بررسی اثر محلول‌پاشی اسیدهای آمینه‌ آلانین، پرولین و گلوتامین بر برخی خصوصیات مورفوفیزیولوژیک و آنزیمی گیاه گشنیز (sativum L. Coriandrum)

محورهای موضوعی : فصلنامه کیفیت و ماندگاری تولیدات کشاورزی و مواد غذایی

الهام دانائی ¹ , محمدعلی شریف زاده احتشامی ²

1 - استادیار، گروه علوم باغبانی، واحد گرمسار، دانشگاه آزاد اسلامی، گرمسار، ایران
2 - دانشجو کارشناسی ارشد، گروه علوم باغبانی، واحد گرمسار، دانشگاه آزاد اسلامی، گرمسار، ایران

تاریخ دریافت : 1402/02/25 تاریخ پذیرش : 1402/03/06 تاریخ انتشار : 1402/06/21

کلید واژه: گشنیز, پروتئین, ازت, آنزیم,

چکیده مقاله :

اسیدهای آمینه محرک‌های زیستی هستند که کاربرد آن‌ها در گیاهان نقش موثری بر بهبود متابولیسم، رشد و عملکرد گیاهان دارد که در این پژوهش تاثیر اسیدهای آمینه ‌آلانین، پرولین و گلوتامین بر برخی ویژگی‌های رشدی، فیزیولوژیک و آنزیمی گیاه گشنیز (sativum L. Coriandrum) مورد بررسی قرار گرفت، این آزمایش بصورت طرح آماری کاملاً تصادفی در سه تکرار در گلخانه‌ای واقع در شهرستان گرمسار اجرا شد. تیمارهای محلول‌پاشی آلانین، پرولین و گلوتامین با غلظت‌های صفر، 50 و 100 میلی‌گرم در لیتر در دو مرحله 2 تا 4 برگی و 4 تا 6 برگی اعمال شدند. نتایج نشان داد تیمارها به طور معنی‌داری صفات مورد ارزیابی را نسبت به شاهد افزایش دادند، به طوریکه بیشترین درصد شاخص ثبات غشاء سلول و میزان پروتئین، فعالیت آنزیم‌های سوپراکسید دیسموتاز، کاتالاز و پراکسیداز در تیمار پرولین 100 میلی‌گرم در لیتر، بیشترین میزان وزن تر و خشک اندام هوایی و ریشه و میزان ازت در تیمار گلوتامین 100 میلی‌گرم در لیتر و بیشترین محتوای کلروفیل کل برگ در تیمار آلانین 100 میلی‌گرم در لیتر بود. بنابراین با توجه به نتایج این پژوهش می‌توان محلول‌پاشی آلانین، پرولین و گلوتامین خصوصاً با غلظت 100 میلی‌گرم در لیتر را جهت بهبود خصوصیات مورفوفیزیولوژیک و آنزیمی گشنیز توصیه نمود.

چکیده انگلیسی:

Amino acids are biological stimulants that has an effective role in improving the metabolism, growth and performance of plants. In this research, the effect of amino acids alanine, proline and glutamine on some growth, physiological and enzymatic characteristics of coriander (Coriandrum sativum L.) was investigated. this experiment was carried out as a completely random statistical design in three replications in a greenhouse located in Garmsar city. The foliar application of alanine, proline and glutamine with concentrations of zero, 50 and 100 mg l-1 were applied in two stages of 2 to 4 leaves and 4 to 6 leaves. The results showed that the treatments significantly increased the evaluated traits compared to the control, so that the highest percentage of cell membrane stability index and protein amount, the activity of superoxide dismutase, catalase and peroxidase enzymes were in 100 mg l-1 proline treatment, the highest amount of fresh and dry weight shoots and roots and the amount of nitrogen were in 100 mg l-1 glutamine treatment, and the highest total chlorophyll content of leaf were in 100 mg l-1 alanine treatment. Therefore, according to the results of this research, foliar application of alanine, proline and glutamine, especially with a concentration of 100 mg l-1, can be recommended to improve the morphophysiological and enzymatic properties of coriander.

منابع و مأخذ:

1. Amani-Geshnigani S, Shabanian G R. Effect of Coriandrum sativum seed extract on the signs of allergic rhinitis. Feyz. 2013; 17(4): 352-358. [In Persian]


2. Gholizadeh A, Dehghani H, Khodadadi, M. Study on relationships between yield and its components in Iranian coriander genotypes. Journal of Plant Production Re-search. 2018; 25(2): 67-82. [In Persian]


3. Nardi S, Pizzeghello D, Schiavon M, Ertani A. Plant biostimulants: physiolo-gical responses induced by protein hydro-lyzed-based products and humic substan-ces in plant metabolism. Scientia Agricola. 2015; 73(1): 18-23.

doi:10.1590/0103-9016-2015-0006.

4. Rafie M. R, Sohi M, Javadzadeh M. Study the effect of plant growth bios-timulants application on quantitative and qualitative characteristics of wheat in a calcareous soil (Case study in Khuzestan). Journal of Soil Management and Sustain-able Production. 2020; 10(2): 69-88 [In Persian]


5. Faten S.A, Shaheen A.M, Ahmed A.A, Mahmoud A.R. Effect of foliar application of amino acids as antioxidants of growth, yield and characteristics of squash. Res-earch Journal of Agriculture and Biolo-gical Science. 2010; 6(5): 583-588. doi: 10.1093/jxb/32.1.93.


6. Alfosea-Simón M, Simón-Grao S, Zav-ala-Gonzalez EA, Cámara-Zapata JM, Simón I, Martínez-Nicolás JJ, Lidón V, García-Sánchez F. Physiological, nutriti-onnal and metabolomic responses of tom-ato plants after the foliar application of amino acids aspartic acid, glutamic acid and alanine. Frontiers Plant Science. 2021; 11:581234.

doi: 10.3389/fpls.2020.581234.

7. Szabados L, Savoure A. Proline: a mul-tifunctional amino acid. Trends in Plant Sciences. 2009; 15(2): 89-97.


8. Abaspour Esfaden M, Kallaterjari S, Fatehi F. The effect ofsalicylic acid and L-arginine on morpho-physiological proper-ties and leafnutrients of Catharanthus roseus under Drought Stress. Journal of Horticultural Science. 2019; 33(3): 417-432. [In Persian]


9. Kendziorek M, Paszkowski A, Zagdan-ska B. Differential regulation of alanine aminotransferase homologues by abiotic stresses in wheat (Triticum aestivum L.) seedlings. Plant Cell Reports. 2012; 31: 1105–1117.


10. Tajik N, Danaee, E.Spraying pre-har-vest some of amino acids on physicoche-mical, enzymatic traits andlongevity gerb-era flower cv. Sorbet. Iran Plant and Bio-technology journal. 2014; 9(3 and 4): 27-33. [In Persian]


11. Abd-Elkader H. H; Hekmat Y, Masso-ud T.T, El-Erian M.A. Effect of amino acids spray on growth, flowering and keeping quality of Gerbera jamesonii L. as a pot plant. Journal of Plant Production. 2020; 11(2): 201-206.

doi: 10.21608/jpp.2020.79110.

12. Mohammadi Khalifelouiy Z, Abbasi-far A. R, Khadivi A, Akramian M. E. The effect of proline and 24-epibrassinolide on growth indices and biochemical chara-cteristics of the summer savory (Satureja hortensis L.). Journal of Plant Research (Iranian Journal of Biology). 2020; 32(4):-925-940. [In Persian]


13. Noroozlo Y, Souri M, Delshad M. Stimulation Effects of Foliar Applied Gly-cine and Glutamine Amino Acids on Let-tuce Growth. Open Agriculture. 2019; 4(1): 164-172. doi: 10.1515/opag-2019-0016.


14. Danaee E, Abdossi V. Effect of foliar application of iron, potassium, and zinc nano-chelates on nutritional value and essential oil of Basil (Ocimum basilicum L.). Food & Health. 2021; 4(4): 13-20.


15. Jamali Moghadam H, Hassanpour Asil M. Improving morpho-physiological char-acteristics and extending vase life of Lily (Lilium LA Hybrid) cv. Original Love using gibberellic acid and humic acid. Flower and Ornamental Plants. 2021; 6(1): 49-70.


16. Hosseinzadeh Rostam Kalaei M, Abdossi V, Danaee E. Investigation of different selenium sources on phytoche-mical characteristics of Echium amoenum fisch. & C.A. Mey. as a medicinal herb of Iran. Food & Health. 2021; 4(2): 1-7.


17. Tabatabaei S.J. Principles of Plant Mineral Nutrition. Kharazmi, Tabriz, Iran. 2009 [In Persian]


18. Sarcheshmeh poor M, Taheri A, Nasibi F, Bahrami Nejad F. The effect of foliar application of algae extract, beta-carotene and vitamin E on some biochemical parameters of pistachio seedlings under salinity stress. Journal of Quality and Durability of Agricultural Products and Food Stuffs. 2022; 1(4): 1-11. [In Persian]


19. Dhindsa R.S, Plumb-Dhindsa P, Tho-rpe T.A. Leaf senescence: correlated with increased levels of membrane perme-ability and lipid peroxidation, and decr-eased levels of superoxide dismutase and catalase. Journal of Experimental Botany. 1981; 1(32): 93-101.


20. Bayer W. F, Fridovich I. 1987. Ass-aying for superoxide dismutase activity: some large consequences of minor chan-ges in condition. Analytical Biochemistry. 1987; 161: 559-566. doi: 10.1016/0003-2697(87)90489-1.


21. Holy MC. Indole acetic acid oxidase: a dual catalytic enzyme. Plant Physiology. 1972; 50: 15-18. doi: 10.1104/pp.50.1.15.


22. Radkowski A, Radkowska I. Influence of foliar fertilization with amino acid preparations on morphological traits and seed yield of timothy. Plant, Soil and Env-ironment. 2018; 64: 209-213.

doi: 10.17221/112/2018-PSE.

23. Soltani F, Hadavi E, Ghazi Jahani N. The effect of foliar application of glut-amine, citric acid and malic acid on the growth, quality and morphological traits of licorice (Glycyrrhiza glabra(. Iran Plant and Biotechnology journal. 2017; 12(3):5-14. [In Persian]


24. Wael M. S, Abdelsattar A, Mohamed O. A. R,Refat A, Marey T. A, El-Mageed, A. Exogenously applied proline enhances growth andproductivity of drought stre-ssed onion by improvingphotosynthetic efficiency, water use efficiency andup-regulating osmoprotectants. ScientiaHor-ticulturae. 2020: 272 (1): 109580.

doi:10.1016/j.scienta.2020.109580

25. Ghafari H, Tadayon M R, Razmjoo J. Effect foliar of proline on some physio-logical indices of sugar beet (Beta vulgari L.) to water deficit condition. Plant Pro-cess and Function. 2018; 7 (26):13-26. [In Persian]


26. Saremi S, Gholipoor M, Abbasdokht H, Naghdi Badi H, Mehrafarin A, Asghari H. R. The morphophysiological responses of Physalis alkekengi to foliar applications of amino acids under drought stress con-ditions. Horticultural Plants Nutrition. 2020; 3(2): 71-86.

doi: 10.22070/hpn.2020.5377.1091

27. Gendy A.S, Nosir W.S. Improving productivity and chemical constituents of Roselle plant (Hibiscus sabdariffa L.) as affected by phenylalanine, L-tryptophan and peptone acids foliar application. Mid-dle East Journal of Agriculture. 2016; 5(4): 701- 708


28. Vendruscolo E.C, Schuster I, Pileggi M, Scapim C.A, Molinari H.B, Marur C.J, Vieira L.G. Stress-induced synthesis of proline confers tolerance to water deficit in transgenic wheat. Journal of Plant Phy-siology. 2007; 164(10): 1367-1376.


29. Soroori S, Danaee E, Hemmati Kh, Ladan Moghadam, A. The metabolic res-ponse and enzymatic activity of Calendula officinalis L. to foliar application of Spermidine, Citric Acid and Proline under drought stress and in a Post-Harvest. Jour-nal of Agricultural Science and Technol-ogy. 2021; 23(06): 1339-1353.

doi: 20.1001.1.16807073.2021.23.6.6.9.

30. Ghafari H, Tadayon M. R, Razmjoo J. The role of salicylic acid and proline treatment on induction of antioxidant en-zyme activities and salt tolerance respon-ses of soybean (Glycine max L.). Envir-onmental Stresses in Crop Sciences. 2018; 11(3): 691-705 [In Persian]


31. Darvizheh H, Zavareh M, Ghasmanjad M. Effect of proline spraying on biochem-ical properties of German chamomile in water stress conditions (Matricaria cham-omilla L). Journal of Applied Research in Plant Ecophysiology. 2017; 4 (1): 35-60. [In Persian]