Phytochemical study and anti-inflammatory activity of the roots of Mangifera indica L. in lipopolysaccharide (LPS)-stimulated peritoneal macrophages
محورهای موضوعی : Phytochemistry: Isolation, Purification, CharacterizationYolande Nangue 1 , Eulogio José Llorent-Martínez 2 , Maria Luisa Fernández-de Córdova 3 , Douglas Alphonse M. Ngangoum 4 , Télesphore Benoit Nguelefack 5 , Anatole Guy Blaise Azebaze 6 , Alain Bertrand Dongmo 7
1 - Department of Chemistry, Faculty of Sciences, P.O. Box 24157, University of Douala, Cameroon
2 - Department of Physical and Analytical Chemistry, University of Jaén, Campus Las Lagunillas S/N, E-23071 Jaén, Spain
3 - Department of Physical and Analytical Chemistry, University of Jaén, Campus Las Lagunillas S/N, E-23071 Jaén, Spain
4 - Department of Animal Biology, Faculty of Sciences, P.O. Box 67, University of Dschang, Cameroon
5 - Department of Animal Biology, Faculty of Sciences, P.O. Box 67, University of Dschang, Cameroon
6 - Department of Chemistry, Faculty of Sciences, P.O. Box 24157, University of Douala, Cameroon
7 - Department of Animal Biology and Physiology, Faculty of Sciences, P.O. Box 24157, University of Douala, Cameroon
کلید واژه: Cytotoxicity, nitric oxide, Anti-inflammatory, phenolic compounds, <i>Mangifera indica</i>,
چکیده مقاله :
This study deals with the identification of secondary metabolites of methanol extract of the roots of Mangifera indica L and the assessment of plant in vitro anti-inflammatory activity. High-performance liquid chromatography with electrospray ionization mass spectrometric detection (HPLC-ESI-MSn) using the negative ion mode was performed to establish the chromatographic fingerprint and identify various chemical components of the plant extract. The anti-inflammatory effect of the MeOH extract (3, 30 and 300 μg/mL) was assessed through cell viability and nitric oxide (NO) production on non-stimulated and LPS-stimulated peritoneal macrophages. Phytochemical analysis indicated the presence of a number of phenolic compounds where galloyl derivatives, mangiferin and its derivatives were the major constituents. The methanol extract exhibited significant concentration-dependent inhibitory effect on NO production, both on stimulated and non-stimulated macrophages. The concentration 300 μg/mL showed significant cell toxicity. The methanol extract of Mangifera indica is rich in phenolic compounds and possesses potent in vitro anti-inflammatory activity, but its higher concentrations are cytotoxic.
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