Determination of antibiotic resistant pattern and detection of ica operon genes in Staphylococcus Saprophyticus isolated from clinical samples in Shahrekord, Iran
Subject Areas : microbiologyforough sedighi 1 , hossein Aghajani 2 , Hossein Khodabandeh shahraki 3 , fatemeh nematollahi 4 , Elahe Tajbakhsh 5
1 - 1. Department of Microbiology, Faculty of Basic Sciences, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran
2 - 1. Department of Microbiology, Faculty of Basic Sciences, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran
3 -
4 - Department of Microbiology, Faculty of Basic Sciences, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran
5 - گروه زیست شناسی دانشگاه آزاد اسلامی واحد شهرکرد
Keywords: Antibiotic resistance genes, Staphylococcus saprophyticcus, ica operon, Urinary Tract Infection ,
Abstract :
Staphylococcus saprophyticus is one of the causes of urinary tract infections in humans. Due to the wide range of strains and the presence of pathogens, high costs are incurred for the isolation, diagnosis and treatment of this bacterium. The aim of this study was to determine the antibiotic resistance pattern and prevalence of ica operon genes in the Staphylococcus saprophyticus strains isolated from clinical samlea in Shahrekord. This study was conducted on 34 strains of Staphylococcus saprophyticus isolated from urinary tract infection samples in Shahrekord city. After bacterial identification using biochemical tests, antibiotic resistance pattern was determined by Kirby-Bauer method. Then polymerase chain reaction (PCR) test was performed using specific primers to detect tetK, ant(4)-Ia ،vanA، ermA and icaA/C/D/B/R genes. Of 34 strains of Staphylococcus saprophyticus isolated from infectious samples, the most commonly detected antibiotic resistance was against penicillin (91.2%) and the most sensitivity was achieved against vancomycin (5.9%). The distribution of tet K, erm A, ant(4)Ia and van A, ica A, ica D, ica C, ica B and ica R were reported 20.58%, 55.88%, 20.58%, 0%, 73.52%, 64.70%, 20.58%, 14.7% and 14.7% respectively. The results that show Staphylococcus saprophyticus is resistance to selected antibiotics. The lack of significant statistical relationship between disc resistance and molecular resistance is indicated that the transfer of antibiotic resistance among isolates through other factors such as introns and transposons.
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