish Bulgarian Hindi Portuguese Catalan Hmong Daw Romanian Chinese Simplified Hungarian Russian Chinese Traditional Indonesian Slovak Czech Italian Slovenian Danish Japanese Spanish Dutch Klingon Swedish English Korean Thai Estonian Latvian Turkish Finnish Lithuanian Ukrainian French Malay Urdu German Maltese Vietnamese Greek Norwegian Welsh Haitian Creole Persian   //   TRANSLATE with COPY THE URL BELOW Back EMBED THE SNIPPET BELOW IN YOUR SITE Enable collaborative features and customize widget: Bing Webmaster Portal Back //TRANSLATE with xEnglishArabicHebrewPolishBulgarianHindiPortugueseCatalanHmong DawRomanianChinese SimplifiedHungarianRussianChinese TraditionalIndonesianSlovakCzechItalianSlovenianDanishJapaneseSpanishDutchKlingonSwedishEnglishKoreanThaiEstonianLatvianTurkishFinnishLithuanianUkrainianFrenchMalayUrduGermanMalteseVietnameseGreekNorwegianWelshHaitian CreolePersian //  TRANSLATE with COPY THE URL BELOW BackEMBED THE SNIPPET BELOW IN YOUR SITE Enable collaborative features and customize widget: Bing Webmaster PortalBack//

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