Antioxidative Activity and Functional Properties of Enzymatic Protein Hydrolysate of Spirulina platensis
محورهای موضوعی : food biotechnology
M. Forutan
1
,
M. Hasani
2
,
Sh. Hasani
3
,
N. Salehi
4
1 - PhD Student of the Department of Food Science and Technology, Shahrood Branch, Islamic Azad University, Shahrood, Iran.
2 - Assistant Professor of the Department of Food Science and Technology, Shahrood Branch, Islamic Azad University, Shahrood, Iran.
3 - PhD Graduated of the Department of Fisheries, Faculty of Fisheries and the Environment, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
4 - Associate Professor of the Department of Basic Sciences, Shahrood Branch, Islamic Azad University, Shahrood, Iran.
کلید واژه: Spirulina, Solubility, Protein Hydrolysate, Antioxidant Activities,
چکیده مقاله :
This study aimed to evaluate the effect of enzymatic hydrolysis of Spirulina protein on the solubility, emulsifying activity index (EAI), emulsion stability index (ESI), and DPPH and ABTS radical scavenging activity of extracted peptides. The molecular weight of spirulina protein was determined using the SDS-Page technique. The extracted protein was hydrolyzed by alcalase enzyme and the degree of hydrolysis (DH%) was determined after 2-12 h. Functional properties and antioxidant activities of extracted protein and hydrolyzed proteins were evaluated. The concentration of extracted protein was 108 mg/L and the protein content of Spirulina was 54% (w/w). Most of the protein bands were in the molecular weight range of 15 to 20 kDa, and the DH reached from 4.2 ± 2.1% to 14 ± 1% after 10 h of enzymatic hydrolysis. The lowest solubility was recorded for the extracted protein at pH of 4, and the highest was related to the 14% hydrolyzed protein at pH of 8. Moreover, the extracted protein had higher EAI and ESI than hydrolyzed proteins, and the effect of pH was more evident on the EAI of hydrolyzed proteins compared to ESI. The present study showed that the antioxidant activity of the protein increased with increasing degree of hydrolysis and its concentration. Moreover, the protein with a DH of 14% in all concentrations had the highest inhibition. This work presented that the current method used for extraction and enzymatic hydrolysis of spirulina protein led to the production of peptides that have desirable properties for use in the food industry.
Abreu, M. H., Pereira, R. & Sassi, J. F.( 2014). Marine algae and the global food industry. Marine algae, biodiversity, taxonomy, environmental assessment, and biotechnology. CRC Press, Bocca Raton, FL, pp.300-319.
Afify, A. E-M. M. R., Baroty, G. S. E., Baz, F. K. E., Baky, H. H. A. E. & Murad, S. A. (2018). Scenedesmus obliquus: Antioxidant and antiviral activity of proteins hydrolyzed by three enzymes. Journal of Genetic Engineering and Biotechnology,16(2), 399-408.
Agarwal, S., Beausire, R. L., Patel, S. & Patel, H. (2015). Innovative uses of milk protein concentrates in product development. Journal of food science, 80(51), A23-A29.
Alzahrani, M. A. J., Peausire, C. O. & Hemar, Y. (2018). Production of bioactive proteins and peptides from the diatom Nitzschia laevis and comparison of their in vitro antioxidant activities with those from Spirulina platensis and Chlorella vulgaris. International Journal of Food Science & Technology, 53(3), 676-682.
Anjum, K., Abbas, S. Q., Akhter, N., Shagufta, B. I., Shah, S. A. A. & Hassan, S. S. U. (2017). Emerging biopharmaceuticals from bioactive peptides derived from marine organisms. Chemical biology & drug design, 90(1), 12-30.
Bashir, S., Sharif, M. K., Butt, M. S. & Shahid, M. (2016). Functional properties and amino acid profile of Spirulina platensis protein isolates. Biological Sciences-PJSIR, 59(1), 12-19.
Becker, E. W. (2007). Micro-algae as a source of protein. Biotechnology advances, 25(2), 207-210.
Benelhadj, S., Gharsallaoui, A., Degraeve, P., Attia, H. & Ghorbel, D. (2016). Effect of pH on the functional properties of Arthrospira (Spirulina) platensis protein isolate. Food Chemistry, 194, 1056-1063.
Cavonius, L. R., Albers, E. & Undeland, I. (2015). pH-shift processing of Nannochloropsis oculata microalgal biomass to obtain a protein-enriched food or feed ingredient. Algal research, 11, 95-102.
Cermeno, M., Kleekayai, T., Amigo‐Benavent, M., Harnedy‐Rothwell, P. & Fitzgerald, R. J. (2020). Current knowledge on the extraction, purification, identification, and validation of bioactive peptides from seaweed. Electrophoresis, 41(20), 1694-1717.
Chang, C., Li, X., Li, J., Niu, F., Zhang, M., Zhou, B., Su, Y. & Yang, Y. (2017). Effect of enzymatic hydrolysis on characteristics and synergistic efficiency of pectin on emulsifying properties of egg white protein. Food Hydrocolloids, 65, 87-95.
Chatterjee, R., Dey, T. K., Ghosh, M. & Dhar, P. (2015). Enzymatic modification of sesame seed protein, sourced from waste resource for nutraceutical application. Food and Bioproducts Processing, 94, 70-81.
Chen, Y., Chen, J., Chanj, C., Chen, J., Cao, F., Zhao, J., Zheng, Y. & Zhu, J. (2019). Physicochemical and functional properties of proteins extracted from three microalgal species. Food Hydrocolloids, 96, 510-517.
Church, F. C., Swaisgood, H. E., Porter, D. H. & Catignani, G. L. (1983). Spectrophotometric assay using o-phthaldialdehyde for determination of proteolysis in milk and isolated milk proteins. Journal of dairy science, 66(6), 1219-1227.
Cian, R. E., Martinez-Augustin, O. & Drago, S. R. (2012). Bioactive properties of peptides obtained by enzymatic hydrolysis from protein byproducts of Porphyra columbina. Food Research International, 49(1), 364-372.
Ebert, S., Grossmann, L., Hinrichs, J. & Weiss, J. (2019). Emulsifying properties of water-soluble proteins extracted from the microalgae Chlorella sorokiniana and Phaeodactylum tricornutum. Food & function, 10(2), 754-764.
Eckert, E., Han, J., Swallow, K., Tian, Z., Jarpa‐Parra, M. & Chen, L. (2019). Effects of enzymatic hydrolysis and ultrafiltration on physicochemical and functional properties of faba bean protein. Cereal Chemistry, 96(4), 725-741.
Fan, X., Cui, Y., Zhang, R. & Zhang, X. (2018). Purification and identification of anti-obesity peptides derived from Spirulina platensis. Journal of Functional Foods, 47, 350-360.
Fouda, W. A., Ibrahim, W. M., Ellamie, A. M. & Ramadan, G. (2019). Biochemical and mineral compositions of six brown seaweeds collected from Red Sea at Hurghada Coast. Indian Journal of Geo Marine Sciences, 48(4), 484-491.
Gomes, M. H. G. & Kurozawa, L. E. (2020). Improvement of the functional and antioxidant properties of rice protein by enzymatic hydrolysis for the microencapsulation of linseed oil. Journal of Food Engineering, 267, 109761.
Hayes, M. (2018). Industrial Processing of Proteins. Novel Proteins for Food, Pharmaceuticals, and Agriculture, 281-290.
Jamdar, S., Rajalakshmi, V., Pednekar, M., Juan, F., Yardi, V. & Sharma, A. (2010). Influence of degree of hydrolysis on functional properties, antioxidant activity and ACE inhibitory activity of peanut protein hydrolysate. Food chemistry, 121(1), 178-184.
Klompong, V., Benjakul, S., Kantachote, D. & Shahidi, F. (2007). Antioxidative activity and functional properties of protein hydrolysate of yellow stripe trevally (Selaroides leptolepis) as influenced by the degree of hydrolysis and enzyme type. Food chemistry, 102(4), 1317-1327.
Laemmli, U. K.( 1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. nature, 227(5259), 680-685.
LIU, X. & Kokare, C. (2017). Microbial enzymes of use in industry. Biotechnology of microbial enzymes,267-298.
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. Journal of biological chemistry, 193(1), 265-275.
Maehre, H. K., Malde, M. K., Eilertsen, K. E. & Elvevoll, E. O. (2014). Characterization of protein, lipid and mineral contents in common Norwegian seaweeds and evaluation of their potential as food and feed. Journal of the Science of Food and Agriculture, 94(15), 3281-3290.
Martinez- Andrade, K. A., Lauritano, C., Romano, G. & Ianora, A. (2018). Marine microalgae with anti-cancer properties. Marine Drugs, 16(5), 165.
Moraes, C. C., Sala, L., Cerveira, G. P. & Kalil, S. J. (2011). C-phycocyanin extraction from Spirulina platensis wet biomass. Brazilian Journal of Chemical Engineering, 28(1), 45-49.
Mostolizadeh, S. S., Moradi, Y., Mortazavi, M. S., Motallebi, A. A. & Ghaeni, M. (2020). Effects of incorporation Spirulina platensis (Gomont, 1892) powder in wheat flour on chemical, microbial and sensory properties of pasta. Iranian Journal of Fisheries Sciences, 19(1), 410-420.
Nasri, R., Younes, I., Jridi, M., Trigui, M., Bougatef, A., Nedjar-Arroume, N., Dhulster, P., Nasri, M. & Karra-Chaabouni, M.(2013). ACE inhibitory and antioxidative activities of Goby (Zosterissessor ophiocephalus) fish protein hydrolysates: effect on meat lipid oxidation. Food Research International, 54(1), 552-561.
Ngo, D. H., Vo, T. S., Ngo, D. N., Wijesekara, I. & Kim, S. K. (2012). Biological activities and potential health benefits of bioactive peptides derived from marine organisms. International journal of biological macromolecules, 51(4), 378-383.
Nwachukwu, I. D. & Aluko, R. E. (2019). Structural and functional properties of food protein‐derived antioxidant peptides. Journal of Food Biochemistry, 43(1), e12761.
Parimi, N. S., Singh, M., Kastner, J. R., Das, K. C., Forsberg, L. S. & Azadi, P. (2015). Optimization of protein extraction from Spirulina platensis to generate a potential co-product and a biofuel feedstock with reduced nitrogen content. Frontiers in Energy Research, 3, 30.
Pearce, K. N. & Kinsella, J. E. (1978). Emulsifying properties of proteins: evaluation of a turbidimetric technique. Journal of Agricultural and Food Chemistry, 26(3), 716-723.
Pereira, A. M., Lisboa, C. R. & Costa, J. A. V. (2018). High protein ingredients of microalgal origin: Obtainment and functional properties. Innovative Food Science & Emerging Technologies, 47, 187-194.
Pereira, A. M., Lisboa, C. R., Santos, T. D. & Costa, J. A. V. (2019). Bioactive stability of microalgal protein hydrolysates under food processing and storage conditions. Journal of food science and technology, 56(10), 4543-4551.
Rajakumar, M. S. & Muthukumar, K. (2018). Influence of pre-soaking conditions on ultrasonic extraction of Spirulina platensis proteins and its recovery using aqueous biphasic system. Separation Science and Technology, 53(13), 2034-2043.
Salinas-Salazar, C., Garcia-Perez, J. S., Chandra, R., Castillo-Zacarisa, C., Iqbal, H. M. & Parra-Saldivar, R. (2019). Methods for Extraction of Valuable Products from Microalgae Biomass. Microalgae biotechnology for development of biofuel and wastewater treatment. Springer. pp 245-263.
Samarakoon, K. & Jeon, Y. J. (2012). Bio-functionalities of proteins derived from marine algae-A review. Food Research International, 48(2), 948-960.
Sanmartin, B., Diaz, O., Rodriguez-Turienzo, L. & Cobos, A. (2013). Functional properties of caprine whey protein concentrates obtained from clarified cheese whey. Small Ruminant Research, 110(1), 52-56.
Schröder, A., Berton-Carabin, C., Venema, P. & Cornacchia, L. (2017). Interfacial properties of whey protein and whey protein hydrolysates and their influence on O/W emulsion stability. Food Hydrocolloids, 73, 129-140.
Schwenzfeier, A., Wierenga, P. A. & Gruppen, H. (2011). Isolation and characterization of soluble protein from the green microalgae Tetraselmis sp. Bioresource technology, 102(19), 9121-9127.
Sedighi, M., Jalili, H., Darvish, M., Sadeghi, S. & Ranaei-Siadat, S. O. (2019). Enzymatic hydrolysis of microalgae proteins using serine proteases: A study to characterize kinetic parameters. Food chemistry, 284, 334-339.
Sharma, G., Kumar, M., Ali, M. I., Saran, S. & Jasuja, N. D. (2015). Impact of natural light on growth and biopigment profile of cyanobacteria Spirulina platensis. Journal of environmental biology, 36(6), 1389-1392.
Tabarsa, M., Rezaei, M., Ramezanpour, Z., Robert- Waaland, J. & Rabiei, R. (2012). Fatty acids, amino acids, mineral contents, and proximate composition of some brown seaweeds . Journal of phycology, 48(2), 285-292.
Teshnizi, Z. M., Robatjazi, S. M. & Mosaabadi, J. M. (2020). Optimization of the Enzymatic Hydrolysis of Poultry Slaughterhouse Wastes using Alcalase Enzyme for the Preparation of Protein Hydrolysates. Applied Food Biotechnology, 7(3), 153-160.
Toopcham, T., Mes, J. J., Wichers, H. J., Roytrakul, S. & Yongsawatdigul, J. (2017). Bioavailability of angiotensin I-converting enzyme (ACE) inhibitory peptides derived from Virgibacillus halodenitrificans SK1-3-7 proteinases hydrolyzed tilapia muscle proteins. Food chemistry, 220, 190-197.
Wang, X. & Zhang, X. (2012). Optimal extraction and hydrolysis of Chlorella pyrenoidosa proteins. Bioresource technology, 126, 307-313.
Williams, P. J. L. B. & Laurens, L. M. (2010). Microalgae as biodiesel & biomass feedstocks: Review & analysis of the biochemistry, energetics & economics. Energy & Environmental Science, 3(5), 554-590.
Zhu, Y., Zhao, X., Zhang, X., Liu, H. & Ao, Q. (2021). Amino acid, structure and antioxidant properties of Haematococcus pluvialis protein hydrolysates produced by different proteases. International Journal of Food Science & Technology, 56, 185-195.
