تعیین الگوی مقاومت آنتی بیوتیکی و بررسی فراوانی ژن‌های اوپرون ica در سویه های استافیلوکوکوس ساپروفیتیکوس جدا شده از موارد کلینیکی شهرستان شهرکرد
محورهای موضوعی : میکروبیولوژیفروغ صدیقی 1 , حسین آقاجانی 2 , حسین خدابنده شهرکی 3 , فاطمه نعمت الهی 4 , الهه تاج بخش 5
1 - 1.گروه میکروبیولوژی، دانشکده علوم پایه، دانشگاه آزاد اسلامی، واحد شهرکرد، شهرکرد، ایران
2 - گروه میکروبیولوژی، دانشکده علوم پایه، دانشگاه آزاد اسلامی، واحد شهرکرد، شهرکرد، ایران
3 -
4 - گروه میکروبیولوژی، دانشکده علوم پایه، دانشگاه آزاد اسلامی، واحد شهرکرد، شهرکرد، ایران
5 - گروه زیست شناسی دانشگاه آزاد اسلامی واحد شهرکرد
کلید واژه: استافیلوکوکوس ساپروفیتیکوس, اوپرون ica, عفونت ادراری, ژن¬های مقاومت آنتی¬بیوتیکی,
چکیده مقاله :
استافیلوکوکوس ساپروفیتیکوس یکی از عوامل عفونت مجاری ادراری میباشد. با توجه به گستردگی سویهها و وجود عوامل بیماری‌زا هزینههای بالایی جهت جداسازی، تشخیص و درمان این باکتری صورت میگیرد. این مطالعه بهمنظور تعیین الگوی مقاومت آنتیبیوتیکی و فراوانی ژن‌های اوپرون ica در ایزولههای استافیلوکوکوس ساپروفیتیکوس جدا شده از مواد عفونت ادراری در شهرستان شهرکرد انجام گرفت. این تحقیق بر روی 34 ایزوله استافیلوکوکوس ساپروفیتیکوس جدا شده از موارد عفونت ادراری در شهرستان شهرکرد صورت گرفت. بعد از انجام تست‌های بیوشیمیایی بهمنظور تشخیص باکتری، الگوی مقاومت آنتیبیوتیکی به روش کربی بائر بررسی گردید. جهت بررسی فراوانی ژن‌های tetK ، ant(4)-Ia ،vanA، ermA و icaA/C/D/B/R آزمون واکنش زنجیره‌ای پلی مراز با استفاده از زوج پرایمرهای اختصاصی صورت گرفت. بیشترین مقاومت نسبت به پنی سیلین (2/91 درصد) و کمترین مقاومت نسبت به وانکومایسین (9/5 درصد) گزارش شد. فراوانی ژن tetK 7/64 درصد، ermA 88/55 درصد ، ant(4)-Ia 58/20 درصد و vanA صفر درصد گزارش گردید. در 25 ایزوله ژن icaA ( 52/73 درصد)، در 22 ایزوله icaD (70/64 درصد) در 7 ایزوله icaC ( 58/20 درصد ) در 5 ایزوله icaB ( 7/14 درصد) و در 5 ایزوله icaR (7/14 درصد) گزارش گردید.
نتایج نشان میدهد استافیلوکوکوس ساپروفیتیکوس نسبت به تعدادی از آنتیبیوتیکهای انتخابی درمان عفونتها مقاومت دارد. عدم وجود ارتباط آماری معنی دار بین مقاومت با دیسک و مقاومت به روش مولکولی نشان دهنده انتقال مقاومت آنتیبیوتیکی در بین ایزولهها از طریق عوامل دیگری نظیر اینتگرونها و ترانسپوزونها میباشد
Staphylococcus saprophyticus is one of the causes of urinary tract infections in humans. Due to the wide range of strains and the presence of pathogens, high costs are incurred for the isolation, diagnosis and treatment of this bacterium. The aim of this study was to determine the antibiotic resistance pattern and prevalence of ica operon genes in the Staphylococcus saprophyticus strains isolated from clinical samlea in Shahrekord. This study was conducted on 34 strains of Staphylococcus saprophyticus isolated from urinary tract infection samples in Shahrekord city. After bacterial identification using biochemical tests, antibiotic resistance pattern was determined by Kirby-Bauer method. Then polymerase chain reaction (PCR) test was performed using specific primers to detect tetK, ant(4)-Ia ،vanA، ermA and icaA/C/D/B/R genes. Of 34 strains of Staphylococcus saprophyticus isolated from infectious samples, the most commonly detected antibiotic resistance was against penicillin (91.2%) and the most sensitivity was achieved against vancomycin (5.9%). The distribution of tet K, erm A, ant(4)Ia and van A, ica A, ica D, ica C, ica B and ica R were reported 20.58%, 55.88%, 20.58%, 0%, 73.52%, 64.70%, 20.58%, 14.7% and 14.7% respectively. The results that show Staphylococcus saprophyticus is resistance to selected antibiotics. The lack of significant statistical relationship between disc resistance and molecular resistance is indicated that the transfer of antibiotic resistance among isolates through other factors such as introns and transposons.
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