The Vaibility of Probiotic Bacteria and Characteristics of Un-cultured Cream Containing Inulin
محورهای موضوعی : food microbiologyA. Farrokh 1 , M. R. Ehsani 2 , N. Moayednia 3 , R. Azizi Nezhad 4
1 - Ph. D. Student of the Department of Food Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
2 - Professor of the Department of Food Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
3 - Assistant Professor of Food Science and Technology, Qazvin Branch, Islamic Azad University, Qazvin, Iran.
4 - Assistant Professor of the Plant Breeding Department, Science and Research Branch, Islamic Azad University, Tehran, Iran.
کلید واژه: Inulin, Lactobacillus acidophilus, Lactobacillus casei, Synbiotic, Sweet Cream,
چکیده مقاله :
The effect of different levels of inulin (0, 1.5 and 3 %) and probiotic bacteria including Lactobacillus acidophilus and Lactobacillus casei on chemical properties (pH and titratable acidity) and viable count of probiotic strain of sweet cream during 30 days for 15-day interval at 4°C were studied. All experiments were carried out in three replications. The results showed that during the refrigeration storage of samples, pH decreased and acidity increased significantly. The addition of inulin caused significant changes in pH, acidity and the viability of probiotic strains. By increasing the level of inulin to 3%, the pH of the cream samples decreased, and the acidity and counts of the probiotics increased significantly and this was more significant in Lactobacillus acidophilus, although the treatment containing Lactobacillus acidophilus had the highest acidity. The probiotic survival evaluation also indicated that inulin treatment at 3% level was the best treatment for increasing the viability of the probiotics in cream. The results showed that the addition of prebiotics, such as inulin, could play an important role in increasing the viability of probiotics. In general, changes in the chemical properties of sweet cream during the refrigeration were standard, which could be considered as a synbiotic product and sweet cream as a dairy product can also be considered as a good carrier for probiotic bacteria.
kin, M. B., Akin, M. S. & Kirmaci, Z. (2007), Effects of inulin and sugar levels on the viability of yogurt and probiotic bacteria and the physical and sensory characteristics in probiotic ice-cream. Food Chemistry, 104, 93- 99.
Alhart, A. J. & Stage, A. (2003). Use of probiotic in tratment of inflamatory bowel
disease. Journal Clinic Gasteroenterol, 36, 111-119.
Blandino, A., Al-Aseeri, M. E., Pandiella, S. S., Cantero, D. & Webb, C. (2003). Review.Cereal-based fermented food and beverages. Food Research International, 36, 527-543.
Bosscher, D., Van Loo, J. & Franck, A. (2006). Inulin and oligofructose as functional ingredients to improve bone mineralization. International Dairy Journal, 16, 1092-1097.
Cross, M. L .(2002). Microbes versus microbes, immune signals generated by probiotic lactobacilli and their role in protection against microbial pathogens. FEMS Immunology and Medical Microbiology, 34, 245-253.
Donkor, O. N., Nilmini, S. L. I., Stolic, P., Vasilgevic, T. & Shah, N. P. (2007). Survival and activity of selected probiotic organism in set – type yoghurt during cold storage. International Dairy Journal, 17, 92-151.
Founden, R., Mogensen, G.,Tanaka, R. & Salminen, R. (2000). Culture-containing dairy product-effect on intestinal microfelora, human nutrition and health-current knowledge and future perspectines, Bull. International Dairy Federation, 35, 21-37.
Gibson, G. R. & Roberfroid, M. B. (2004). Dietary modulation of the human colonic microbiota; introducing the concept of prebiotics. Journal of Nutrition, 125, 1401–1412.
Heenan, C. M., Adams, M. C., Hosken, R. W. & Fleet, G. H. (2004). Survival and sensory acceptability of probiotic microorganisms in a nonfermented frozen vegetarian dessert. Lebensmittel Wissenschaft and Technology, 37, 461-466.
Holzapfel, W. H., Haberer, P., Snel, J., Schillinger, V. & Huis, J. H. J. )1998). Overview of gut flora and probiotics. International Journal of Food Microbiology, 41, 85-101.
Holzapfel, W. H. & Zchillinger, U. (2002). Introduction to pre- and probiotics. Food Research International, 35, 109-116.
Lopez-Molina, D., Navarro-martinez, M. D., melgarejo, F. R., Hiner, A. N. P., Chzarra, S. & Rodriguez-Lopez, J. N. (2005). Molecular properties and Prebiotic effect of inulin obtained from artichoke (Cynara scolymus. L). Phytochemistry, 66, 1476-1484.
Maiocchi, G. (2001). YOMO ABC: Functional
food for consumers well-being and satisfaction. Industrial del Latte, 1-2, 94-98.
Majeed, M. & Prakash, L. )2007(. Probiotics for health and wellbeing. Sabinsa Corporation, 1,12.
Oliveira, R. P. S., Perego, P., Converti, A. & Oliveira, M. N. (2009). Effect of inulin supplementation of milk to prepare fermented biomilks. Journal of Food Science 14, 1-7.
Ostile, H. M., Treimo, J. & Narvhus, J. A. (2005). Effect of temperature on growth and metabolism of probiotic bacteria in milk. International Dairy Journal, 15, 989-997.
Paseephol, T. (2008). Characterisation of prebiotic compounds from plant sources and food industry wastes. Inulin from Jerusalem artichoke and Lactulose from milk concentration permeate, 1, 21.
Pochapin, M. (2000). The effect of probiotics on Clostridium difficile diarrhea. American Journal of Gastroenterology, 95, 11-13.
Rodrigueze, E., Gonzalez, B., Gaya, P., Nunez, M. & Medina, M. (2000). Diversity of bacteriocins produced by lactic acid bacteria isolated from raw milk. International Dairy Journal, 10, 7-15.
Said, J. A. O. & Gilliland, S. E. (2005). Antioxidative activity of Lactobacilli measured by oxygen radical absorbance capacity. Journal of Dairy Science, 88(4), 1352-1357.
Siro, I., Kapolna, E., Kapolna, B. & Lugasia, A. (2008). Functional food. Product development, marketing and consumer acceptance-A review.Appetite, 51, 456-467.
Tamime, A. Y. (2005). Probiotic dairy products. Blackwell Publishing,Oxford, 1, 216.
Vahcic, N. & Hruskar, M. (2000). Slovenian fermented milk with probiotics, Zootehnika, 76, 41-46.
Vijayakumar, J., Aravindan, R. & Viruthagiri, T. (2008). Recent trends in the production, purification and application of lactic acid. Chemical and Biochemical Engineering, 22 (2), 245.
Vincenzo Tufarelli, V. & Vito Laudadio, V. (2016(. An overview on the functional food concept: prospectives and applied researches in probiotics, prebiotics and synbiotics. Journal of Experimental Biology and Agricultural Sciences, 3 (3), 273-278.
Yeganehzad, S., Mazaheri-Tehrani, M. & Shahidi, F. (2007). Studying microbial, physicochemical and sensory properties of directly concentration probiotic yogurt. African Journal of Agricultural Research, 2 (8),366-369.
